Sheep Polyclonal Choline Acetyltransferase antibody. Suitable for ICC/IF and reacts with Rat, Pig samples. Cited in 39 publications. Immunogen corresponding to Synthetic Peptide within Pig CHAT aa 150-200 conjugated to Keyhole Limpet Haemocyanin.
Images
Publications
Filter by
- Cell death discovery 9:1132023ERK MAPK signaling pathway inhibition as a potential target to prevent autophagy alterations in Spinal Muscular Atrophy motoneurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlba Sansa,Maria P Miralles,Maria Beltran,Ferran Celma-Nos,Jordi Calderó,Ana Garcera,Rosa M SolerPubMed 37019880
- Bioengineering & translational medicine 8:e104482023Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Jin,Yayu Wu,Haipeng Zhang,Bai Xu,Wenbin Liu,Chunnan Ji,Panpan Li,Zhenni Chen,Bing Chen,Jiayin Li,Xianming Wu,Peipei Jiang,Yali Hu,Zhifeng Xiao,Yannan Zhao,Jianwu DaiPubMed 36925694
- Frontiers in cellular neuroscience 16:10542702022Survival motor neuron protein and neurite degeneration are regulated by Gemin3 in spinal muscular atrophy motoneurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria P Miralles,Alba Sansa,Maria Beltran,Rosa M Soler,Ana GarceraPubMed 36619669
- Acta pharmacologica Sinica 44:92-1042022TGFβR-1/ALK5 inhibitor RepSox induces enteric glia-to-neuron transition and influences gastrointestinal mobility in adult mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChang-Jie Shi,Jun-Jiang Lian,Bo-Wen Zhang,Jia-Xue Cha,Qiu-Hong Hua,Xiao-Ping Pi,Yu-Jun Hou,Xin Xie,Ru ZhangPubMed 35794374
- Cell reports 39:1108822022A cholinergic medial septum input to medial habenula mediates generalization formation and extinction of visual aversion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRonghao Mu,Susu Tang,Xiaomeng Han,Hao Wang,Danhua Yuan,Jiajia Zhao,Yan Long,Hao HongPubMed 35649349
- eNeuro 9:2022Kv3 Channels Contribute to the Excitability of Subpopulations of Spinal Cord Neurons in Lamina VII.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPierce N Mullen,Nadia Pilati,Charles H Large,Jim Deuchars,Susan A DeucharsPubMed 35058310
- Proceedings of the National Academy of Sciences of the United States of America 118:2021Astrocyte-derived neurons provide excitatory input to the adult striatal circuitry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthijs C Dorst,María Díaz-Moreno,David O Dias,Eduardo L Guimarães,Daniel Holl,Jannis Kalkitsas,Gilad Silberberg,Christian GöritzPubMed 34389674
- Journal of neuroscience research 99:3084-31002021Laterodorsal tegmentum-ventral tegmental area projections encode positive reinforcement signals.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBárbara Coimbra,Ana Verónica Domingues,Carina Soares-Cunha,Raquel Correia,Luísa Pinto,Nuno Sousa,Ana João RodriguesPubMed 34374447
- Acta neuropathologica communications 9:1222021Spinal Muscular Atrophy autophagy profile is tissue-dependent: differential regulation between muscle and motoneurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlba Sansa,Ivan Hidalgo,Maria P Miralles,Sandra de la Fuente,M Jose Perez-Garcia,Francina Munell,Rosa M Soler,Ana GarceraPubMed 34217376
- Communications biology 4:6032021Top-down acetylcholine signaling via olfactory bulb vasopressin cells contributes to social discrimination in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHajime Suyama,Veronica Egger,Michael LukasPubMed 34021245
Key facts
- Isotype
- IgG
- Host species
- Sheep
- Storage buffer
- Standard buffer
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Pig CHAT aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P13222
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Rat | Tested |
| Chicken | Predicted |
| Pig | Expected |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken | Dilution info - | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
2 products for Alternative Version
Target data
Function
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Alternative names
Choline O-acetyltransferase, CHOACTase, ChAT, Choline acetylase, Chat
Recommended products
Sheep Polyclonal Choline Acetyltransferase antibody. Suitable for ICC/IF and reacts with Rat, Pig samples. Cited in 39 publications. Immunogen corresponding to Synthetic Peptide within Pig CHAT aa 150-200 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Sheep
- Storage buffer
- Standard buffer
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Pig CHAT aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P13222
- Purification technique
- Precipitation Ammonium Sulphate
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Add glycerol to a final volume of 50% for extra stability and aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Choline Acetyltransferase (ChAT) also known as EC 2.3.1.6 acts as the enzyme responsible for synthesizing the neurotransmitter acetylcholine. It catalyzes the transfer of an acetyl group from acetyl-CoA to choline to form acetylcholine. ChAT is a protein with an approximate mass of 70 kDa and is expressed in cholinergic neurons throughout the central and peripheral nervous systems. High expression is observed in regions such as the basal forebrain and neuromuscular junctions which are important for cholinergic signaling.
- Biological function summary
The enzyme plays an important role in cholinergic conduction by producing acetylcholine which functions in synaptic transmission. It does not function as part of a larger complex but its activity directly influences cholinergic synapses. Choline acetyltransferase function ensures the synthesis and availability of acetylcholine for release into the synaptic cleft initiating muscle contraction and modulating activity in the central nervous system.
- Pathways
Choline acetyltransferase is critical in pathways governing neurotransmitter release such as the cholinergic synapse pathway. It interacts functionally with other proteins within this pathway including acetylcholinesterase which breaks down acetylcholine in the synaptic cleft. This interaction ensures proper neurotransmitter regulation and recycling maintaining synapse function and communication within neuronal circuits.
- Associated diseases and disorders
Alterations in choline acetyltransferase activity relate to neurological disorders including Alzheimer's disease and myasthenia gravis. In Alzheimer's disease reduced choline acetyltransferase function leads to decreased acetylcholine synthesis contributing to the cognitive decline observed in patients. In myasthenia gravis acetylcholine receptors are impaired but effective choline acetyltransferase activity is still necessary to manage neurotransmitter levels. A potential therapeutic angle involves targeting related proteins that exacerbate these conditions to support acetylcholine levels.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody (ab18736)
Immunofluorescence of Rat Forebrain tissue labeling ChAT with ab18736 using a 1μg dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com