AB18736

Anti-Choline Acetyltransferase antibody

Name: Anti-Choline Acetyltransferase antibody (ab18736) | Abcam
Brand: Abcam Limited
SKU: AB18736
Price: 500 USD
Availability: InStock
Rating: 4 (6 reviews)

(6 Reviews)

(49 Publications)

Sheep Polyclonal Choline Acetyltransferase antibody. Suitable for ICC/IF and reacts with Rat, Pig samples. Cited in 49 publications. Immunogen corresponding to Synthetic Peptide within Pig CHAT aa 150-200 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Choline O-acetyltransferase, CHOACTase, ChAT, Choline acetylase, Chat

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody (AB18736)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody (AB18736)

Immunofluorescence of Rat Forebrain tissue labeling ChAT with ab18736 using a 1μg dilution.

Key facts

Host species

Sheep

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Pig

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Pig CHAT aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P13222

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Chicken": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Precipitation Ammonium Sulphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Add glycerol to a final volume of 50% for extra stability and aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Choline Acetyltransferase (ChAT) also known as EC 2.3.1.6 acts as the enzyme responsible for synthesizing the neurotransmitter acetylcholine. It catalyzes the transfer of an acetyl group from acetyl-CoA to choline to form acetylcholine. ChAT is a protein with an approximate mass of 70 kDa and is expressed in cholinergic neurons throughout the central and peripheral nervous systems. High expression is observed in regions such as the basal forebrain and neuromuscular junctions which are important for cholinergic signaling.

Biological function summary

The enzyme plays an important role in cholinergic conduction by producing acetylcholine which functions in synaptic transmission. It does not function as part of a larger complex but its activity directly influences cholinergic synapses. Choline acetyltransferase function ensures the synthesis and availability of acetylcholine for release into the synaptic cleft initiating muscle contraction and modulating activity in the central nervous system.

Pathways

Choline acetyltransferase is critical in pathways governing neurotransmitter release such as the cholinergic synapse pathway. It interacts functionally with other proteins within this pathway including acetylcholinesterase which breaks down acetylcholine in the synaptic cleft. This interaction ensures proper neurotransmitter regulation and recycling maintaining synapse function and communication within neuronal circuits.

Alterations in choline acetyltransferase activity relate to neurological disorders including Alzheimer's disease and myasthenia gravis. In Alzheimer's disease reduced choline acetyltransferase function leads to decreased acetylcholine synthesis contributing to the cognitive decline observed in patients. In myasthenia gravis acetylcholine receptors are impaired but effective choline acetyltransferase activity is still necessary to manage neurotransmitter levels. A potential therapeutic angle involves targeting related proteins that exacerbate these conditions to support acetylcholine levels.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

See full target information Chat

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Publications (49)

Recent publications for all applications. Explore the full list and refine your search

ACS omega 10:30327-30334 PubMed40727794

2025

Early Exposure of an Infantile Rat to Sex-Related Content Induces Precocious Puberty by Activation of Cholinergic Neurons in the Amygdala and KNDy Neurons in the Arcuate Nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Yifen Shen,Tao Yang,Le Zhang,Yanping Hu,Hao Shen,Hua Li,Yi Yang,Shasha Zhou,Yihang Shen

Frontiers in physiology 16:1540465 PubMed40177363

2025

Aging-related impairment of neurogenic chloride secretion in human colon mucosa.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiyun Wang,Jing He,Pengcheng Yang,Tao Bai,Jun Song,Xiaohua Hou,Lei Zhang

CNS neuroscience & therapeutics 30:e14808 PubMed38887205

2024

Mapping of afferent and efferent connections of phenylethanolamine N-methyltransferase-expressing neurons in the nucleus tractus solitarii.

Applications

Unspecified application

Species

Unspecified reactive species

Mengchu Zhu,Shirui Jun,Xiaojun Nie,Jinting Chen,Yinchao Hao,Hongxiao Yu,Xiang Zhang,Lu Sun,Yuelin Liu,Xiangshan Yuan,Fang Yuan,Sheng Wang

Skeletal muscle 14:5 PubMed38454511

2024

Motor neurons and endothelial cells additively promote development and fusion of human iPSC-derived skeletal myocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Suradip Das,Melanie C Hilman,Feikun Yang,Foteini Mourkioti,Wenli Yang,D Kacy Cullen

EMBO reports 24:e56525 PubMed37534581

2023

ENGRAILED-1 transcription factor has a paracrine neurotrophic activity on adult spinal α-motoneurons.

Applications

Unspecified application

Species

Unspecified reactive species

Mélanie Lebœuf,Stephanie E Vargas-Abonce,Eugénie Pezé-Hedsieck,Edmond Dupont,Lucia Jimenez-Alonso,Kenneth L Moya,Alain Prochiantz

Zoological research 44:712-724 PubMed37313848

2023

Scopolamine causes delirium-like brain network dysfunction and reversible cognitive impairment without neuronal loss.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Wang,Xiang Zhang,Yu-Jie Guo,Ya-Yan Pang,Jun-Jie Li,Yan-Li Zhao,Jun-Fen Wei,Bai-Ting Zhu,Jing-Xiang Tang,Yang-Yang Jiang,Jie Meng,Ji-Rong Yue,Peng Lei

Cell death discovery 9:113 PubMed37019880

2023

ERK MAPK signaling pathway inhibition as a potential target to prevent autophagy alterations in Spinal Muscular Atrophy motoneurons.

Applications

Unspecified application

Species

Unspecified reactive species

Alba Sansa,Maria P Miralles,Maria Beltran,Ferran Celma-Nos,Jordi Calderó,Ana Garcera,Rosa M Soler

Bioengineering & translational medicine 8:e10448 PubMed36925694

2023

Spinal cord tissue engineering using human primary neural progenitor cells and astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Jin,Yayu Wu,Haipeng Zhang,Bai Xu,Wenbin Liu,Chunnan Ji,Panpan Li,Zhenni Chen,Bing Chen,Jiayin Li,Xianming Wu,Peipei Jiang,Yali Hu,Zhifeng Xiao,Yannan Zhao,Jianwu Dai

Frontiers in cellular neuroscience 16:1054270 PubMed36619669

2022

Survival motor neuron protein and neurite degeneration are regulated by Gemin3 in spinal muscular atrophy motoneurons.

Applications

Unspecified application

Species

Unspecified reactive species

Maria P Miralles,Alba Sansa,Maria Beltran,Rosa M Soler,Ana Garcera

International neurourology journal 26:201-209 PubMed36203252

2022

Functional and Immunofluorescence Evaluations of Vascular and Neural Integrities in Urinary Bladder of Streptozotocin-Induced Diabetic Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mi-Hye Kwon,Min-Ji Choi,Fang-Yuan Liu,Fitri Rahma Fridayana,Lashkari Niloofar,Guo Nan Yin,Ji-Kan Ryu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Choline Acetyltransferase antibody

Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody (AB18736)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (49)

Product promise