AB223346

Anti-Choline Acetyltransferase antibody

Name: Anti-Choline Acetyltransferase antibody (ab223346) | Abcam
Brand: Abcam Limited
SKU: AB223346
Rating: 4 (1 reviews)

(1 Review)

(6 Publications)

Rabbit Polyclonal Choline Acetyltransferase antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human CHAT.

View Alternative Names

Choline O-acetyltransferase, CHOACTase, ChAT, Choline acetylase, CHAT

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody (AB223346)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody (AB223346)

4% formaldehyde-fixed SK-N-BE cells stained for Choline Acetyltransferase (green) using ab223346 at 1/100 dilution in ICC/IF. Secondary : Goat Anti-Rabbit ATTO 488 at 1/100 for 60 minutes at RT. Counterstain : Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.

(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223346 (D) Merge.

AbReview72627****

Immunocytochemistry - Anti-Choline Acetyltransferase antibody (AB223346)

Immunocytochemistry analysis of paraformaldehyde-fixed human cultured Neurons, derived from Neuronal Progenitor Cells permeabilized with 0,3% Triton X100 for 5 min, staining with ab223346. Cells were treated with Quenching with Glycin before the permeabilization. Secondary antibody was Fab2 Fragment Cy3™ at 1/500 dilution. The cells were incubated with the primary antibody 1/200 in DAKO Diluent for 1 hour 15 minutes. Blocking was done using 100% DAKO Diluent for 20 minutes at room temperature. In the picture we stained an additional Map-2 marker in green, in red we see the reviewed ChAT- Antibody.

This image is courtesy of an Abreview submitted by Elisabeth Barski

Supplier Data

Western blot - Anti-Choline Acetyltransferase antibody (AB223346)

Blocking : 5% milk + TBST for 1 hour at RT.

Color Development : TMB solution for 12 minutes at RT.

All lanes:

Western blot - Anti-Choline Acetyltransferase antibody (ab223346) at 1/1000 dilution

All lanes:

Mouse brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit: HRP at 1/2000 dilution

Predicted band size: 82 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human CHAT. The exact immunogen used to generate this antibody is proprietary information.

P28329

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Choline Acetyltransferase (ChAT) also known as EC 2.3.1.6 acts as the enzyme responsible for synthesizing the neurotransmitter acetylcholine. It catalyzes the transfer of an acetyl group from acetyl-CoA to choline to form acetylcholine. ChAT is a protein with an approximate mass of 70 kDa and is expressed in cholinergic neurons throughout the central and peripheral nervous systems. High expression is observed in regions such as the basal forebrain and neuromuscular junctions which are important for cholinergic signaling.

Biological function summary

The enzyme plays an important role in cholinergic conduction by producing acetylcholine which functions in synaptic transmission. It does not function as part of a larger complex but its activity directly influences cholinergic synapses. Choline acetyltransferase function ensures the synthesis and availability of acetylcholine for release into the synaptic cleft initiating muscle contraction and modulating activity in the central nervous system.

Pathways

Choline acetyltransferase is critical in pathways governing neurotransmitter release such as the cholinergic synapse pathway. It interacts functionally with other proteins within this pathway including acetylcholinesterase which breaks down acetylcholine in the synaptic cleft. This interaction ensures proper neurotransmitter regulation and recycling maintaining synapse function and communication within neuronal circuits.

Alterations in choline acetyltransferase activity relate to neurological disorders including Alzheimer's disease and myasthenia gravis. In Alzheimer's disease reduced choline acetyltransferase function leads to decreased acetylcholine synthesis contributing to the cognitive decline observed in patients. In myasthenia gravis acetylcholine receptors are impaired but effective choline acetyltransferase activity is still necessary to manage neurotransmitter levels. A potential therapeutic angle involves targeting related proteins that exacerbate these conditions to support acetylcholine levels.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

See full target information CHAT

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2305326 PubMed38342616

2024

Botulinum Neurotoxin Induces Neurotoxic Microglia Mediated by Exogenous Inflammatory Responses.

Applications

Unspecified application

Species

Unspecified reactive species

Ghuncha Ambrin,You Jung Kang,Khanh Van Do,Charles Lee,Bal Ram Singh,Hansang Cho

Cell death & disease 14:741 PubMed37963881

2023

The long noncoding RNA nHOTAIRM1 is necessary for differentiation and activity of iPSC-derived spinal motor neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Paolo Tollis,Erika Vitiello,Francesco Migliaccio,Eleonora D'Ambra,Anna Rocchegiani,Maria Giovanna Garone,Irene Bozzoni,Alessandro Rosa,Annamaria Carissimo,Pietro Laneve,Elisa Caffarelli

International journal of molecular sciences 24: PubMed36835592

2023

The Biological Behaviors of Neural Stem Cell Affected by Microenvironment from Host Organotypic Brain Slices under Different Conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Jiao,Li Wang,Zhichao Zhang,Xinlin Chen,Haixia Lu,Yong Liu

Theranostics 13:1059-1075 PubMed36793868

2023

Hyperactive neuronal autophagy depletes BDNF and impairs adult hippocampal neurogenesis in a corticosterone-induced mouse model of depression.

Applications

Unspecified application

Species

Unspecified reactive species

Kuo Zhang,Fan Wang,Mengying Zhai,Meiyao He,Yuxuan Hu,Lijin Feng,Yuting Li,Jingyu Yang,Chunfu Wu

Stem cells international 2022:1320950 PubMed36530489

2022

The Efficiency of Direct Maturation: the Comparison of Two hiPSC Differentiation Approaches into Motor Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Catherine Schaefers,Simone Rothmiller,Horst Thiermann,Theo Rein,Annette Schmidt

Molecular psychiatry 26:443-455 PubMed33277628

2020

Genetically encoded sensors enable micro- and nano-scopic decoding of transmission in healthy and diseased brains.

Applications

Unspecified application

Species

Unspecified reactive species

Li Lin,Smriti Gupta,W Sharon Zheng,Ke Si,J Julius Zhu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Choline Acetyltransferase antibody

Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody (AB223346)

Immunocytochemistry - Anti-Choline Acetyltransferase antibody (AB223346)

Western blot - Anti-Choline Acetyltransferase antibody (AB223346)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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