AB254118

Anti-Choline Acetyltransferase antibody

Name: Anti-Choline Acetyltransferase antibody (ab254118) | Abcam
Brand: Abcam Limited
SKU: AB254118
Price: 530 USD
Availability: InStock

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(6 Publications)

Goat Polyclonal Choline Acetyltransferase antibody. Suitable for WB and reacts with Rat samples. Cited in 6 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human CHAT.

View Alternative Names

Choline O-acetyltransferase, CHOACTase, ChAT, Choline acetylase, CHAT

1 Images

Supplier Data

Western blot - Anti-Choline Acetyltransferase antibody (AB254118)

All lanes:

Western blot - Anti-Choline Acetyltransferase antibody (ab254118) at 1/100 dilution

All lanes:

Rat hippocampus lysate

Predicted band size: 82 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

applications

Immunogen

Native Full Length Protein corresponding to Human CHAT.

P28329

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Prepared from goat serum by affinity purification using a column to which the protein immunogen was coupled.

Storage buffer

Preservative: 0.2% Sodium azide Constituents: PBS, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Choline Acetyltransferase (ChAT) also known as EC 2.3.1.6 acts as the enzyme responsible for synthesizing the neurotransmitter acetylcholine. It catalyzes the transfer of an acetyl group from acetyl-CoA to choline to form acetylcholine. ChAT is a protein with an approximate mass of 70 kDa and is expressed in cholinergic neurons throughout the central and peripheral nervous systems. High expression is observed in regions such as the basal forebrain and neuromuscular junctions which are important for cholinergic signaling.

Biological function summary

The enzyme plays an important role in cholinergic conduction by producing acetylcholine which functions in synaptic transmission. It does not function as part of a larger complex but its activity directly influences cholinergic synapses. Choline acetyltransferase function ensures the synthesis and availability of acetylcholine for release into the synaptic cleft initiating muscle contraction and modulating activity in the central nervous system.

Pathways

Choline acetyltransferase is critical in pathways governing neurotransmitter release such as the cholinergic synapse pathway. It interacts functionally with other proteins within this pathway including acetylcholinesterase which breaks down acetylcholine in the synaptic cleft. This interaction ensures proper neurotransmitter regulation and recycling maintaining synapse function and communication within neuronal circuits.

Alterations in choline acetyltransferase activity relate to neurological disorders including Alzheimer's disease and myasthenia gravis. In Alzheimer's disease reduced choline acetyltransferase function leads to decreased acetylcholine synthesis contributing to the cognitive decline observed in patients. In myasthenia gravis acetylcholine receptors are impaired but effective choline acetyltransferase activity is still necessary to manage neurotransmitter levels. A potential therapeutic angle involves targeting related proteins that exacerbate these conditions to support acetylcholine levels.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

See full target information CHAT

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 29:e70732 PubMed40682486

2025

Ginsenoside Rb1 Alleviated the AKI to CKD Transition by Targeting VEGFR2.

Applications

Unspecified application

Species

Unspecified reactive species

Keying Zhang,Yuwei Ji,Zhangning Fu,Xiaochen Wang,Yifan Zhang,Yan Yang,Ran Liu,Xiangmei Chen,Guangyan Cai,Quan Hong

Proceedings of the National Academy of Sciences of the United States of America 121:e2410828121 PubMed39365823

2024

A mismatch between striatal cholinergic pauses and dopaminergic reward prediction errors.

Applications

Unspecified application

Species

Unspecified reactive species

Mariana Duhne,Ali Mohebi,Kyoungjun Kim,Lilian Pelattini,Joshua D Berke

CNS neuroscience & therapeutics 30:e14890 PubMed39097910

2024

Serotonin (5-HT) receptor agonist 2,5-dimethoxy-4-iodophenyl-2-aminopropane hydrochloride improves detrusor sphincter dyssynergia by inhibiting L-type voltage-gated calcium channels in spinal cord injured rats.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Lv,Mingzhuo Li,Xun Chen,Shengtian Li,Nailong Cao,Baojun Gu

Progress in neuro-psychopharmacology & biological psychiatry 125:110760 PubMed37031946

2023

Differential role of GABAergic and cholinergic ventral pallidal neurons in behavioral despair, conditioned fear memory and active coping.

Applications

Unspecified application

Species

Unspecified reactive species

Cemal Akmese,Cem Sevinc,Sahar Halim,Gunes Unal

eLife 11: PubMed36512397

2022

Spinal premotor interneurons controlling antagonistic muscles are spatially intermingled.

Applications

Unspecified application

Species

Unspecified reactive species

Remi Ronzano,Sophie Skarlatou,Bianca K Barriga,B Anne Bannatyne,Gardave Singh Bhumbra,Joshua D Foster,Jeffrey D Moore,Camille Lancelin,Amanda M Pocratsky,Mustafa Görkem Özyurt,Calvin Chad Smith,Andrew J Todd,David J Maxwell,Andrew J Murray,Samuel L Pfaff,Robert M Brownstone,Niccolò Zampieri,Marco Beato

Cell reports 38:110265 PubMed35045299

2022

Dopamine depletion selectively disrupts interactions between striatal neuron subtypes and LFP oscillations.

Applications

Unspecified application

Species

Unspecified reactive species

Dana Zemel,Howard Gritton,Cyrus Cheung,Sneha Shankar,Mark Kramer,Xue Han

View all publications

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Product promise

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Anti-Choline Acetyltransferase antibody

Western blot - Anti-Choline Acetyltransferase antibody (AB254118)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise