AB6168

Anti-Choline Acetyltransferase antibody

Name: Anti-Choline Acetyltransferase antibody (ab6168) | Abcam
Brand: Abcam Limited
SKU: AB6168
Price: 595 USD
Availability: InStock
Rating: 3 (7 reviews)

(7 Reviews)

(19 Publications)

Rabbit Polyclonal Choline Acetyltransferase antibody. Suitable for IHC-FrFl and reacts with Rat samples. Cited in 19 publications. Immunogen corresponding to Synthetic Peptide within Human CHAT aa 150-200 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Choline O-acetyltransferase, CHOACTase, ChAT, Choline acetylase, CHAT

1 Images

Immunohistochemistry - Free Floating - Anti-Choline Acetyltransferase antibody (AB6168)

IHC-FrFl

Supplier Data

Immunohistochemistry - Free Floating - Anti-Choline Acetyltransferase antibody (AB6168)

Immunohistochemistry analysis of free-floating rat forebrain tissue labelling Choline Acetyltransferase with ab6168 at a dilution of 1/100.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

IHC-FrFl

applications

Immunogen

Synthetic Peptide within Human CHAT aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P28329

Specificity

Extensive immunohistochemistry indicates localisation to cholinergic nervous system only. Will stain cholinergic neurons in rat central and peripheral nervous systems.

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Reactivity data

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Product details

This antibody appears to have identical characteristics to those described by Benecke et al (1993). The antigen is the same. It is an excellent reagent for immunohistochemistry for both peripheral and central nervous system, staining both cell bodies and nerve terminals.

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Add glycerol to a final volume of 50% for extra stability and aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Choline Acetyltransferase (ChAT) also known as EC 2.3.1.6 acts as the enzyme responsible for synthesizing the neurotransmitter acetylcholine. It catalyzes the transfer of an acetyl group from acetyl-CoA to choline to form acetylcholine. ChAT is a protein with an approximate mass of 70 kDa and is expressed in cholinergic neurons throughout the central and peripheral nervous systems. High expression is observed in regions such as the basal forebrain and neuromuscular junctions which are important for cholinergic signaling.

Biological function summary

The enzyme plays an important role in cholinergic conduction by producing acetylcholine which functions in synaptic transmission. It does not function as part of a larger complex but its activity directly influences cholinergic synapses. Choline acetyltransferase function ensures the synthesis and availability of acetylcholine for release into the synaptic cleft initiating muscle contraction and modulating activity in the central nervous system.

Pathways

Choline acetyltransferase is critical in pathways governing neurotransmitter release such as the cholinergic synapse pathway. It interacts functionally with other proteins within this pathway including acetylcholinesterase which breaks down acetylcholine in the synaptic cleft. This interaction ensures proper neurotransmitter regulation and recycling maintaining synapse function and communication within neuronal circuits.

Alterations in choline acetyltransferase activity relate to neurological disorders including Alzheimer's disease and myasthenia gravis. In Alzheimer's disease reduced choline acetyltransferase function leads to decreased acetylcholine synthesis contributing to the cognitive decline observed in patients. In myasthenia gravis acetylcholine receptors are impaired but effective choline acetyltransferase activity is still necessary to manage neurotransmitter levels. A potential therapeutic angle involves targeting related proteins that exacerbate these conditions to support acetylcholine levels.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

See full target information CHAT

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Nature structural & molecular biology 32:1932-1946 PubMed40646310

2025

Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control.

Applications

Unspecified application

Species

Unspecified reactive species

Geraldine Rodschinka,Sergio Forcelloni,Felix M Kühner,Sascha Wani,Henrick Riemenschneider,Dieter Edbauer,Andrew Behrens,Danny D Nedialkova

Nature cell biology 26:100-112 PubMed38191669

2024

Selective gene expression maintains human tRNA anticodon pools during differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Lexi Gao,Andrew Behrens,Geraldine Rodschinka,Sergio Forcelloni,Sascha Wani,Katrin Strasser,Danny D Nedialkova

Neuron 111:1914-1932.e6 PubMed37084721

2023

Bidirectional control of parathyroid hormone and bone mass by subfornical organ.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Zhang,Nian Liu,Jie Shao,Dashuang Gao,Yunhui Liu,Yingzi Zhao,Chuanliang Han,Di Chen,Liping Wang,William Weijia Lu,Fan Yang

Frontiers in molecular neuroscience 16:1158525 PubMed37152428

2023

Medial and dorsal lateral septum involving social disruption stress-primed escalation in acid-induced writhes.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Han Liao,Li-Han Sun,Yi-Chi Su,Wei-Jen Yao,Lung Yu

Neural regeneration research 18:922-928 PubMed36204864

2022

Necroptosis plays a crucial role in the exacerbation of retinal injury after blunt ocular trauma.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Huan,Xiu-Quan Wu,Tao Chen,Ya-Nan Dou,Bo Jia,Xin He,Dong-Yu Wei,Zhou Fei,Fei Fei

Frontiers in aging neuroscience 13:770948 PubMed35185516

2022

Electro-Acupuncture Improve the Early Pattern Separation in Alzheimer's Disease Mice Basal Forebrain-Hippocampus Cholinergic Neural Circuit.

Applications

Unspecified application

Species

Unspecified reactive species

Long Li,Jianhong Li,Yaling Dai,Minguang Yang,Shengxiang Liang,Zhifu Wang,Weilin Liu,Lidian Chen,Jing Tao

Computational and mathematical methods in medicine 2021:8522417 PubMed35003326

2021

Activation of Neural Modeling-Related Genes in the Heart of Mice after Gamma Irradiation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyong Liu,Jing Liu,Dan Hu,Juanjuan Du,Donglu Liu,Ximin Wang,Jiandong Zhang,Yinglong Hou

Molecular psychiatry : PubMed32555286

2020

A new GABAergic somatostatin projection from the BNST onto accumbal parvalbumin neurons controls anxiety.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Xiao,Xinyi Zhou,Pengfei Wei,Li Xie,Yaning Han,Jie Wang,Aoling Cai,Fuqiang Xu,Jie Tu,Liping Wang

Frontiers in cellular neuroscience 14:38 PubMed32265654

2020

Time-Course Changes and Role of Autophagy in Primary Spinal Motor Neurons Subjected to Oxygen-Glucose Deprivation: Insights Into Autophagy Changes in a Cellular Model of Spinal Cord Ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Shudong Chen,Ruimin Tian,Dan Luo,Zhifeng Xiao,Hui Li,Dingkun Lin

Molecular medicine reports 21:1172-1180 PubMed31922229

2020

Neural stem cell transplantation improves learning and memory by protecting cholinergic neurons and restoring synaptic impairment in an amyloid precursor protein/presenilin 1 transgenic mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Zhu,Nianping Zhang,Nan Hu,Rongrong Jiang,Huicong Lu,Aiguo Xuan,Dahong Long,Yan Chen

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Choline Acetyltransferase antibody

Immunohistochemistry - Free Floating - Anti-Choline Acetyltransferase antibody (AB6168)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purity

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

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