Rabbit Polyclonal Choline Acetyltransferase antibody. Suitable for IHC-FrFl and reacts with Rat samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human CHAT aa 150-200 conjugated to Keyhole Limpet Haemocyanin.
Images
Publications
Filter by
- Frontiers in molecular neuroscience 16:11585252023Medial and dorsal lateral septum involving social disruption stress-primed escalation in acid-induced writhes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Han Liao,Li-Han Sun,Yi-Chi Su,Wei-Jen Yao,Lung YuPubMed 37152428
- Neural regeneration research 18:922-9282022Necroptosis plays a crucial role in the exacerbation of retinal injury after blunt ocular trauma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Huan,Xiu-Quan Wu,Tao Chen,Ya-Nan Dou,Bo Jia,Xin He,Dong-Yu Wei,Zhou Fei,Fei FeiPubMed 36204864
- Frontiers in aging neuroscience 13:7709482022Electro-Acupuncture Improve the Early Pattern Separation in Alzheimer's Disease Mice Basal Forebrain-Hippocampus Cholinergic Neural Circuit.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLong Li,Jianhong Li,Yaling Dai,Minguang Yang,Shengxiang Liang,Zhifu Wang,Weilin Liu,Lidian Chen,Jing TaoPubMed 35185516
- Computational and mathematical methods in medicine 2021:85224172021Activation of Neural Modeling-Related Genes in the Heart of Mice after Gamma Irradiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiyong Liu,Jing Liu,Dan Hu,Juanjuan Du,Donglu Liu,Ximin Wang,Jiandong Zhang,Yinglong HouPubMed 35003326
- Molecular psychiatry :2020A new GABAergic somatostatin projection from the BNST onto accumbal parvalbumin neurons controls anxiety.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Xiao et. al.PubMed 32555286
- Molecular medicine reports 21:1172-11802020Neural stem cell transplantation improves learning and memory by protecting cholinergic neurons and restoring synaptic impairment in an amyloid precursor protein/presenilin 1 transgenic mouse model of Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQing Zhu et. al.PubMed 31922229
- Brain : a journal of neurology 143:69-932019ZPR1 prevents R-loop accumulation, upregulates SMN2 expression and rescues spinal muscular atrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnnapoorna Kannan,Xiaoting Jiang,Lan He,Saif Ahmad,Laxman GangwaniPubMed 31828288
- Theranostics 9:4241-42542019A Combination of Oxo-M and 4-PPBP as a potential regenerative therapeutics for tendon injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSolaiman Tarafder et. al.PubMed 31281545
- Advanced healthcare materials 7:e18003152018Functional Multichannel Poly(Propylene Fumarate)-Collagen Scaffold with Collagen-Binding Neurotrophic Factor 3 Promotes Neural Regeneration After Transected Spinal Cord Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXi Chen et. al.PubMed 29920990
- Journal of chemical neuroanatomy 92:35-402018Differentiation of neural crest stem cells from nasal mucosa into motor neuron-like cells.Applications:ICC/IFReactive species:Unspecified reactive speciesZohreh Bagher,Seyed Kamran Kamrava,Rafieh Alizadeh,Mohammad Farhadi,Moloud Absalan,Masoumeh Falah,Faezeh Faghihi,Arash Zare-Sadeghi,Ali KomeiliPubMed 29807106
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CHAT aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P28329
Consider this alternative
Reactivity data
Select an application| IHC-FrFl | |
|---|---|
| Human | Not recommended |
| Rat | Tested |
| Chicken | Not recommended |
| Pig | Not recommended |
| Primates | Not recommended |
| Sheep | Not recommended |
IHC-FrFl
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Chicken, Human, Pig, Primates | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Alternative names
Choline O-acetyltransferase, CHOACTase, ChAT, Choline acetylase, CHAT
Recommended products
Rabbit Polyclonal Choline Acetyltransferase antibody. Suitable for IHC-FrFl and reacts with Rat samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human CHAT aa 150-200 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CHAT aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P28329
- Purity
- Whole antiserum
- Specificity
Extensive immunohistochemistry indicates localisation to cholinergic nervous system only. Will stain cholinergic neurons in rat central and peripheral nervous systems.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Add glycerol to a final volume of 50% for extra stability and aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This antibody appears to have identical characteristics to those described by Benecke et al (1993). The antigen is the same. It is an excellent reagent for immunohistochemistry for both peripheral and central nervous system, staining both cell bodies and nerve terminals.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Choline Acetyltransferase (ChAT) also known as EC 2.3.1.6 acts as the enzyme responsible for synthesizing the neurotransmitter acetylcholine. It catalyzes the transfer of an acetyl group from acetyl-CoA to choline to form acetylcholine. ChAT is a protein with an approximate mass of 70 kDa and is expressed in cholinergic neurons throughout the central and peripheral nervous systems. High expression is observed in regions such as the basal forebrain and neuromuscular junctions which are important for cholinergic signaling.
- Biological function summary
The enzyme plays an important role in cholinergic conduction by producing acetylcholine which functions in synaptic transmission. It does not function as part of a larger complex but its activity directly influences cholinergic synapses. Choline acetyltransferase function ensures the synthesis and availability of acetylcholine for release into the synaptic cleft initiating muscle contraction and modulating activity in the central nervous system.
- Pathways
Choline acetyltransferase is critical in pathways governing neurotransmitter release such as the cholinergic synapse pathway. It interacts functionally with other proteins within this pathway including acetylcholinesterase which breaks down acetylcholine in the synaptic cleft. This interaction ensures proper neurotransmitter regulation and recycling maintaining synapse function and communication within neuronal circuits.
- Associated diseases and disorders
Alterations in choline acetyltransferase activity relate to neurological disorders including Alzheimer's disease and myasthenia gravis. In Alzheimer's disease reduced choline acetyltransferase function leads to decreased acetylcholine synthesis contributing to the cognitive decline observed in patients. In myasthenia gravis acetylcholine receptors are impaired but effective choline acetyltransferase activity is still necessary to manage neurotransmitter levels. A potential therapeutic angle involves targeting related proteins that exacerbate these conditions to support acetylcholine levels.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Immunohistochemistry - Free Floating - Anti-Choline Acetyltransferase antibody (ab6168)
Immunohistochemistry analysis of free-floating rat forebrain tissue labelling Choline Acetyltransferase with ab6168 at a dilution of 1/100.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com