Rabbit Recombinant Monoclonal Choline Acetyltransferase antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (AB224267), expandable thumbnail](https://content.abcam.com/products/images/choline-acetyltransferase-antibody-epr13024b-bsa-and-azide-free-ab224267--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img59835.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (AB224267)")
![Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (AB224267), expandable thumbnail](https://content.abcam.com/products/images/choline-acetyltransferase-antibody-epr13024b-bsa-and-azide-free-ab224267--immunocytochemistry-immunofluorescence-img19741.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (AB224267)")
![Immunoprecipitation - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (AB224267), expandable thumbnail](https://content.abcam.com/products/images/choline-acetyltransferase-antibody-epr13024b-bsa-and-azide-free-ab224267--immunoprecipitation-img158562.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (AB224267)")
![Flow Cytometry (Intracellular) - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (AB224267), expandable thumbnail](https://content.abcam.com/products/images/choline-acetyltransferase-antibody-epr13024b-bsa-and-azide-free-ab224267--flow-cytometry-intracellular-img6324.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (AB224267)")
![Immunoprecipitation - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (AB224267), expandable thumbnail](https://content.abcam.com/products/images/choline-acetyltransferase-antibody-epr13024b-bsa-and-azide-free-ab224267--immunoprecipitation-img10039.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (AB224267)")
Publications
Filter by
- Molecular medicine (Cambridge, Mass.) 23:34-392017Deficiency of α7 nicotinic acetylcholine receptor attenuates bleomycin-induced lung fibrosis in mice.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected | Expected | Expected | Expected |
| Rat | Expected | Tested | Expected | Expected | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes For unpurified format use at 1/100 dilution. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
7 products for Alternative Version
Target data
Function
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Alternative names
Choline O-acetyltransferase, CHOACTase, ChAT, Choline acetylase, CHAT
Recommended products
Rabbit Recombinant Monoclonal Choline Acetyltransferase antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR13024(B)
- Purification technique
- Affinity purification Protein A
- Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P, Flow Cyt (Intra), IHC-Fr, ICC/IF and IP for mouse and rat.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab224267 is the carrier-free version of Anti-Choline Acetyltransferase antibody [EPR13024(B)] ab181023.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Choline Acetyltransferase (ChAT) also known as EC 2.3.1.6 acts as the enzyme responsible for synthesizing the neurotransmitter acetylcholine. It catalyzes the transfer of an acetyl group from acetyl-CoA to choline to form acetylcholine. ChAT is a protein with an approximate mass of 70 kDa and is expressed in cholinergic neurons throughout the central and peripheral nervous systems. High expression is observed in regions such as the basal forebrain and neuromuscular junctions which are important for cholinergic signaling.
- Biological function summary
The enzyme plays an important role in cholinergic conduction by producing acetylcholine which functions in synaptic transmission. It does not function as part of a larger complex but its activity directly influences cholinergic synapses. Choline acetyltransferase function ensures the synthesis and availability of acetylcholine for release into the synaptic cleft initiating muscle contraction and modulating activity in the central nervous system.
- Pathways
Choline acetyltransferase is critical in pathways governing neurotransmitter release such as the cholinergic synapse pathway. It interacts functionally with other proteins within this pathway including acetylcholinesterase which breaks down acetylcholine in the synaptic cleft. This interaction ensures proper neurotransmitter regulation and recycling maintaining synapse function and communication within neuronal circuits.
- Associated diseases and disorders
Alterations in choline acetyltransferase activity relate to neurological disorders including Alzheimer's disease and myasthenia gravis. In Alzheimer's disease reduced choline acetyltransferase function leads to decreased acetylcholine synthesis contributing to the cognitive decline observed in patients. In myasthenia gravis acetylcholine receptors are impaired but effective choline acetyltransferase activity is still necessary to manage neurotransmitter levels. A potential therapeutic angle involves targeting related proteins that exacerbate these conditions to support acetylcholine levels.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267), expandable thumbnail](https://content.abcam.com/products/images/choline-acetyltransferase-antibody-epr13024b-bsa-and-azide-free-ab224267--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img59835.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebrum tissue sections labeling Choline Acetyltransferase with purified Anti-Choline Acetyltransferase antibody [EPR13024(B)] ab181023 at 1/2000 dilution (0.28 μg/ml). Heat mediated antigen retrieval was performed using heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Choline Acetyltransferase antibody [EPR13024(B)] ab181023).
![Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267), expandable thumbnail](https://content.abcam.com/products/images/choline-acetyltransferase-antibody-epr13024b-bsa-and-azide-free-ab224267--immunocytochemistry-immunofluorescence-img19741.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)")
Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)
Immunocytochemistry/ Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell) cells labeling Choline Acetyltransferase with purified Anti-Choline Acetyltransferase antibody [EPR13024(B)] ab181023 at 1/50 dilution (10 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Choline Acetyltransferase antibody [EPR13024(B)] ab181023).
![Immunoprecipitation - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267), expandable thumbnail](https://content.abcam.com/products/images/choline-acetyltransferase-antibody-epr13024b-bsa-and-azide-free-ab224267--immunoprecipitation-img158562.jpg "Immunoprecipitation - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)")
Immunoprecipitation - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)
Anti-Choline Acetyltransferase antibody [EPR13024(B)] ab181023 (purified) at 1/30 dilution (2ug) immunoprecipitating Choline Acetyltransferase in Human fetal brain lysate. Human fetal brain lysate 10ug
Lane 2 (+): Anti-Choline Acetyltransferase antibody [EPR13024(B)] ab181023 & Human fetal brain lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Choline Acetyltransferase antibody [EPR13024(B)] ab181023 in Human fetal brain lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.Blocking and diluting buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Choline Acetyltransferase antibody [EPR13024(B)] ab181023).
All lanes: Immunoprecipitation - Anti-Choline Acetyltransferase antibody [EPR13024(B)] (Anti-Choline Acetyltransferase antibody [EPR13024(B)] ab181023)
Predicted band size: 82 kDa
![Flow Cytometry (Intracellular) - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267), expandable thumbnail](https://content.abcam.com/products/images/choline-acetyltransferase-antibody-epr13024b-bsa-and-azide-free-ab224267--flow-cytometry-intracellular-img6324.jpg "Flow Cytometry (Intracellular) - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)")
Flow Cytometry (Intracellular) - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)
Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Choline Acetyltransferase with purified Anti-Choline Acetyltransferase antibody [EPR13024(B)] ab181023 at 1/60 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Choline Acetyltransferase antibody [EPR13024(B)] ab181023).
![Immunoprecipitation - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267), expandable thumbnail](https://content.abcam.com/products/images/choline-acetyltransferase-antibody-epr13024b-bsa-and-azide-free-ab224267--immunoprecipitation-img10039.jpg "Immunoprecipitation - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)")
Immunoprecipitation - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)
Lysate from Human fetal brain (Lane 1) and negative control (Lane 2) were immunoprecipitated with Anti-Choline Acetyltransferase antibody [EPR13024(B)] ab181023 (unpurified) at a 1/70 dilution. A anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Choline Acetyltransferase antibody [EPR13024(B)] ab181023).
All lanes: Immunoprecipitation - Anti-Choline Acetyltransferase antibody [EPR13024(B)] (Anti-Choline Acetyltransferase antibody [EPR13024(B)] ab181023)
Predicted band size: 82 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267), expandable thumbnail](https://content.abcam.com/products/images/choline-acetyltransferase-antibody-epr13024b-bsa-and-azide-free-ab224267--immunocytochemistry-immunofluorescence-img1652.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)")
Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)
This ICC data was generated using the same anti-Choline Acetyltransferase antibody clone [EPR12024(B)] in a different buffer formulation (cat# Anti-Choline Acetyltransferase antibody [EPR13024(B)] ab181023).
Immunofluorescent analysis of 4% paraformaldehyde fixed SH-SY5Y cells labeling Choline Acetyltransferase using Anti-Choline Acetyltransferase antibody [EPR13024(B)] ab181023 (unpurified) at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/100 dilution.
![Flow Cytometry (Intracellular) - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267), expandable thumbnail](https://content.abcam.com/products/images/choline-acetyltransferase-antibody-epr13024b-bsa-and-azide-free-ab224267--flow-cytometry-intracellular-img79646.jpg "Flow Cytometry (Intracellular) - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)")
Flow Cytometry (Intracellular) - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)
Intracellular Flow Cytometry analysis of SH-SY5Y cells labeling Choline Acetyltransferase using Anti-Choline Acetyltransferase antibody [EPR13024(B)] ab181023 (unpurified) at a 1/110 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Choline Acetyltransferase antibody [EPR13024(B)] ab181023).
![Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267), expandable thumbnail](https://content.abcam.com/products/images/choline-acetyltransferase-antibody-epr13024b-bsa-and-azide-free-ab224267--immunocytochemistry-immunofluorescence-img1652.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)")
![Flow Cytometry (Intracellular) - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267), expandable thumbnail](https://content.abcam.com/products/images/choline-acetyltransferase-antibody-epr13024b-bsa-and-azide-free-ab224267--flow-cytometry-intracellular-img79646.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)")
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