Anti-Choline Acetyltransferase antibody [EPR16590] ab178850 is a rabbit monoclonal antibody that is used in Choline Acetyltransferase western blotting and IHC. Suitable for mouse and rat samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR16590 is the most widely used clone for Choline Acetyltransferase on the market and is cited in >60 publications
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
New 20 ul size available
IgG
Rabbit
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | IHC-P | |
---|---|---|
Mouse | Tested | Tested |
Rat | Tested | Expected |
Guinea pig | Tested | Tested |
Rabbit | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Guinea pig | Dilution info 1/1000 | Notes - |
Species Rabbit | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/2000 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Guinea pig | Dilution info 1/2000 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Rabbit | Dilution info Use at an assay dependent concentration. | Notes - |
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Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Choline O-acetyltransferase, CHOACTase, ChAT, Choline acetylase, Chat
Anti-Choline Acetyltransferase antibody [EPR16590] ab178850 is a rabbit monoclonal antibody that is used in Choline Acetyltransferase western blotting and IHC. Suitable for mouse and rat samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR16590 is the most widely used clone for Choline Acetyltransferase on the market and is cited in >60 publications
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
New 20 ul size available
IgG
Rabbit
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR16590
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
Choline Acetyltransferase (ChAT) also known as EC 2.3.1.6 acts as the enzyme responsible for synthesizing the neurotransmitter acetylcholine. It catalyzes the transfer of an acetyl group from acetyl-CoA to choline to form acetylcholine. ChAT is a protein with an approximate mass of 70 kDa and is expressed in cholinergic neurons throughout the central and peripheral nervous systems. High expression is observed in regions such as the basal forebrain and neuromuscular junctions which are important for cholinergic signaling.
The enzyme plays an important role in cholinergic conduction by producing acetylcholine which functions in synaptic transmission. It does not function as part of a larger complex but its activity directly influences cholinergic synapses. Choline acetyltransferase function ensures the synthesis and availability of acetylcholine for release into the synaptic cleft initiating muscle contraction and modulating activity in the central nervous system.
Choline acetyltransferase is critical in pathways governing neurotransmitter release such as the cholinergic synapse pathway. It interacts functionally with other proteins within this pathway including acetylcholinesterase which breaks down acetylcholine in the synaptic cleft. This interaction ensures proper neurotransmitter regulation and recycling maintaining synapse function and communication within neuronal circuits.
Alterations in choline acetyltransferase activity relate to neurological disorders including Alzheimer's disease and myasthenia gravis. In Alzheimer's disease reduced choline acetyltransferase function leads to decreased acetylcholine synthesis contributing to the cognitive decline observed in patients. In myasthenia gravis acetylcholine receptors are impaired but effective choline acetyltransferase activity is still necessary to manage neurotransmitter levels. A potential therapeutic angle involves targeting related proteins that exacerbate these conditions to support acetylcholine levels.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Terms & Conditions.
Immunohistochemical analysis of paraffin-embedded Guinea Pig cerebral cortex tissue labeling Choline Acetyltransferase with ab178850 at 1/2000 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasm staining on neurons of Guinea Pig cerebral cortex is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Choline Acetyltransferase with ab178850 at 1/2000 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasm staining on neurons of Mouse cerebral cortex is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/5000 dilution
Lane 1: Guinea Pig brain lysate at 20 µg
Lane 2: Guinea Pig cerebellum lysate at 20 µg
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 82 kDa
Observed band size: 72 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue sections labeling Choline Acetyltransferase with ab178850 at 1/2000 dilution. Cells were fixed in 10% normal buffered formalin, heat mediated antigen retrieval was performed using TE pH9. A donkey anti-rabbit Alexa Fluor 647 was used as the secondary antibody at 1/300 dilution. Green was a false colour used to represent the Alexa Fluor 647 fluorescent signal.
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/1000 dilution
All lanes: Rabbit brain lysate at 10 µg
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 82 kDa
Observed band size: 72 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/1000 dilution
All lanes: Rat brain lysate at 10 µg
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 82 kDa
Observed band size: 72 kDa
All lanes: Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/2000 dilution
All lanes: Mouse brain tissue lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 82 kDa
Observed band size: 72 kDa
Exposure time: 3min
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