AB178850

Anti-Choline Acetyltransferase antibody [EPR16590]

Name: Anti-choline acetyltransferase antibody [EPR16590] (ab178850) | Abcam
Brand: Abcam Limited
SKU: AB178850
Price: 220 USD
Availability: InStock
Rating: 5 (6 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(6 Reviews)

(80 Publications)

Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) is a rabbit monoclonal antibody detecting Choline Acetyltransferase in Western Blot, IHC-P. Suitable for Guinea pig, Mouse, Rabbit, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 50 publications

View Alternative Names

Choline O-acetyltransferase, CHOACTase, ChAT, Choline acetylase, Chat

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)

Immunohistochemical analysis of paraffin-embedded Guinea Pig cerebral cortex tissue labeling Choline Acetyltransferase with ab178850 at 1/2000 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasm staining on neurons of Guinea Pig cerebral cortex is observed. Counter stained with Hematoxylin.

Negative control : Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Choline Acetyltransferase with ab178850 at 1/2000 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasm staining on neurons of Mouse cerebral cortex is observed. Counter stained with Hematoxylin.

Negative control : Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

AbReview56980****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue sections labeling Choline Acetyltransferase with ab178850 at 1/2000 dilution. Cells were fixed in 10% normal buffered formalin, heat mediated antigen retrieval was performed using TE pH9. A donkey anti-rabbit Alexa Fluor 647 was used as the secondary antibody at 1/300 dilution. Green was a false colour used to represent the Alexa Fluor 647 fluorescent signal.

This image is courtesy of an anonymous abreview.

Supplier Data

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/2000 dilution

All lanes:

Mouse brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 82 kDa

Observed band size: 72 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/1000 dilution

All lanes:

Rat brain lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 82 kDa

Observed band size: 72 kDa

false

Supplier Data

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/1000 dilution

All lanes:

Rabbit brain lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 82 kDa

Observed band size: 72 kDa

false

Supplier Data

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/5000 dilution

Lane 1:

Guinea Pig brain lysate at 20 µg

Lane 2:

Guinea Pig cerebellum lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 82 kDa

Observed band size: 72 kDa

false

Carrier free

Anti-Choline Acetyltransferase antibody [EPR16590] - BSA and Azide free
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Choline Acetyltransferase antibody [EPR16590]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Choline Acetyltransferase antibody [EPR16590]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Choline Acetyltransferase antibody [EPR16590]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Choline Acetyltransferase antibody [EPR16590]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Choline Acetyltransferase antibody [EPR16590]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Choline Acetyltransferase antibody [EPR16590]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16590

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Rabbit, Guinea pig

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rabbit": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Product Specifications

Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-P, WB in guinea pig, mouse, rabbit, rat samples.
Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) specifically detects Choline Acetyltransferase (UniProt ID: P13222; Molecular weight: 72kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).

Quality and Validation

Abcam's high quality manufacturing and validation processes ensure Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) has been cited over 54 times in peer reviewed journals and is trusted by the scientific community.
Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) has 5 independent reviews from customers.

Related Products
Conjugation-ready, carrier free format available for antibody clone EPR16590 - ab221793.
Antibody clone EPR16590 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, Alexa Fluor® 488, Alexa Fluor® 594, Alexa Fluor® 568, Alexa Fluor® 555, Alexa Fluor® 750 (ab225262, ab225263, ab311819, ab313102, ab313295, ab321220).
Target Information
Choline Acetyltransferase (ChAT) is an enzyme crucial for the synthesis of acetylcholine, a neurotransmitter involved in many functions, including muscle activation and cognitive processes. It serves as a marker for cholinergic neurons in research. Impaired ChAT activity is implicated in various conditions, including Alzheimer's disease (AD), where decreased acetylcholine levels lead to cognitive deficits, and myasthenia gravis, an autoimmune disorder that disrupts neuromuscular communication.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Choline Acetyltransferase (ChAT) also known as EC 2.3.1.6 acts as the enzyme responsible for synthesizing the neurotransmitter acetylcholine. It catalyzes the transfer of an acetyl group from acetyl-CoA to choline to form acetylcholine. ChAT is a protein with an approximate mass of 70 kDa and is expressed in cholinergic neurons throughout the central and peripheral nervous systems. High expression is observed in regions such as the basal forebrain and neuromuscular junctions which are important for cholinergic signaling.

Biological function summary

The enzyme plays an important role in cholinergic conduction by producing acetylcholine which functions in synaptic transmission. It does not function as part of a larger complex but its activity directly influences cholinergic synapses. Choline acetyltransferase function ensures the synthesis and availability of acetylcholine for release into the synaptic cleft initiating muscle contraction and modulating activity in the central nervous system.

Pathways

Choline acetyltransferase is critical in pathways governing neurotransmitter release such as the cholinergic synapse pathway. It interacts functionally with other proteins within this pathway including acetylcholinesterase which breaks down acetylcholine in the synaptic cleft. This interaction ensures proper neurotransmitter regulation and recycling maintaining synapse function and communication within neuronal circuits.

Alterations in choline acetyltransferase activity relate to neurological disorders including Alzheimer's disease and myasthenia gravis. In Alzheimer's disease reduced choline acetyltransferase function leads to decreased acetylcholine synthesis contributing to the cognitive decline observed in patients. In myasthenia gravis acetylcholine receptors are impaired but effective choline acetyltransferase activity is still necessary to manage neurotransmitter levels. A potential therapeutic angle involves targeting related proteins that exacerbate these conditions to support acetylcholine levels.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

See full target information Chat

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Publications (80)

Recent publications for all applications. Explore the full list and refine your search

World journal of diabetes 16:107779 PubMed40837348

2025

Electroacupuncture with different current intensities can improve gastrointestinal motility in diabetic gastroparesis vagal and sympathetic pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Wen Tang,You Zhang,Jin Zhou,Yu-Ting Peng,Yan Zi,Yan-Rong Wei,Zeng-Hui Yue

Nature communications 16:5987 PubMed40593720

2025

A rationally designed 2C inhibitor prevents enterovirus D68-infected mice from developing paralysis.

Applications

Unspecified application

Species

Unspecified reactive species

Kan Li,Michael J Rudy,Yanmei Hu,Haozhou Tan,George Lambrinidis,Xiangmeng Wu,Kyriakos Georgiou,Bin Tan,Joshua Frost,Courtney Wilson,Penny Clarke,Antonios Kolocouris,Qing-Yu Zhang,Kenneth L Tyler,Jun Wang

Nature communications 16:5215 PubMed40473608

2025

A gatekeeper sympathetic control of lacrimal tear secretion and dry eye onset through the NA-Adra1a-Ucp2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mingli Qu,Qun Wang,Xiaofei Bai,Jing Feng,Sai Zhang,Yangyang Zhang,Qing Chen,Hai Zhu,Hengrui Zhang,Qunqin Guo,Bin Zhang,Shengqian Dou,Yujie Qiao,Hongwei Wang,Yihai Cao,Lixin Xie,Qingjun Zhou

Frontiers in aging neuroscience 17:1567650 PubMed40416734

2025

Effects of cannabidiol (CBD) treatment on age-related cognitive decline in C57 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Behroo Mirza Agha,Merrin Monteith,Jarem Earl,Keona Ganske,Tina Kaloa,Kelan J McDonald,Abigail G Nixon,Maleeha Panjwani,Danika Robinson,Valeria Rusnak,Majid H Mohajerani,Igor Kovalchuk,Robert J Sutherland,Nancy S Hong,Robert J McDonald

Molecular medicine (Cambridge, Mass.) 31:158 PubMed40301740

2025

Preclinical evaluation of AAV9-coSMN1 gene therapy for spinal muscular atrophy: efficacy and safety in mouse models and non-human primates.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhao Ma,Zhijie Wu,Tianyi Zhao,Yan Xia,Jing Qin,Xue Tian,Xin Li,Jun He,Yan Zhang,Lina Zhang,Li Li,Zheyue Dong,Zhichun Feng,Xiaoyan Dong,Wang Sheng,Xiaobing Wu

Pharmaceutical biology 63:218-228 PubMed40205764

2025

, a standardized herbal formula, promotes gastrointestinal motility via modulation of the acetylcholine pathway in a loperamide-induced functional dyspepsia mice.

Applications

Unspecified application

Species

Unspecified reactive species

Seung-Ju Hwang,Chang-Seob Seo,Dong-Cheol Baek,Tae-Wook Woo,Jing-Hua Wang,Jin-Seok Lee,Yu-Jin Choi,Ji-Yeon Gu,Dong-Seon Kim,Chang-Gue Son

Neuropathology and applied neurobiology 51:e70016 PubMed40192272

2025

Intramuscular Nerve Bundles Reflect TDP-43 Pathology in the Medulla and Spinal Cord of ALS Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Kurashige,Tomomi Murao,Yuhei Kanaya,Yoriko Dodo,Tomohito Sugiura,Kazuya Kuraoka,Tomohiko Ohshita

mBio 16:e0390624 PubMed40126012

2025

EV68-228-N monoclonal antibody treatment halts progression of paralysis in a mouse model of EV-D68 induced acute flaccid myelitis.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Rudy,Courtney J Wilson,Brendan Hinckley,Danielle C Baker,Joshua M Royal,Marshall P Hoke,Miles B Brennan,Matthew R Vogt,Penny Clarke,Kenneth L Tyler

iScience 28:111953 PubMed40083716

2025

KAT6B overexpression in mice causes aggression, anxiety, and epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Maria I Bergamasco,Ezgi Ozturk,Pablo M Casillas-Espinosa,Alexandra L Garnham,Waruni Abeysekera,Verena C Wimmer,Pradeep Rajasekhar,Hannah K Vanyai,Lachlan Whitehead,Marnie E Blewitt,Kelly Rogers,Adam P Vogel,Anthony J Hannan,Gordon K Smyth,Nigel C Jones,Tim Thomas,Anne K Voss

International journal of medical sciences 22:775-789 PubMed39991760

2025

Intramuscular Injection of rAAV2-retro for Low Motor Neuron Transduction: Evaluating Five Promoters.

Applications

Unspecified application

Species

Unspecified reactive species

Xueqi Gong,Haitong Gao,Wenyuan Wang,Tonghui Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Choline Acetyltransferase antibody [EPR16590]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)

Western blot - Anti-Choline Acetyltransferase antibody [EPR16590] (AB178850)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (80)

Product promise