Rabbit Recombinant Monoclonal Choline Acetyltransferase antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Guinea pig, Rabbit samples. Cited in 2 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] - BSA and Azide free (AB221793), expandable thumbnail](https://content.abcam.com/products/images/choline-acetyltransferase-antibody-epr16590-bsa-and-azide-free-ab221793--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img70184.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] - BSA and Azide free (AB221793)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] - BSA and Azide free (AB221793), expandable thumbnail](https://content.abcam.com/products/images/choline-acetyltransferase-antibody-epr16590-bsa-and-azide-free-ab221793--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img38102.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] - BSA and Azide free (AB221793)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] - BSA and Azide free (AB221793), expandable thumbnail](https://content.abcam.com/products/images/choline-acetyltransferase-antibody-epr16590-bsa-and-azide-free-ab221793--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134088.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] - BSA and Azide free (AB221793)")
Publications
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- Journal of neuroinflammation 20:1582023Caudal DMN neurons innervate the spleen and release CART peptide to regulate neuroimmune function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNobuhide Kobori,Anthony N Moore,John B Redell,Pramod K DashPubMed 37403174
- The European journal of neuroscience 56:3755-37782022P38 initiates degeneration of midbrain GABAergic and glutamatergic neurons in diabetes models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAisan Farhadi,Mehdi Totonchi,Seyed Masood Nabavi,Hossein Baharvand,Hossein Pakdaman,Ensiyeh Hajizadeh-Saffar,Seyed Ahmad Mousavi,Fatemeh Hadi,Hamed Al-Sinawi,Quan Li,Jin-San Zhang,Yaser Tahamtani,Koorosh ShahpasandPubMed 35513862
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Mouse | Expected | Predicted |
| Rat | Expected | Predicted |
| Guinea pig | Expected | Tested |
| Rabbit | Expected | Predicted |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Guinea pig | Dilution info - | Notes - |
Species Rabbit | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Guinea pig | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit | Dilution info - | Notes - |
Associated Products
Select an associated product type
10 products for Alternative Product
4 products for Alternative Version
Target data
Function
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Alternative names
Choline O-acetyltransferase, CHOACTase, ChAT, Choline acetylase, Chat
Recommended products
Rabbit Recombinant Monoclonal Choline Acetyltransferase antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Guinea pig, Rabbit samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR16590
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab221793 is the carrier-free version of Anti-Choline Acetyltransferase antibody [EPR16590] ab178850.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Choline Acetyltransferase (ChAT) also known as EC 2.3.1.6 acts as the enzyme responsible for synthesizing the neurotransmitter acetylcholine. It catalyzes the transfer of an acetyl group from acetyl-CoA to choline to form acetylcholine. ChAT is a protein with an approximate mass of 70 kDa and is expressed in cholinergic neurons throughout the central and peripheral nervous systems. High expression is observed in regions such as the basal forebrain and neuromuscular junctions which are important for cholinergic signaling.
- Biological function summary
The enzyme plays an important role in cholinergic conduction by producing acetylcholine which functions in synaptic transmission. It does not function as part of a larger complex but its activity directly influences cholinergic synapses. Choline acetyltransferase function ensures the synthesis and availability of acetylcholine for release into the synaptic cleft initiating muscle contraction and modulating activity in the central nervous system.
- Pathways
Choline acetyltransferase is critical in pathways governing neurotransmitter release such as the cholinergic synapse pathway. It interacts functionally with other proteins within this pathway including acetylcholinesterase which breaks down acetylcholine in the synaptic cleft. This interaction ensures proper neurotransmitter regulation and recycling maintaining synapse function and communication within neuronal circuits.
- Associated diseases and disorders
Alterations in choline acetyltransferase activity relate to neurological disorders including Alzheimer's disease and myasthenia gravis. In Alzheimer's disease reduced choline acetyltransferase function leads to decreased acetylcholine synthesis contributing to the cognitive decline observed in patients. In myasthenia gravis acetylcholine receptors are impaired but effective choline acetyltransferase activity is still necessary to manage neurotransmitter levels. A potential therapeutic angle involves targeting related proteins that exacerbate these conditions to support acetylcholine levels.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] - BSA and Azide free (ab221793), expandable thumbnail](https://content.abcam.com/products/images/choline-acetyltransferase-antibody-epr16590-bsa-and-azide-free-ab221793--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img70184.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] - BSA and Azide free (ab221793)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] - BSA and Azide free (ab221793)
Immunohistochemical analysis of paraffin-embedded Guinea Pig cerebral cortex tissue labeling Choline Acetyltransferase with Anti-Choline Acetyltransferase antibody [EPR16590] ab178850 at 1/2000 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasm staining on neurons of Guinea Pig cerebral cortex is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Choline Acetyltransferase antibody [EPR16590] ab178850).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] - BSA and Azide free (ab221793), expandable thumbnail](https://content.abcam.com/products/images/choline-acetyltransferase-antibody-epr16590-bsa-and-azide-free-ab221793--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img38102.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] - BSA and Azide free (ab221793)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] - BSA and Azide free (ab221793)
Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Choline Acetyltransferase with Anti-Choline Acetyltransferase antibody [EPR16590] ab178850 at 1/2000 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasm staining on neurons of Mouse cerebral cortex is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Choline Acetyltransferase antibody [EPR16590] ab178850).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] - BSA and Azide free (ab221793), expandable thumbnail](https://content.abcam.com/products/images/choline-acetyltransferase-antibody-epr16590-bsa-and-azide-free-ab221793--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134088.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] - BSA and Azide free (ab221793)")
This image is courtesy of an anonymous customer review.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline Acetyltransferase antibody [EPR16590] - BSA and Azide free (ab221793)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue sections labeling Choline Acetyltransferase with Anti-Choline Acetyltransferase antibody [EPR16590] ab178850 at 1/2000 dilution. Cells were fixed in 10% normal buffered formalin, heat mediated antigen retrieval was performed using TE pH9. A donkey anti-rabbit Alexa Fluor 647 was used as the secondary antibody at 1/300 dilution. Green was a false colour used to represent the Alexa Fluor 647 fluorescent signal.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Choline Acetyltransferase antibody [EPR16590] ab178850).
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