Rabbit Polyclonal Choline kinase alpha antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CHKA aa 50-200.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CHKA aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link P35790
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Plays a key role in phospholipid biosynthesis by catalyzing the phosphorylation of free choline to phosphocholine, the first step in phosphatidylcholine biosynthesis (PubMed:17007874, PubMed:19915674, PubMed:23416529, PubMed:34077757). Also phosphorylates ethanolamine, thereby contributing to phosphatidylethanolamine biosynthesis (PubMed:17007874, PubMed:19915674). Has higher activity with choline (PubMed:17007874, PubMed:19915674). May contribute to tumor cell growth (PubMed:19915674). Isoform 1. This isoform plays a key role in lipolysis of lipid droplets following glucose deprivation (PubMed:34077757). In response to glucose deprivation, phosphorylated by AMPK, promoting localization to lipid droplets (PubMed:34077757). Phosphorylation is followed by acetylation by KAT5, leading to dissociation of the homodimer into a monomer (PubMed:34077757). Monomeric CHKA isoform 1 is converted into a tyrosine-protein kinase, which phosphorylates lipid droplet structural proteins PLIN2 and PLIN3, leading to lipolysis of lipid droplets (PubMed:34077757).
Alternative names
CHK, CKI, CHKA, Choline kinase alpha, CK, CHETK-alpha, Ethanolamine kinase, EK
Recommended products
Rabbit Polyclonal Choline kinase alpha antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CHKA aa 50-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CHKA aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link P35790
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Choline kinase alpha also known as CKα or CHKA is an enzyme responsible for catalyzing the first step in the synthesis of phosphatidylcholine converting choline into phosphocholine. This process is important for membrane biosynthesis and cell signaling. Choline kinase alpha has a molecular mass of approximately 45 kDa. It is expressed in various tissues with high levels in liver brain and muscle reflecting its role in both metabolic and proliferative processes. The functional activity of CKα is influenced by its interaction with lipid environments and other cellular factors.
- Biological function summary
Choline kinase alpha plays a significant role in cell proliferation and survival due to its involvement in phospholipid metabolism. The enzyme is not known to be part of any large protein complexes but it does interact with a variety of cellular components that regulate its activity. It affects cellular processes by controlling the rate of phosphatidylcholine synthesis therefore impacting membrane dynamics and signal transduction. Changes in CKα activity can result in altered cell growth and function.
- Pathways
Choline kinase alpha is an essential part of the Kennedy pathway or the CDP-choline pathway which is a major pathway for phosphatidylcholine biosynthesis. Within this pathway CKα's activity is essential for progressing from choline uptake to the formation of phosphatidylcholine. Other key proteins related through this pathway include CTP:phosphocholine cytidylyltransferase (CCT) and cholinephosphotransferase. The enzyme's function integrates metabolic and signaling pathways connecting lipid metabolism with cellular responses to external stimuli.
- Associated diseases and disorders
Choline kinase alpha aberrations have been linked to cancer and neurodegenerative conditions. Overexpression of CKα is frequently observed in several types of cancer highlighting its role in tumor growth and metastasis. In cancer CKα interacts with oncogenic proteins that further drive malignancy. In neurodegenerative diseases altered choline metabolism may induce neuronal damage but the exact role of CKα in such conditions needs further investigation. Targeting CKα or its pathways could provide therapeutic potential for these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Choline kinase alpha antibody (ab235938)
All lanes: Western blot - Anti-Choline kinase alpha antibody (ab235938) at 1/500 dilution
All lanes: MCF7 (human breast adenocarcinoma cell line) whole cell lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Choline kinase alpha antibody (ab235938)
Paraffin-embedded human lung cancer tissue stained for Choline kinase alpha using ab235938 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-Choline kinase alpha antibody (ab235938)
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Choline kinase alpha (green) using ab235938 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L).
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