Anti-Choline kinase alpha antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Choline kinase alpha antibody (AB88053)

ICC/IF image of ab88053 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88053, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• ICC/IF

AbReview56968****

Immunocytochemistry/ Immunofluorescence - Anti-Choline kinase alpha antibody (AB88053)

Immunocytochemistry/ Immunofluorescence analysis of human glioblastoma cells labeling Choline kinase alpha with ab88053 at 1/250 dilution. Cells were fixed in paraformaldehyde and permeabilized with TritonX-100. A goat anti-rabbit Alexa Fluor594 was used as the secondary antibody at 1/1000 dilution.

This image is courtesy of an anonymous abreview.

• WB

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Western blot - Anti-Choline kinase alpha antibody (AB88053)

All lanes:

Western blot - Anti-Choline kinase alpha antibody (ab88053) at 1 µg/mL

Lane 1:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Human breast tissue lysate - total protein (<a href='/en-us/products/unavailable/human-breast-tissue-lysate-total-protein-ab30090'>ab30090</a>) at 10 µg

Lane 3:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 5:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 6:

A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa,58 kDa

true

Exposure time: 2min

• WB

Unknown

Western blot - Anti-Choline kinase alpha antibody (AB88053)

All lanes:

Western blot - Anti-Choline kinase alpha antibody (ab88053) at 1 µg/mL

Lane 1:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 2:

Western blot - Mouse lung normal tissue lysate - total protein (<a href='/en-us/products/tissue-lysates/mouse-lung-normal-tissue-lysate-total-protein-ab29297'>ab29297</a>) at 10 µg

Lane 3:

Brain (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa,72 kDa,78 kDa

true

Exposure time: 1min

• WB

AbReview56969****

Western blot - Anti-Choline kinase alpha antibody (AB88053)

All lanes:

Western blot - Anti-Choline kinase alpha antibody (ab88053) at 1/1000 dilution

Lane 1:

Choline kinase alpha knockdown Human Glioblastoma whole cell lysate at 50 µg

Lane 2:

Human Glioblastoma (Control) whole cell lysate at 50 µg

Secondary

All lanes:

goat-anti-rabbit IRDye800CW at 1/10000 dilution

Predicted band size: 52 kDa

false

Exposure time: 1min

This image is courtesy of an anonymous Abreview