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AB264484

Anti-Chondroitinase ABC I antibody [HL2010] - BSA and Azide free

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(1 Publication)

Mouse Recombinant Monoclonal Chondroitinase ABC I antibody. Carrier free. Suitable for WB, I-ELISA and reacts with Proteus vulgaris samples. Cited in 1 publication.

View Alternative Names

Chondroitin sulfate ABC endolyase, Chondroitin ABC endoeliminase, Chondroitin ABC lyase I, Chondroitin sulfate ABC lyase I, Chondroitinase ABC I, Endochondroitinase ABC, ChS ABC lyase I, cABC I

2 Images
Indirect ELISA - Anti-Chondroitinase ABC I antibody [HL2010] - BSA and Azide free (AB264484)
  • I-ELISA

Lab

Indirect ELISA - Anti-Chondroitinase ABC I antibody [HL2010] - BSA and Azide free (AB264484)

ELISA - Anti-Chondroitinase ABC I antibody [HL2010] (ab256485).

Antigen : Chondroitinase ABC at 1000 ng/ml.

ab256485 used at 20-5000 ng/mL.

Secondary is an Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) used at 1/1000 dilution.

This image was produced using the same antibody clone but in a different formulation ab256485, PBS, sodium azide, glycerol and BSA.

Western blot - Anti-Chondroitinase ABC I antibody [HL2010] - BSA and Azide free (AB264484)
  • WB

Lab

Western blot - Anti-Chondroitinase ABC I antibody [HL2010] - BSA and Azide free (AB264484)

Blocking/Dilution buffer : 5% NFDM/TBST.

This image was produced using the same antibody clone but in a different formulation ab256485, PBS, sodium azide, glycerol and BSA.

All lanes:

Western blot - Anti-Chondroitinase ABC I antibody [HL2010] (<a href='/en-us/products/primary-antibodies/chondroitinase-abc-i-antibody-hl2010-ab256485'>ab256485</a>) at 0.092 µg/mL

All lanes:

Proteus vulgaris Chondroitinase ABC recombinant Protein (aa25-1021), 20 ng

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

false

Exposure time: 3s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HL2010

Isotype

IgG1

Carrier free

Yes

Reacts with

Proteus vulgaris

Applications

WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Proteus vulgaris": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.092 µg/mL", "WB-species-notes": "<p></p>", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" } } }
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Product details

ab264484 is the carrier-free version of ab256485.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Chondroitinase ABC I also known as ChABC or Chondroitinase breaks down chondroitin sulfate and dermatan sulfate chains which are components of proteoglycans in the extracellular matrix. This enzyme with an approximate mass of 120 kDa originates from the bacterium Proteus vulgaris. Researchers often focus on its enzymatic function due to its ability to digest glycosaminoglycans thereby reducing inhibitory structures in the nervous system. Chondroitinase ABC I is not endogenously expressed in human tissue but its application involves tissue cultures and animal models.
Biological function summary

This enzyme plays a significant role in modulating the extracellular matrix by cleaving glycosaminoglycans removing barriers that can hinder cell regeneration. This activity indicates its potential for enhancing neural plasticity and aiding tissue repair particularly within the central nervous system. Although not requiring a complex for its activity Chondroitinase ABC I operates effectively in environments where its substrate is present facilitating processes important for remodeling the extracellular matrix.

Pathways

Chondroitinase ABC I contributes to the neural regeneration and repair pathway by altering the extracellular environment. Its function complements the actions of matrix metalloproteinases which also modify the extracellular matrix. The modulation of inhibitory proteoglycans aligns Chondroitinase ABC I with neuroplasticity pathways influencing neurite outgrowth and synaptic remodeling. This activity creates potential for pathways aiming at recovery from neural injuries and degeneration.

The use of Chondroitinase ABC I demonstrates potential therapeutic applications for conditions like spinal cord injury and multiple sclerosis. By facilitating neural repair and overcoming chondroitin sulfate proteoglycan-induced inhibition it offers promise in these challenging conditions. In the context of spinal cord injury Chondroitinase ABC I interacts functionally with proteins related to axonal regeneration such as Nogo receptor supporting its regenerative capability. In multiple sclerosis the enzyme's ability to alter the extracellular matrix may relieve some of the inhibitory effects faced during remyelination efforts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Endolytic, broad-specificity glycosaminoglycan lyase, which degrades the polysaccharides chondroitin, chondroitin-4-sulfate, chondroitin-6-sulfate, dermatan sulfate and to a lesser extent hyaluronan, by beta-elimination of 1,4-hexosaminidic bond to unsaturated tetrasaccharides and disaccharides. Is not active against keratan sulfate, heparan sulfate, and heparin. Is able to promote functional recovery in the injured central nervous system (CNS), via its role in the disruption of the normal organization of the extracellular matrix (ECM).
See full target information Chondroitin sulfate ABC endolyase

Additional targets

Chondroitinase ABC I
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 11:e0167682 PubMed27973564

2016

Chondroitinase C Selectively Degrades Chondroitin Sulfate Glycosaminoglycans that Inhibit Axonal Growth within the Endoneurium of Peripheral Nerve.

Applications

Unspecified application

Species

Unspecified reactive species

James B Graham,David Muir
View all publications
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Product promise

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