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AB194533

Anti-CHOP (phospho S30) antibody

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(4 Publications)

Rabbit Polyclonal CHOP phospho S30 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CHOP / DDIT3 phospho S30 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CHOP, CHOP10, GADD153, DDIT3, DNA damage-inducible transcript 3 protein, DDIT-3, C/EBP zeta, C/EBP-homologous protein, C/EBP-homologous protein 10, CCAAT/enhancer-binding protein homologous protein, Growth arrest and DNA damage-inducible protein GADD153, CHOP-10

1 Images
Western blot - Anti-CHOP (phospho S30) antibody (AB194533)
  • WB

Supplier Data

Western blot - Anti-CHOP (phospho S30) antibody (AB194533)

All lanes:

Western blot - Anti-CHOP (phospho S30) antibody (ab194533) at 1/500 dilution

Lane 1:

Jurkat cell extract, treated with PMA

Lane 2:

Jurkat cell extract, treated with PMA with antigen-specific peptide

Predicted band size: 19 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human DDIT3 phospho S30 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P35638

Specificity

ab194533 detects endogenous level of CHOP only when phosphorylated at serine 30.

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Reactivity data

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Product details

Previously called Anti-DDIT3 (phospho S30) antibody. The name of this product has been changed, there have been no other changes to the product.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab194533 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DDIT3 also known as CHOP (C/EBP homologous protein) is a transcription factor commonly expressed in cells under stress conditions such as endoplasmic reticulum stress. It has a molecular weight of approximately 29 kDa. As part of the system that manages cell stress responses DDIT3 expression can occur in various tissues but it is particularly notable in the context of cellular stress regulation. Researchers often use DDIT3 immunohistochemistry to study its presence and expression pattern in different tissues.
Biological function summary

DDIT3 plays a significant role in mediating cellular stress responses and promoting apoptosis when adaptive pathways fail. DDIT3 is not typically part of a multi-subunit complex but it acts in concert with other stress-related proteins to modulate gene expression. By inducing apoptosis DDIT3 helps remove severely damaged cells maintaining overall tissue health. However excessive DDIT3 activity under prolonged stress can lead to cell loss and tissue damage.

Pathways

DDIT3 is involved in the unfolded protein response (UPR) and endoplasmic reticulum stress pathways. It interacts with proteins such as ATF4 and PERK which are part of the mechanism that governs these pathways. DDIT3 induction contributes to the UPR pathway balancing the cell's fate between repair and apoptosis. This balance is important for cell survival under stress and avoids detrimental cellular damage.

DDIT3 has a strong connection with diabetes and neurodegenerative diseases. In diabetes DDIT3 overactivation can result in pancreatic beta-cell apoptosis worsening the disease's progression. Furthermore its involvement in neurodegenerative diseases includes its association with the accumulation of misfolded proteins exacerbating cellular stress conditions. DDIT3's interaction with proteins such as BCL2 further influences disease pathways by enhancing apoptotic signals.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multifunctional transcription factor in endoplasmic reticulum (ER) stress response (PubMed : 15322075, PubMed : 15775988, PubMed : 19672300). Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress (PubMed : 15322075, PubMed : 15775988). Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes (By similarity). Acts as a dominant-negative regulator of C/EBP-induced transcription : dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes (By similarity). Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L (PubMed : 15775988, PubMed : 17709599, PubMed : 20876114, PubMed : 22761832). Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG) (PubMed : 18940792, PubMed : 19672300, PubMed : 20829347). Together with ATF4, mediates ER-mediated cell death by promoting expression of genes involved in cellular amino acid metabolic processes, mRNA translation and the unfolded protein response (UPR) in response to ER stress (By similarity). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity (PubMed : 16434966). Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response (By similarity). Acts as a major regulator of postnatal neovascularization through regulation of endothelial nitric oxide synthase (NOS3)-related signaling (By similarity).
See full target information DDIT3 phospho S30
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:13667-13679 PubMed35703318

2022

lncRNA NBR2 attenuates angiotensin II-induced myocardial hypertrophy through repressing ER stress via activating LKB1/AMPK/Sirt1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Cansheng Zhu,Min Wang,Xianguan Yu,Xing Shui,Leile Tang,Zefeng Chen,Zhaojun Xiong

Bosnian journal of basic medical sciences 22:191-204 PubMed34582743

2022

Quercetin ameliorates testosterone secretion disorder by inhibiting endoplasmic reticulum stress through the miR-1306-5p/HSD17B7 axis in diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Di Wang,Yan Li,Qian-Qian Zhai,Yun-Feng Zhu,Bei-Yan Liu,Yun Xu

Molecular medicine reports 25: PubMed35348185

2022

CTRP9 decreases high glucose‑induced trophoblast cell damage by reducing endoplasmic reticulum stress.

Applications

Unspecified application

Species

Unspecified reactive species

Lianxiao Zhang,Huiqing Ding,Yubo Shi,Duoyi Zhang,Xue Yang

Veterinary microbiology 255:109013 PubMed33676093

2021

Mycoplasmas bovis P48 induces apoptosis in EBL cells via an endoplasmic reticulum stress-dependent signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaochun Wu,Shengying Zhang,Cuiqin Long,Zhen An,Xiaoyong Xing,Fengqin Wen,Shijun Bao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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