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AB279579

Anti-Chordin antibody

0

(1 Review)

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(1 Publication)

Rabbit Polyclonal Chordin antibody. Suitable for ICC, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CHRD.

View Alternative Names

UNQ217/PRO243, CHRD, Chordin

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chordin antibody (AB279579)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chordin antibody (AB279579)

Immunohistochemical analysis of formalin fixed, paraffin-embedded human hepatoma labelling human Chordin using ab279579 at 1 : 1000 dilution.

Immunocytochemistry - Anti-Chordin antibody (AB279579)
  • ICC

Supplier Data

Immunocytochemistry - Anti-Chordin antibody (AB279579)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized and 10% serum blocked U2OS cells stained for human Chordin using ab279579 at 1/1000 dilution at 4℃ overnight, followed by Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and nuclear counter stain (blue). Positive staining was localized to Nucleus.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC

applications

Immunogen

Synthetic Peptide within Human CHRD. The exact immunogen used to generate this antibody is proprietary information.

Q9H2X0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/300 - 1/10000", "ICC-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification notes
Protein A & Antigen Affinity chromatography.
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Chordin also known as CHRD is a protein important in vertebrate embryonic development. It weighs approximately 120 kDa. Chordin mainly operates by binding and inhibiting the activity of bone morphogenetic proteins (BMPs) which are part of the TGF-beta superfamily. It gets expressed in the dorsal mesoderm during early development and in various tissues in adult organisms including the central nervous system and skeletal tissue.
Biological function summary

Chordin functions by modulating the effects of BMP signaling which is important for proper tissue differentiation. Chordin acts as a BMP antagonist by binding to BMPs preventing their interaction with receptors. This interaction allows the establishment of a morphogen gradient essential for dorsal-ventral axis formation during embryonic stages. Chordin is not known to form part of a larger molecular complex; rather it functions as a single agent in the inhibition of BMP interactions.

Pathways

Chordin plays a role in the BMP signaling and the dorsoventral axis formation pathways. It interacts with BMP proteins like BMP-4 to balance signals needed for correct tissue development. The secretion of Chordin and subsequent BMP binding affect signaling through pathways such as the Smad proteins which mediate BMP signal transduction. This results in spatial and temporal regulation of gene expression necessary for developmental processes.

Disruptions in Chordin function are associated with congenital malformations like the Arnold-Chiari malformation and congenital heart disease. Incorrect BMP signaling due to altered Chordin activity has been shown to contribute to these conditions. Additionally defects in BMP pathway regulation involving Chordin can influence interactions with proteins such as Noggin which further manage BMP activity and can compound disease outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes (By similarity).
See full target information CHRD
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2401478 PubMed38785178

2024

Formulate Adaptive Biphasic Scaffold via Sequential Protein-Instructed Peptide Co-Assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Yazhou Chen,Qizheng Zhang,Shenyu Yang,Guanying Li,Chaochen Shi,Xunwu Hu,Shunsuke Asahina,Natsuko Asano,Ye Zhang
View all publications
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