Anti-CHRDL1 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CHRDL1 antibody (AB227473)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling CHRDL1 protein at cytoplasm using ab227473 at a 1/500 dilution (Green).
alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/1000 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue). Scale bar = 10 µm

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRDL1 antibody (AB227473)

Paraffin-embedded mouse hind brain tissue stained for CHRDL1 with ab227473 at 1/500 dilution in immunohistochemical analysis.

• WB

Supplier Data

Western blot - Anti-CHRDL1 antibody (AB227473)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-CHRDL1 antibody (ab227473) at 1/5000 dilution

Lane 1:

Untreated HeLa cell lysates at 30 µg

Lane 2:

HeLa cell lysates treated with 1% O₂, 4hr at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 52 kDa

false

• WB

Supplier Data

Western blot - Anti-CHRDL1 antibody (AB227473)

10% SDS-PAGE

All lanes:

Western blot - Anti-CHRDL1 antibody (ab227473) at 1/500 dilution

All lanes:

Rat brain tissue extract at 50 µg

Predicted band size: 51 kDa

true

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRDL1 antibody (AB227473)

Paraffin-embedded mouse kidney tissue stained for CHRDL1 with ab227473 at 1/500 dilution in immunohistochemical analysis.