JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB325865

Anti-CHRM1 antibody [HL3933] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Muscarinic Acetylcholine Receptor 1/CHRM1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human CHRM1.

View Alternative Names

Muscarinic acetylcholine receptor M1, CHRM1

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRM1 antibody [HL3933] - BSA and Azide free (AB325865)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRM1 antibody [HL3933] - BSA and Azide free (AB325865)

ab325865 detects mAChR M1 protein by immunohistochemical analysis. Sample : Paraffin-embedded human tissues. mAChR M1 stained by ab325865 diluted at 1 : 200. Antigen Retrieval : Tris-EDTA buffer, pH 9.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRM1 antibody [HL3933] - BSA and Azide free (AB325865)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRM1 antibody [HL3933] - BSA and Azide free (AB325865)

ab325865 detects mAChR M1 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse striatum. Green : mAChR M1 stained by ab325865 diluted at 1 : 250. Red : beta Tubulin 3/ Tuj1 antibody diluted at 1 : 500. Blue : Hoechst 33342 staining. Antigen Retrieval : Tris-EDTA buffer, pH 9.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRM1 antibody [HL3933] - BSA and Azide free (AB325865)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRM1 antibody [HL3933] - BSA and Azide free (AB325865)

ab325865 detects mAChR M1 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse brain. mAChR M1 stained by ab325865 diluted at 1 : 2000. Antigen Retrieval : Tris-EDTA buffer, pH 9.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRM1 antibody [HL3933] - BSA and Azide free (AB325865)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRM1 antibody [HL3933] - BSA and Azide free (AB325865)

ab325865 detects mAChR M1 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse tissues. mAChR M1 stained by ab325865 diluted at 1 : 2000. Antigen Retrieval : Tris-EDTA buffer, pH 9.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRM1 antibody [HL3933] - BSA and Azide free (AB325865)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRM1 antibody [HL3933] - BSA and Azide free (AB325865)

ab325865 detects mAChR M1 protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse cerebral cortex. Green : mAChR M1 stained by ab325865 diluted at 1 : 250. Red : beta Tubulin 3/ Tuj1 antibody diluted at 1 : 500. Blue : Hoechst 33342 staining. Antigen Retrieval : Tris-EDTA buffer, pH 9.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRM1 antibody [HL3933] - BSA and Azide free (AB325865)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRM1 antibody [HL3933] - BSA and Azide free (AB325865)

ab325865 detects mAChR M1 protein by immunohistochemical analysis. Sample : Paraffin-embedded rat tissues. mAChR M1 stained by ab325865 diluted at 1 : 200. Antigen Retrieval : Tris-EDTA buffer, pH 9.0, 15 min.

Western blot - Anti-CHRM1 antibody [HL3933] - BSA and Azide free (AB325865)
  • WB

Supplier Data

Western blot - Anti-CHRM1 antibody [HL3933] - BSA and Azide free (AB325865)

Unboiled various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325865 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CHRM1 antibody [HL3933] - BSA and Azide free (ab325865) at 1/1000 dilution

Lane 1:

Unboiled various mouse brain tissue extracts at 50 µg

Lane 2:

Unboiled various mouse muscle tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-CHRM1 antibody [HL3933] - BSA and Azide free (AB325865)
  • WB

Supplier Data

Western blot - Anti-CHRM1 antibody [HL3933] - BSA and Azide free (AB325865)

Unboiled rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ab325865 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CHRM1 antibody [HL3933] - BSA and Azide free (ab325865) at 1/1000 dilution

All lanes:

Unboiled rat brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL3933

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human CHRM1.

P11229

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
See full target information CHRM1
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com