AB129276

Anti-CHRNB4 antibody

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(5 Publications)

Goat Polyclonal CHRNB4 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human CHRNB4 aa 50-150.

View Alternative Names

Neuronal acetylcholine receptor subunit beta-4, CHRNB4

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRNB4 antibody (AB129276)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRNB4 antibody (AB129276)

ab129276, at 3.75 μg/ml, staining CHRNB4 in Formalin-fixed, Paraffin-embedded Human uterus tissue by Immunohistochemistry.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human CHRNB4 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

P30926

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5-3.75 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Common marmoset": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Elephant": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators (PubMed : 20881005, PubMed : 31488329, PubMed : 8663494, PubMed : 8906617, PubMed : 9203638). CHRNB4 forms heteropentameric neuronal acetylcholine receptors with CHRNA2, CHRNA3 and CHRNA4, as well as CHRNA5 and CHRNB3 as accesory subunits (PubMed : 11118490, PubMed : 20881005, PubMed : 8663494). CHRNA3 : CHRNB4 being predominant in neurons of the autonomic ganglia, it is known as ganglionic nicotinic receptor (PubMed : 31488329). CHRNA3 : CHRNB4 or CHRNA3 : CHRNA5 : CHRNB4 play also an important role in the habenulo-interpeduncular tract, modulating the mesolimbic dopamine system and affecting reward circuits and addiction (By similarity). Hypothalamic CHRNA3 : CHRNB4 nAChR activation by nicotine leads to activation of POMC neurons and a decrease in food intake (By similarity).

See full target information CHRNB4

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 10:4037 PubMed31492869

2019

Central nicotine induces browning through hypothalamic κ opioid receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia Seoane-Collazo,Laura Liñares-Pose,Eva Rial-Pensado,Amparo Romero-Picó,José María Moreno-Navarrete,Noelia Martínez-Sánchez,Pablo Garrido-Gil,Ramón Iglesias-Rey,Donald A Morgan,Naoki Tomasini,Samuel Andrew Malone,Ana Senra,Cintia Folgueira,Gema Medina-Gomez,Tomás Sobrino,José L Labandeira-García,Rubén Nogueiras,Ana I Domingos,José-Manuel Fernández-Real,Kamal Rahmouni,Carlos Diéguez,Miguel López

Scientific reports 9:3805 PubMed30846819

2019

Direct Effects of Nicotine Exposure on Murine Calvaria and Calvarial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Emily Durham,R Nicole Howie,Graham Warren,Amanda LaRue,James Cray

PloS one 13:e0194089 PubMed29513745

2018

Nicotinic acetylcholine receptor (CHRN) expression and function in cultured human adult fungiform (HBO) taste cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Qian,Shobha Mummalaneni,James Larsen,John R Grider,Andrew I Spielman,Mehmet Hakan Özdener,Vijay Lyall

PloS one 13:e0190465 PubMed29293602

2018

Nicotinic acetylcholine receptors (nAChRs) are expressed in Trpm5 positive taste receptor cells (TRCs).

Applications

Unspecified application

Species

Unspecified reactive species

Jie Qian,Shobha Mummalaneni,John R Grider,M Imad Damaj,Vijay Lyall

PloS one 11:e0166565 PubMed27846263

2016

Nicotine-Induced Effects on Nicotinic Acetylcholine Receptors (nAChRs), Ca2+ and Brain-Derived Neurotrophic Factor (BDNF) in STC-1 Cells.

Applications

ICC/IF

Species

Mouse

Jie Qian,Shobha K Mummalaneni,Reem M Alkahtani,Sunila Mahavadi,Karnam S Murthy,John R Grider,Vijay Lyall

View all publications

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Product promise

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Anti-CHRNB4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRNB4 antibody (AB129276)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Product promise