AB114115

Anti-Chromodomain helicase DNA binding protein 9 antibody

Name: Anti-Chromodomain helicase DNA binding protein 9 antibody (ab114115) | Abcam
Brand: Abcam Limited
SKU: AB114115
Price: 535 USD
Availability: InStock

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Polyclonal Chromodomain helicase DNA binding protein 9 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CHD9 aa 1-100.

View Alternative Names

KIAA0308, KISH2, PRIC320, AD-013, x0008, CHD9, Chromodomain-helicase-DNA-binding protein 9, CHD-9, ATP-dependent helicase CHD9, Chromatin-related mesenchymal modulator, Chromatin-remodeling factor CHROM1, Kismet homolog 2, PPAR-alpha-interacting complex protein 320 kDa, Peroxisomal proliferator-activated receptor A-interacting complex 320 kDa protein, CReMM

2 Images

Unknown

Immunoprecipitation - Anti-Chromodomain helicase DNA binding protein 9 antibody (AB114115)

Detection of Chromodomain helicase DNA binding protein 9 by Western Blot of Immunprecipitate.
Lane 1 : ab114115 at 1µg/ml staining Chromodomain helicase DNA binding protein 9 in HeLa whole cell lysate immunoprecipitated using ab114115 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2 : Control IgG
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-Chromodomain helicase DNA binding protein 9 antibody (ab114115)

Predicted band size: 326 kDa

false

Unknown

Western blot - Anti-Chromodomain helicase DNA binding protein 9 antibody (AB114115)

All lanes:

Western blot - Anti-Chromodomain helicase DNA binding protein 9 antibody (ab114115) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 326 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human CHD9 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q3L8U1

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab114115 was affinity purified using an epitope specific to Chromodomain helicase DNA binding protein 9 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Chromodomain helicase DNA binding protein 9 (CHD9) also known as CHD Family Member 9 possesses the function of chromatin remodeling. This protein belongs to the CHD family characterized by the presence of chromodomains that facilitate its role in modifying chromatin structure. CHD9 has a molecular weight of approximately 380 kDa. The expression of CHD9 is varied with higher levels observed in tissues such as the brain liver and heart indicating its broader involvement in cellular processes across different tissues.

Biological function summary

CHD9 plays a role in transcription regulation by altering chromatin configuration to allow access to transcription factors. It associates with other protein complexes to exert its influence on gene expression. Its ability to bind DNA and modify chromatin structure enables the modulation of genes necessary for cellular differentiation and growth processes. The protein's involvement as part of chromatin-remodeling complexes highlights its importance in maintaining the dynamic nature of chromatin structure.

Pathways

CHD9 influences gene expression through interaction with the Wnt and MAPK signaling pathways. It participates in these signaling pathways alongside key players such as β-catenin and ERK proteins which regulate cell proliferation and differentiation. The participation of CHD9 in these pathways highlights its role in cellular signaling and development connecting it to critical cellular functions.

CHD9 shows relevance in certain cancers and neurodevelopmental disorders. Its role in chromatin remodeling ties it to abnormalities in transcription that can contribute to oncogenesis commonly linked with overexpression of other chromatin remodelers. Additionally connections to neurodevelopmental disorders emerge from its expression profile and interaction with proteins such as MeCP2 which are vital in neural development and function.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Acts as a transcriptional coactivator for PPARA and possibly other nuclear receptors. Proposed to be a ATP-dependent chromatin remodeling protein. Has DNA-dependent ATPase activity and binds to A/T-rich DNA. Associates with A/T-rich regulatory regions in promoters of genes that participate in the differentiation of progenitors during osteogenesis (By similarity).

See full target information CHD9

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell cycle (Georgetown, Tex.) 21:585-601 PubMed35100082

2022

MiR-130b-3p promotes colorectal cancer progression by targeting CHD9.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Song,Qian Zhang,Hao Zhang,Liangliang Zhan,Xinchen Sun

Journal of vascular research 58:180-190 PubMed33794525

2021

MiR-22-3p Suppresses Vascular Remodeling and Oxidative Stress by Targeting CHD9 during the Development of Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Hanqing Chen,Xiru Xu,Zhengqing Liu,Yong Wu

Stem cells international 2017:1732094 PubMed29358953

2017

Allogeneic Umbilical Cord-Derived Mesenchymal Stem Cells as a Potential Source for Cartilage and Bone Regeneration: An Study.

Applications

Unspecified application

Species

Unspecified reactive species

A Marmotti,S Mattia,F Castoldi,A Barbero,L Mangiavini,D E Bonasia,M Bruzzone,F Dettoni,R Scurati,G M Peretti

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Chromodomain helicase DNA binding protein 9 antibody

Immunoprecipitation - Anti-Chromodomain helicase DNA binding protein 9 antibody (AB114115)

Western blot - Anti-Chromodomain helicase DNA binding protein 9 antibody (AB114115)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise