Anti-chromogranin A antibody is a rabbit polyclonal antibody that is used to detect chromogranin A in IHC. Suitable for human samples.
- Cited in >235 publications
- Tried and trusted by researchers since 2004
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- Nature communications 14:84842023Adult stem cell activity in naked mole rats for long-term tissue maintenance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShamir Montazid,Sheila Bandyopadhyay,Daniel W Hart,Nan Gao,Brian Johnson,Sri G Thrumurthy,Dustin J Penn,Bettina Wernisch,Mukesh Bansal,Philipp M Altrock,Fabian Rost,Patrycja Gazinska,Piotr Ziolkowski,Bu'Hussain Hayee,Yue Liu,Jiangmeng Han,Annamaria Tessitore,Jana Koth,Walter F Bodmer,James E East,Nigel C Bennett,Ian Tomlinson,Shazia IrshadPubMed 38123565
- Pediatric surgery international 40:62023Generation of human gastric assembloids from primary fetal organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiada Benedetti,Brendan C Jones,Francesca Sgualdino,Paolo De Coppi,Giovanni Giuseppe GiobbePubMed 37999863
- Science advances 9:eadi18272023Stromal BMP signaling regulates mucin production in the large intestine via interleukin-1/17.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYalong Wang,Ruoyu Lou,Zhe Zhang,Chuyu Xiao,Shicheng Yu,Siting Wei,Yuan Liu,Wei Fu,Baojie Li,Ye-Guang ChenPubMed 37889976
- International journal of molecular sciences 24:2023Novel Mouse Cell Lines and In Vivo Models for Human High-Grade Neuroendocrine Lung Carcinoma, Small Cell Lung Carcinoma (SCLC), and Large Cell Neuroendocrine Carcinoma (LCNEC).Applications:Unspecified applicationReactive species:Unspecified reactive speciesEnrique Recuero,Sara Lázaro,Corina Lorz,Ana Belén Enguita,Ramón Garcia-Escudero,Mirentxu SantosPubMed 37894963
- Animals : an open access journal from MDPI 13:2023Maternal Folic Acid Supplementation Improves the Intestinal Health of Offspring Porcine by Promoting the Proliferation and Differentiation of Intestinal Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuhui Zhang,Xiaofeng Zhang,Jianjun Chen,Shouchuan Jiang,Yu Han,Huahua DuPubMed 37835698
- Nature communications 14:61092023IFNγ-Stat1 axis drives aging-associated loss of intestinal tissue homeostasis and regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOmid Omrani,Anna Krepelova,Seyed Mohammad Mahdi Rasa,Dovydas Sirvinskas,Jing Lu,Francesco Annunziata,George Garside,Seerat Bajwa,Susanne Reinhardt,Lisa Adam,Sandra Käppel,Nadia Ducano,Daniela Donna,Alessandro Ori,Salvatore Oliviero,Karl Lenhard Rudolph,Francesco NeriPubMed 37777550
- Cell death & disease 14:6392023MRTF-A gain-of-function in mice impairs homeostatic renewal of the intestinal epithelium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnurag Kumar Singh,Amrita Rai,Anja Weber,Martin Gericke,Klaus-Peter Janssen,Markus Moser,Guido PosernPubMed 37770456
- Development (Cambridge, England) 150:2023Human prostate organoid generation and the identification of prostate development drivers using inductive rodent tissues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesParmveer Singh,Nadia A Lanman,Hannah L R Kendall,Laura Wilson,Ryan Long,Omar E Franco,Adriana Buskin,Colin G Miles,Simon W Hayward,Rakesh Heer,Craig N RobsonPubMed 37376888
- International journal of bioprinting 9:7232023Using 3D-bioprinted models to study pediatric neural crest-derived tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesColin H Quinn,Andee M Beierle,Janet R Julson,Michael E Erwin,Hasan Alrefai,Hooper R Markert,Jerry E Stewart,Sara Claire Hutchins,Laura V Bownes,Jamie M Aye,Elizabeth Mroczek-Musulman,Patricia H Hicks,Karina J Yoon,Christopher D Willey,Elizabeth A BeierlePubMed 37323483
- iScience 26:1065002023Loss of Fgf9 in mice leads to pancreatic hypoplasia and asplenia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSophie Patzek,Zhe Liu,Sean de la O,Sean Chang,Lauren E Byrnes,Xiuqin Zhang,David M Ornitz,Julie B SneddonPubMed 37096042
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Not recommended | Tested |
| Mouse | Not recommended | Predicted |
| Monkey | Not recommended | Predicted |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Monkey | Dilution info - | Notes - |
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Target data
Function
Pancreastatin. Strongly inhibits glucose induced insulin release from the pancreas. Catestatin. Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed:15326220). Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed:15723172, PubMed:24723458). Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed:21214543). Acts as a potent scavenger of free radicals in vitro (PubMed:24723458). May play a role in the regulation of cardiac function and blood pressure (PubMed:18541522). Serpinin. Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.
Alternative names
Chromogranin-A, CgA, Pituitary secretory protein I, SP-I, CHGA
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Anti-chromogranin A antibody is a rabbit polyclonal antibody that is used to detect chromogranin A in IHC. Suitable for human samples.
- Cited in >235 publications
- Tried and trusted by researchers since 2004
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Product Specifications
Anti-Chromogranin A antibody (ab15160) is a rabbit polyclonal antibody and is validated for use in IHC-P in human samples.
Anti-Chromogranin A antibody (ab15160) specifically detects Chromogranin A (UniProt ID: P10645; Molecular weight: 49kDa) and is sold in 100 µL, 500 µL and 1 mL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-Chromogranin A antibody (ab15160) has high sensitivity and specificity.
Anti-Chromogranin A antibody (ab15160) has been cited over 238 times in peer reviewed journals and is trusted by the scientific community.
Anti-Chromogranin A antibody (ab15160) has 29 independent reviews from customers.
Target Information
Chromogranin A (CgA) or parathyroid secretory protein 1 is encoded in the human by the gene CHGA. Chromogranin A (CgA) is found in the secretory vesicles of neuroendocrine cells and serves as a key biomarker in oncology. It is particularly useful in diagnosing and monitoring neuroendocrine tumors (NETs).
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Chromogranin A (CgA) sometimes called chromagranin A is a glycoprotein with a molecular weight of approximately 49–52 kDa. It is highly expressed in the secretory granules of neuroendocrine cells present in tissues such as the adrenal medulla pancreas and endocrine cells of the gastrointestinal tract. CgA identified as part of a family including chromogranin B and chromogranin C functions mechanically as a precursor to various bioactive peptides. This translates into its involvement in the storage and release of hormones and peptides within vesicles.
- Biological function summary
Chromogranin A assists in the regulation of secretory pathways across multiple neuroendocrine systems. It plays a role in hormone storage conversion to active peptides and regulates the exocytosis of hormones. CgA acts within a complex contributing to secretory granule biogenesis and influencing intravesicular acidity. As a regulatory component the exact physiological roles are diverse impacting cardiovascular metabolic and immunological processes.
- Pathways
Chromogranin A is involved in pathways such as the catecholamine synthesis and release pathway and the serotonergic pathway. Within these pathways it interacts with proteins like secretogranin II and parathyroid hormone (PTH). These interactions modulate neurotransmitter storage and secretion across various systems. CgA's bioactive peptides also contribute to modulating physiological processes like vasoconstriction and insulin regulation linking it closely with these signaling cascades.
- Associated diseases and disorders
Chromogranin A is a known marker for neuroendocrine tumors where its levels in blood may increase due to tumor activity. It links to diseases like pheochromocytoma and carcinoid tumors where improper regulation of CgA-associated pathways occurs. Additionally its connection to parathyroid disorders through the PTH pathway illustrates its broader implication in endocrine pathophysiology. CgA serves as an invaluable diagnostic and prognostic tool in these disease states and supports the development of targeted therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody (ab15160)
IHC image of Chromogranin A staining in a section of formalin-fixed paraffin-embedded normal human pancreas* performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab15160, 1/500 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Western blot - Anti-Chromogranin A antibody (ab15160)
Chromogranin A is easily fragmented and both bands observed in the Western Blot above are believed to correspond to Chromogranin A.
All lanes: Western blot - Anti-Chromogranin A antibody (ab15160) at 1/100 dilution
All lanes: Western blot - Recombinant Human Chromogranin A protein (Recombinant Human Chromogranin A protein ab85486) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Exposure time: 30s
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