AB17064

Anti-Chromogranin A antibody

Name: Anti-Chromogranin A antibody (ab17064) | Abcam
Brand: Abcam Limited
SKU: AB17064

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(6 Publications)

Rabbit Polyclonal Chromogranin A antibody. Suitable for IHC-P, sELISA and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human CHGA.

View Alternative Names

Chromogranin-A, CgA, Pituitary secretory protein I, SP-I, CHGA

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody (AB17064)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody (AB17064)

ab17064 staining Chromogranin A in formalin-fixed, paraffin-embedded Human pancreas tissue by Immunohistochemistry.

Sandwich ELISA - Anti-Chromogranin A antibody (AB17064)

sELISA

Unknown

Sandwich ELISA - Anti-Chromogranin A antibody (AB17064)

Standard Curve for Chromogranin A (Analyte : ab85486) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [23A1] to Chromogranin A (ab36997) at 1ug/ml and Detector Antibody Rabbit polyclonal to Chromogranin A (ab17064) at 0.5ug/ml. Concentration of ab17064 may vary from lot to lot; please use this curve as guideline.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, sELISA

applications

Immunogen

Native Full Length Protein corresponding to Human CHGA.

P10645

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>For sandwich ELISA, use this antibody as Detection with <a href='/en-us/products/unavailable/chromogranin-a-antibody-23a1-ab36997'>ab36997</a> as Capture.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Chromogranin A (CgA) sometimes called chromagranin A is a glycoprotein with a molecular weight of approximately 49–52 kDa. It is highly expressed in the secretory granules of neuroendocrine cells present in tissues such as the adrenal medulla pancreas and endocrine cells of the gastrointestinal tract. CgA identified as part of a family including chromogranin B and chromogranin C functions mechanically as a precursor to various bioactive peptides. This translates into its involvement in the storage and release of hormones and peptides within vesicles.

Biological function summary

Chromogranin A assists in the regulation of secretory pathways across multiple neuroendocrine systems. It plays a role in hormone storage conversion to active peptides and regulates the exocytosis of hormones. CgA acts within a complex contributing to secretory granule biogenesis and influencing intravesicular acidity. As a regulatory component the exact physiological roles are diverse impacting cardiovascular metabolic and immunological processes.

Pathways

Chromogranin A is involved in pathways such as the catecholamine synthesis and release pathway and the serotonergic pathway. Within these pathways it interacts with proteins like secretogranin II and parathyroid hormone (PTH). These interactions modulate neurotransmitter storage and secretion across various systems. CgA's bioactive peptides also contribute to modulating physiological processes like vasoconstriction and insulin regulation linking it closely with these signaling cascades.

Chromogranin A is a known marker for neuroendocrine tumors where its levels in blood may increase due to tumor activity. It links to diseases like pheochromocytoma and carcinoid tumors where improper regulation of CgA-associated pathways occurs. Additionally its connection to parathyroid disorders through the PTH pathway illustrates its broader implication in endocrine pathophysiology. CgA serves as an invaluable diagnostic and prognostic tool in these disease states and supports the development of targeted therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Pancreastatin. Strongly inhibits glucose induced insulin release from the pancreas.. Catestatin. Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed : 15326220). Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed : 15723172, PubMed : 24723458). Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed : 21214543). Acts as a potent scavenger of free radicals in vitro (PubMed : 24723458). May play a role in the regulation of cardiac function and blood pressure (PubMed : 18541522).. Serpinin. Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.

See full target information CHGA

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular biochemistry 478:2779-2787 PubMed36920577

2023

Individuals with type 2 diabetes have higher density of small intestinal neurotensin-expressing cells.

Applications

Unspecified application

Species

Unspecified reactive species

Filipa P Ferreira,Sofia S Pereira,Madalena M Costa,Marta Guimarães,Nicolai J Wewer Albrechtsen,Jens J Holst,Mário Nora,Mariana P Monteiro

Gastroenterology 163:411-425.e4 PubMed35487288

2022

Toll-like Receptor 9 Pathway Mediates Schlafen-MDSC Polarization During Helicobacter-induced Gastric Metaplasias.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Ding,Jayati Chakrabarti,Sulaiman Sheriff,Qian Li,Hahn Nguyen Thi Hong,Ricky A Sontz,Zoe E Mendoza,Amanda Schreibeis,Michael A Helmrath,Yana Zavros,Juanita L Merchant

Journal of cellular biochemistry 119:7506-7514 PubMed29761876

2018

Differential GIP/GLP-1 intestinal cell distribution in diabetics' yields distinctive rearrangements depending on Roux-en-Y biliopancreatic limb length.

Applications

Unspecified application

Species

Unspecified reactive species

António M Palha,Sofia S Pereira,Madalena M Costa,Tiago Morais,André F Maia,Marta Guimarães,Mário Nora,Mariana P Monteiro

Cellular and molecular gastroenterology and hepatology 2:131-157 PubMed27019864

2016

Loss of MYO5B in mice recapitulates Microvillus Inclusion Disease and reveals an apical trafficking pathway distinct to neonatal duodenum.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria G Weis,Byron C Knowles,Eunyoung Choi,Anna E Goldstein,Janice A Williams,Elizabeth H Manning,Joseph T Roland,Lynne A Lapierre,James R Goldenring

Cellular and molecular gastroenterology and hepatology 1:233-247.e1 PubMed28247862

2014

Growth Factor Independence-1 () Is Required for Pancreatic Acinar Unit Formation and Centroacinar Cell Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoling Qu,Pia Nyeng,Fan Xiao,Jorge Dorantes,Jan Jensen

FASEB journal : official publication of the Federa 26:3127-39 PubMed22516298

2012

A high-fat diet regulates gastrin and acid secretion through primary cilia.

Applications

IHC-P

Species

Mouse

Milena Saqui-Salces,William E Dowdle,Jeremy F Reiter,Juanita L Merchant

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Chromogranin A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody (AB17064)

Sandwich ELISA - Anti-Chromogranin A antibody (AB17064)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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