Rabbit Polyclonal Chromogranin A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 35 publications.
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- Gut microbes 15:22331492023Fucose promotes intestinal stem cell-mediated intestinal epithelial development through promoting -related propanoate metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCaihan Duan,Junhao Wu,Zhe Wang,Chen Tan,Lingzhi Hou,Wei Qian,Chaoqun Han,Xiaohua HouPubMed 37424378
- Animal nutrition (Zhongguo xu mu shou yi xue hui) 14:43-552023Porcine intestinal antimicrobial peptide as an in-feed antibiotic alternative improves intestinal digestion and immunity by shaping the gut microbiota in weaned piglets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFengjie Ji,Huansheng Yang,Qiye Wang,Jianzhong Li,Hanlin Zhou,Shengmin LiuPubMed 37234948
- The Journal of cell biology 222:2023Antagonism between Prdm16 and Smad4 specifies the trajectory and progression of pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEric Hurwitz,Parash Parajuli,Seval Ozkan,Celine Prunier,Thien Ly Nguyen,Deanna Campbell,Creighton Friend,Allyn Austin Bryan,Ting-Xuan Lu,Steven Christopher Smith,Mohammed Shawkat Razzaque,Keli Xu,Azeddine AtfiPubMed 36828547
- Frontiers in immunology 14:10835132023Dysfunction of the noradrenergic system drives inflammation, α-synucleinopathy, and neuronal loss in mouse colon.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSheng Song,Dezhen Tu,Chengbo Meng,Jie Liu,Belinda Wilson,Qingshan Wang,Yen-Yu Ian Shih,Hui-Ming Gao,Jau-Shyong HongPubMed 36845109
- Frontiers in pharmacology 13:10104842023Aqueous cinnamon extract ameliorates bowel dysfunction and enteric 5-HT synthesis in IBS rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLijuan Yu,Chunhua Huang,Wei Yang,Zhenxing Ren,Lifeng Li,Huiyuan Cheng,Chengyuan Lin,Lixiang Zhai,Ziwan Ning,Hoileong Xavier Wong,Quanbin Han,Wei Jia,Zhaoxiang Bian,Ling ZhaoPubMed 36699075
- Medicina (Kaunas, Lithuania) 59:2022Protective Effect of Irsogladine against Aspirin-Induced Mucosal Injury in Human Induced Pluripotent Stem Cell-Derived Small Intestine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakuya Kanno,Takahito Katano,Isamu Ogawa,Takahiro Iwao,Tamihide Matsunaga,Hiromi KataokaPubMed 36676718
- Open biology 12:2203222022Characterization of insulin crystalline form in isolated β-cell secretory granules.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeiya Asai,Jana Moravcová,Lenka Žáková,Irena Selicharová,Romana Hadravová,Andrzej Marek Brzozowski,Jiří Nováček,Jiří JiráčekPubMed 36541100
- Cell death & disease 13:9272022Androgen deprivation restores ARHGEF2 to promote neuroendocrine differentiation of prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuanrong Chen,Yi Shao,Wanqing Wei,Shimiao Zhu,Yang Li,Yutong Chen,Hanling Li,Hao Tian,Guijiang Sun,Yuanjie Niu,Zhiqun ShangPubMed 36335093
- JCI insight 7:2022Immune cells and their inflammatory mediators modify β cells and cause checkpoint inhibitor-induced diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna Luisa Perdigoto,Songyan Deng,Katherine C Du,Manik Kuchroo,Daniel B Burkhardt,Alexander Tong,Gary Israel,Marie E Robert,Stuart P Weisberg,Nancy Kirkiles-Smith,Angeliki M Stamatouli,Harriet M Kluger,Zoe Quandt,Arabella Young,Mei-Ling Yang,Mark J Mamula,Jordan S Pober,Mark S Anderson,Smita Krishnaswamy,Kevan C HeroldPubMed 35925682
- Journal of animal physiology and animal nutrition 107:518-5282022Effect of riboflavin on intestinal development and intestinal epithelial cell function of weaned piglets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Qin,Jing Zhou,Xia Xiong,Jing Huang,Jianzhong Li,Qiye Wang,Huansheng Yang,Yulong YinPubMed 35534939
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Expected | Tested | Expected |
| Dog | Predicted | Predicted | Predicted |
| Monkey | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/200 | Notes For unpurified use at 1/100 - 1/250. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info 1/200 | Notes For unpurified use at 1/100 - 1/250. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Pig, Monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/2000 | Notes For unpurified use at 1/10000. |
Species Human | Dilution info 1/2000 | Notes For unpurified use at 1/10000. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Pig, Monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes For unpurified use at 1/50 - 1/100. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Pig, Monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
PancreastatinStrongly inhibits glucose induced insulin release from the pancreas.CatestatinInhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed:15326220). Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed:15723172 and PubMed:24723458). Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed:21214543). Acts as a potent scavenger of free radicals in vitro (PubMed:24723458). May play a role in the regulation of cardiac function and blood pressure (PubMed:18541522).SerpininRegulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.
Alternative names
Chromogranin-A, CgA, Pituitary secretory protein I, SP-I, CHGA
Recommended products
Rabbit Polyclonal Chromogranin A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 35 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Chromogranin A (CgA) sometimes called chromagranin A is a glycoprotein with a molecular weight of approximately 49–52 kDa. It is highly expressed in the secretory granules of neuroendocrine cells present in tissues such as the adrenal medulla pancreas and endocrine cells of the gastrointestinal tract. CgA identified as part of a family including chromogranin B and chromogranin C functions mechanically as a precursor to various bioactive peptides. This translates into its involvement in the storage and release of hormones and peptides within vesicles.
- Biological function summary
Chromogranin A assists in the regulation of secretory pathways across multiple neuroendocrine systems. It plays a role in hormone storage conversion to active peptides and regulates the exocytosis of hormones. CgA acts within a complex contributing to secretory granule biogenesis and influencing intravesicular acidity. As a regulatory component the exact physiological roles are diverse impacting cardiovascular metabolic and immunological processes.
- Pathways
Chromogranin A is involved in pathways such as the catecholamine synthesis and release pathway and the serotonergic pathway. Within these pathways it interacts with proteins like secretogranin II and parathyroid hormone (PTH). These interactions modulate neurotransmitter storage and secretion across various systems. CgA's bioactive peptides also contribute to modulating physiological processes like vasoconstriction and insulin regulation linking it closely with these signaling cascades.
- Associated diseases and disorders
Chromogranin A is a known marker for neuroendocrine tumors where its levels in blood may increase due to tumor activity. It links to diseases like pheochromocytoma and carcinoid tumors where improper regulation of CgA-associated pathways occurs. Additionally its connection to parathyroid disorders through the PTH pathway illustrates its broader implication in endocrine pathophysiology. CgA serves as an invaluable diagnostic and prognostic tool in these disease states and supports the development of targeted therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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8 product images
This image is courtesy of a customer review submitted by Carl Hobbs, King's College London, United KingdomImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody (ab45179)
Unpurified ab45179 staining Chromogranin A in pig small intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/750 in blocking buffer) for 2 hours at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
Western blot - Anti-Chromogranin A antibody (ab45179)
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Chromogranin A antibody (ab45179) at 1/5000 dilution
All lanes: SH-SY5Y cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 86 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin A antibody (ab45179)
ab45179 staining Chromogranin A in SHSY5Y cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab45179 at 5µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody (ab45179)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human adenocarcinoma of pancreas tissue labelling Chromogranin A with purified ab45179 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-Chromogranin A antibody (ab45179)
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Chromogranin A antibody (ab45179) at 1/1000 dilution
All lanes: PC-12 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 86 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody (ab45179)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse pancreas tissue labelling Chromogranin A with purified ab45179 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-Chromogranin A antibody (ab45179)
All lanes: Western blot - Anti-Chromogranin A antibody (ab45179) at 1/10000 dilution
All lanes: PC-12 cell lysate at 10 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 50 kDa
Observed band size: 86 kDa
This image is courtesy of a customer review submitted by Carl Hobbs, King's College London, United KingdomImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody (ab45179)
Chromogranin A staining on monkey pancreas sections. The sections were subjected to heat mediated epitope retrieval using citric acid (pH 6) and blocked using 1% BSA for 10 mins at 21°C. Unpurifeid ab45179 was used at a dilution of 1/750 using TBS/BSA/Azide for 2 hours at 21°C. (Red arrowheads indicate extra Islet positive cells).
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