AB269806

Anti-Chromogranin A antibody [CGA/413] - BSA and Azide free

Name: Anti-Chromogranin A antibody [CGA/413] - BSA and Azide free (ab269806) | Abcam
Brand: Abcam Limited
SKU: AB269806
Price: 905 USD
Availability: InStock

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Mouse Monoclonal Chromogranin A antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CHGA.

View Alternative Names

Chromogranin-A, CgA, Pituitary secretory protein I, SP-I, CHGA

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [CGA/413] - BSA and Azide free (AB269806)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [CGA/413] - BSA and Azide free (AB269806)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adrenal gland tissue labeling Chromogranin A with ab220189 at 0.5 μg/ml.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220189)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [CGA/413] - BSA and Azide free (AB269806)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling Chromogranin A with ab220189 at 0.5 μg/ml.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220189)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [CGA/413] - BSA and Azide free (AB269806)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human parathyroid tissue labeling Chromogranin A with ab220189 at 0.5 μg/ml.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220189)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [CGA/413] - BSA and Azide free (AB269806)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pheochromocytoma tissue labeling Chromogranin A with ab220189 at 0.5 μg/ml.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220189)

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CGA/413

Isotype

IgG2b

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CHGA.

P10645

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.25-0.5 µg/mL", "IHCP-species-notes": "<p>For 30 minutes at RT.</p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "0.25-0.5 µg/mL", "IHCP-species-notes": "<p>For 30 minutes at RT.</p>" } } }

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Product details

ab269806 is a carrier free version of ab220189.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

ab22189 is purified from Bioreactor Concentrate by Protein A/G column chromatography.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Chromogranin A (CgA) sometimes called chromagranin A is a glycoprotein with a molecular weight of approximately 49–52 kDa. It is highly expressed in the secretory granules of neuroendocrine cells present in tissues such as the adrenal medulla pancreas and endocrine cells of the gastrointestinal tract. CgA identified as part of a family including chromogranin B and chromogranin C functions mechanically as a precursor to various bioactive peptides. This translates into its involvement in the storage and release of hormones and peptides within vesicles.

Biological function summary

Chromogranin A assists in the regulation of secretory pathways across multiple neuroendocrine systems. It plays a role in hormone storage conversion to active peptides and regulates the exocytosis of hormones. CgA acts within a complex contributing to secretory granule biogenesis and influencing intravesicular acidity. As a regulatory component the exact physiological roles are diverse impacting cardiovascular metabolic and immunological processes.

Pathways

Chromogranin A is involved in pathways such as the catecholamine synthesis and release pathway and the serotonergic pathway. Within these pathways it interacts with proteins like secretogranin II and parathyroid hormone (PTH). These interactions modulate neurotransmitter storage and secretion across various systems. CgA's bioactive peptides also contribute to modulating physiological processes like vasoconstriction and insulin regulation linking it closely with these signaling cascades.

Chromogranin A is a known marker for neuroendocrine tumors where its levels in blood may increase due to tumor activity. It links to diseases like pheochromocytoma and carcinoid tumors where improper regulation of CgA-associated pathways occurs. Additionally its connection to parathyroid disorders through the PTH pathway illustrates its broader implication in endocrine pathophysiology. CgA serves as an invaluable diagnostic and prognostic tool in these disease states and supports the development of targeted therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pancreastatin. Strongly inhibits glucose induced insulin release from the pancreas.. Catestatin. Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed : 15326220). Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed : 15723172, PubMed : 24723458). Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed : 21214543). Acts as a potent scavenger of free radicals in vitro (PubMed : 24723458). May play a role in the regulation of cardiac function and blood pressure (PubMed : 18541522).. Serpinin. Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.

See full target information CHGA

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