Rabbit Recombinant Monoclonal Chromogranin A antibody. Carrier free. Suitable for Protein Array, IHC-P and reacts with Recombinant full length protein - Human, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CHGA.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [CHGA/1731R] - BSA and Azide free (AB237820), expandable thumbnail](https://content.abcam.com/products/images/chromogranin-a-antibody-chga-1731r-bsa-and-azide-free-ab237820--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100190.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [CHGA/1731R] - BSA and Azide free (AB237820)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [CHGA/1731R] - BSA and Azide free (AB237820), expandable thumbnail](https://content.abcam.com/products/images/chromogranin-a-antibody-chga-1731r-bsa-and-azide-free-ab237820--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84376.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [CHGA/1731R] - BSA and Azide free (AB237820)")
![Protein Array - Anti-Chromogranin A antibody [CHGA/1731R] - BSA and Azide free (AB237820), expandable thumbnail](https://content.abcam.com/products/images/chromogranin-a-antibody-chga-1731r-bsa-and-azide-free-ab237820--protein-array-img141455.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Chromogranin A antibody [CHGA/1731R] - BSA and Azide free (AB237820)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human CHGA. Database link P10645
Reactivity data
Select an application| Protein Array | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Rat | Not recommended | Not recommended |
| Recombinant full length protein - Human | Tested | Not recommended |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Incubate with primary antibody for 30 mins at RT. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Incubate with primary antibody for 30 mins at RT. |
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Target data
Function
PancreastatinStrongly inhibits glucose induced insulin release from the pancreas.CatestatinInhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed:15326220). Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed:15723172 and PubMed:24723458). Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed:21214543). Acts as a potent scavenger of free radicals in vitro (PubMed:24723458). May play a role in the regulation of cardiac function and blood pressure (PubMed:18541522).SerpininRegulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.
Alternative names
Chromogranin-A, CgA, Pituitary secretory protein I, SP-I, CHGA
Recommended products
Rabbit Recombinant Monoclonal Chromogranin A antibody. Carrier free. Suitable for Protein Array, IHC-P and reacts with Recombinant full length protein - Human, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CHGA.
Alternative names
Chromogranin-A, CgA, Pituitary secretory protein I, SP-I, CHGA
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human CHGA. Database link P10645
- Carrier free
Yes
- Clone number
CHGA/1731R
- Purification technique
Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from bioreactor concentrate.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
ab237820 is the carrier-free version of Anti-Chromogranin A antibody [CHGA/1731R] ab237979.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Chromogranin A (CgA) sometimes called chromagranin A is a glycoprotein with a molecular weight of approximately 49–52 kDa. It is highly expressed in the secretory granules of neuroendocrine cells present in tissues such as the adrenal medulla pancreas and endocrine cells of the gastrointestinal tract. CgA identified as part of a family including chromogranin B and chromogranin C functions mechanically as a precursor to various bioactive peptides. This translates into its involvement in the storage and release of hormones and peptides within vesicles.
- Biological function summary
Chromogranin A assists in the regulation of secretory pathways across multiple neuroendocrine systems. It plays a role in hormone storage conversion to active peptides and regulates the exocytosis of hormones. CgA acts within a complex contributing to secretory granule biogenesis and influencing intravesicular acidity. As a regulatory component the exact physiological roles are diverse impacting cardiovascular metabolic and immunological processes.
- Pathways
Chromogranin A is involved in pathways such as the catecholamine synthesis and release pathway and the serotonergic pathway. Within these pathways it interacts with proteins like secretogranin II and parathyroid hormone (PTH). These interactions modulate neurotransmitter storage and secretion across various systems. CgA's bioactive peptides also contribute to modulating physiological processes like vasoconstriction and insulin regulation linking it closely with these signaling cascades.
- Associated diseases and disorders
Chromogranin A is a known marker for neuroendocrine tumors where its levels in blood may increase due to tumor activity. It links to diseases like pheochromocytoma and carcinoid tumors where improper regulation of CgA-associated pathways occurs. Additionally its connection to parathyroid disorders through the PTH pathway illustrates its broader implication in endocrine pathophysiology. CgA serves as an invaluable diagnostic and prognostic tool in these disease states and supports the development of targeted therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [CHGA/1731R] - BSA and Azide free (ab237820), expandable thumbnail](https://content.abcam.com/products/images/chromogranin-a-antibody-chga-1731r-bsa-and-azide-free-ab237820--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100190.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [CHGA/1731R] - BSA and Azide free (ab237820)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [CHGA/1731R] - BSA and Azide free (ab237820)
Formalin-fixed, paraffin-embedded human parathyroid tissue stained for Chromogranin A with Anti-Chromogranin A antibody [CHGA/1731R] ab237979 at 1 μg/ml in immunohistochemical analysis.
This data was produced with Anti-Chromogranin A antibody [CHGA/1731R] ab237979, the same antibody in a different formulation with BSA and Azide.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [CHGA/1731R] - BSA and Azide free (ab237820), expandable thumbnail](https://content.abcam.com/products/images/chromogranin-a-antibody-chga-1731r-bsa-and-azide-free-ab237820--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84376.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [CHGA/1731R] - BSA and Azide free (ab237820)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [CHGA/1731R] - BSA and Azide free (ab237820)
Formalin-fixed, paraffin-embedded human pancreas tissue stained for Chromogranin A with Anti-Chromogranin A antibody [CHGA/1731R] ab237979 at 1 μg/ml in immunohistochemical analysis.
This data was produced with Anti-Chromogranin A antibody [CHGA/1731R] ab237979, the same antibody in a different formulation with BSA and Azide.
![Protein Array - Anti-Chromogranin A antibody [CHGA/1731R] - BSA and Azide free (ab237820), expandable thumbnail](https://content.abcam.com/products/images/chromogranin-a-antibody-chga-1731r-bsa-and-azide-free-ab237820--protein-array-img141455.jpg "Protein Array - Anti-Chromogranin A antibody [CHGA/1731R] - BSA and Azide free (ab237820)")
Protein Array - Anti-Chromogranin A antibody [CHGA/1731R] - BSA and Azide free (ab237820)
This data was produced with Anti-Chromogranin A antibody [CHGA/1731R] ab237979, the same antibody in a different formulation with BSA and Azide.
Anti-Chromogranin A antibody [CHGA/1731R] ab237979 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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