AB52983

Anti-Chromogranin A antibody [EP1031Y]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal Chromogranin A antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 4 publications.

View Alternative Names

Chromogranin-A, CgA, Pituitary secretory protein I, SP-I, CHGA

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EP1031Y] (AB52983)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EP1031Y] (AB52983)

ab52983 at a 1/250 dilution staining Human Chromogranin A in Pancreas using Immunohistochemistry Paraffin Emebedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Chromogranin A antibody [EP1031Y] (AB52983)

All lanes:

Western blot - Anti-Chromogranin A antibody [EP1031Y] (ab52983) at 1/1000000 dilution

All lanes:

PC12 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP labelled at 1/2000 dilution

Predicted band size: 50 kDa

Observed band size: 80-90 kDa

false

OI-RD Scanning - Anti-Chromogranin A antibody [EP1031Y] (AB52983)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Chromogranin A antibody [EP1031Y] (AB52983)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-Chromogranin A antibody [EP1031Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1031Y

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Chromogranin A (CgA) sometimes called chromagranin A is a glycoprotein with a molecular weight of approximately 49–52 kDa. It is highly expressed in the secretory granules of neuroendocrine cells present in tissues such as the adrenal medulla pancreas and endocrine cells of the gastrointestinal tract. CgA identified as part of a family including chromogranin B and chromogranin C functions mechanically as a precursor to various bioactive peptides. This translates into its involvement in the storage and release of hormones and peptides within vesicles.

Biological function summary

Chromogranin A assists in the regulation of secretory pathways across multiple neuroendocrine systems. It plays a role in hormone storage conversion to active peptides and regulates the exocytosis of hormones. CgA acts within a complex contributing to secretory granule biogenesis and influencing intravesicular acidity. As a regulatory component the exact physiological roles are diverse impacting cardiovascular metabolic and immunological processes.

Pathways

Chromogranin A is involved in pathways such as the catecholamine synthesis and release pathway and the serotonergic pathway. Within these pathways it interacts with proteins like secretogranin II and parathyroid hormone (PTH). These interactions modulate neurotransmitter storage and secretion across various systems. CgA's bioactive peptides also contribute to modulating physiological processes like vasoconstriction and insulin regulation linking it closely with these signaling cascades.

Chromogranin A is a known marker for neuroendocrine tumors where its levels in blood may increase due to tumor activity. It links to diseases like pheochromocytoma and carcinoid tumors where improper regulation of CgA-associated pathways occurs. Additionally its connection to parathyroid disorders through the PTH pathway illustrates its broader implication in endocrine pathophysiology. CgA serves as an invaluable diagnostic and prognostic tool in these disease states and supports the development of targeted therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pancreastatin. Strongly inhibits glucose induced insulin release from the pancreas.. Catestatin. Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed : 15326220). Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed : 15723172, PubMed : 24723458). Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed : 21214543). Acts as a potent scavenger of free radicals in vitro (PubMed : 24723458). May play a role in the regulation of cardiac function and blood pressure (PubMed : 18541522).. Serpinin. Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.

See full target information CHGA

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

BMC surgery 21:340 PubMed34496808

2021

Pitfalls in the management of subglottic paragangliomas at unusual location: a case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Juanjuan Hu,Haiyang Wang,Jianli Chen,Xuelin Pan,Di Deng,Lufang Zhuo,Shixi Liu,Maiyue He,Fei Chen,Hui Yang

Journal of cellular physiology 236:7745-7758 PubMed34061983

2021

Multiple roles for peptidylglycine α-amidating monooxygenase in the response to hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Vishwanatha K S Rao,Betty A Eipper,Richard E Mains

Nature genetics 50:1122-1131 PubMed30054598

2018

Type 2 diabetes risk alleles in PAM impact insulin release from human pancreatic β-cells.

Applications

Unspecified application

Species

Unspecified reactive species

Soren K Thomsen,Anne Raimondo,Benoit Hastoy,Shahana Sengupta,Xiao-Qing Dai,Austin Bautista,Jenny Censin,Anthony J Payne,Mahesh M Umapathysivam,Aliya F Spigelman,Amy Barrett,Christopher J Groves,Nicola L Beer,Jocelyn E Manning Fox,Mark I McCarthy,Anne Clark,Anubha Mahajan,Patrik Rorsman,Patrick E MacDonald,Anna L Gloyn

The Journal of biological chemistry 290:24891-901 PubMed26296884

2015

Striking Oxygen Sensitivity of the Peptidylglycine α-Amidating Monooxygenase (PAM) in Neuroendocrine Cells.

Applications

Species

Human

Peter D Simpson,Betty A Eipper,Maximiliano J Katz,Lautaro Gandara,Pablo Wappner,Roman Fischer,Emma J Hodson,Peter J Ratcliffe,Norma Masson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Chromogranin A antibody [EP1031Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EP1031Y] (AB52983)

Western blot - Anti-Chromogranin A antibody [EP1031Y] (AB52983)

OI-RD Scanning - Anti-Chromogranin A antibody [EP1031Y] (AB52983)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise