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Rabbit Recombinant Monoclonal Chromogranin A antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EP1031Y] - BSA and Azide free (AB247345), expandable thumbnail
  • Western blot - Anti-Chromogranin A antibody [EP1031Y] - BSA and Azide free (AB247345), expandable thumbnail
  • OI-RD Scanning - Anti-Chromogranin A antibody [EP1031Y] - BSA and Azide free (AB247345), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFFlow CytWB
Human
Tested
Not recommended
Not recommended
Expected
Rat
Expected
Not recommended
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Pancreastatin. Strongly inhibits glucose induced insulin release from the pancreas. Catestatin. Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed:15326220). Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed:15723172, PubMed:24723458). Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed:21214543). Acts as a potent scavenger of free radicals in vitro (PubMed:24723458). May play a role in the regulation of cardiac function and blood pressure (PubMed:18541522). Serpinin. Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.

Alternative names

Chromogranin-A, CgA, Pituitary secretory protein I, SP-I, CHGA

Recommended products

Rabbit Recombinant Monoclonal Chromogranin A antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EP1031Y
Purification technique
Affinity purification Protein A
Dissociation constant
6.9 x 10-12 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab247345 is the carrier-free version of Anti-Chromogranin A antibody [EP1031Y] ab52983.

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Chromogranin A (CgA) sometimes called chromagranin A is a glycoprotein with a molecular weight of approximately 49–52 kDa. It is highly expressed in the secretory granules of neuroendocrine cells present in tissues such as the adrenal medulla pancreas and endocrine cells of the gastrointestinal tract. CgA identified as part of a family including chromogranin B and chromogranin C functions mechanically as a precursor to various bioactive peptides. This translates into its involvement in the storage and release of hormones and peptides within vesicles.

Biological function summary

Chromogranin A assists in the regulation of secretory pathways across multiple neuroendocrine systems. It plays a role in hormone storage conversion to active peptides and regulates the exocytosis of hormones. CgA acts within a complex contributing to secretory granule biogenesis and influencing intravesicular acidity. As a regulatory component the exact physiological roles are diverse impacting cardiovascular metabolic and immunological processes.

Pathways

Chromogranin A is involved in pathways such as the catecholamine synthesis and release pathway and the serotonergic pathway. Within these pathways it interacts with proteins like secretogranin II and parathyroid hormone (PTH). These interactions modulate neurotransmitter storage and secretion across various systems. CgA's bioactive peptides also contribute to modulating physiological processes like vasoconstriction and insulin regulation linking it closely with these signaling cascades.

Associated diseases and disorders

Chromogranin A is a known marker for neuroendocrine tumors where its levels in blood may increase due to tumor activity. It links to diseases like pheochromocytoma and carcinoid tumors where improper regulation of CgA-associated pathways occurs. Additionally its connection to parathyroid disorders through the PTH pathway illustrates its broader implication in endocrine pathophysiology. CgA serves as an invaluable diagnostic and prognostic tool in these disease states and supports the development of targeted therapies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EP1031Y] - BSA and Azide free (ab247345), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EP1031Y] - BSA and Azide free (ab247345)

    This data was developed using Anti-Chromogranin A antibody [EP1031Y] ab52983, the same antibody clone in a different buffer formulation.Anti-Chromogranin A antibody [EP1031Y] ab52983, at a 1/250 dilution, staining Human Chromogranin A in Pancreas using Immunohistochemistry, Paraffin Emebedded tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Chromogranin A antibody [EP1031Y] - BSA and Azide free (ab247345), expandable thumbnail

    Western blot - Anti-Chromogranin A antibody [EP1031Y] - BSA and Azide free (ab247345)

    This data was developed using Anti-Chromogranin A antibody [EP1031Y] ab52983, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Chromogranin A antibody [EP1031Y] (Anti-Chromogranin A antibody [EP1031Y] ab52983) at 1/1000000 dilution

    All lanes: PC12 cell lysate at 10 µg

    Secondary

    All lanes: goat anti-rabbit HRP labelled at 1/2000 dilution

    Predicted band size: 50 kDa

    Observed band size: 80-90 kDa

  • OI-RD Scanning - Anti-Chromogranin A antibody [EP1031Y] - BSA and Azide free (ab247345), expandable thumbnail

    OI-RD Scanning - Anti-Chromogranin A antibody [EP1031Y] - BSA and Azide free (ab247345)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com