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Rabbit Recombinant Monoclonal Chromogranin A antibody. Suitable for WB, ICC/IF, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat samples. Cited in 2 publications.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin A antibody [EPR22537-248] (AB254322), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-248] (AB254322), expandable thumbnail
  • Western blot - Anti-Chromogranin A antibody [EPR22537-248] (AB254322), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Chromogranin A antibody [EPR22537-248] (AB254322), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-248] (AB254322), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-FrFlow Cyt (Intra)IHC-P
Mouse
Not recommended
Tested
Tested
Tested
Tested
Tested
Rat
Not recommended
Tested
Tested
Tested
Expected
Tested

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

-

Species

Rat

Dilution info

1/100

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Species

Rat

Dilution info

1/500

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Tested
Tested

Species

Mouse

Dilution info

1/60

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

PancreastatinStrongly inhibits glucose induced insulin release from the pancreas.CatestatinInhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist. Can induce mast cell migration, degranulation and production of cytokines and chemokines.SerpininRegulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation (PubMed:21436258). Pyroglutaminated (pGlu)-serpinin exerts an antiapoptotic effect on cells exposed to oxidative stress (PubMed:21537909).

Alternative names

Chromogranin-A, CgA, Chga

Recommended products

Rabbit Recombinant Monoclonal Chromogranin A antibody. Suitable for WB, ICC/IF, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat samples. Cited in 2 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22537-248

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin A antibody [EPR22537-248] (ab254322), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin A antibody [EPR22537-248] (ab254322)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma cell line) cells labeling Chromogranin A with ab254322 at 1/100 dilution, followed by a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining in Neuro-2a cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-248] (ab254322), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-248] (ab254322)

    Immunohistochemical analysis of paraffin-embedded rat panceas tissue labeling Chromogranin A with ab254322 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat pancreas islet (PMID: 22412953) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254322 for 30 mins at RT.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-Chromogranin A antibody [EPR22537-248] (ab254322), expandable thumbnail

    Western blot - Anti-Chromogranin A antibody [EPR22537-248] (ab254322)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lane 1: 15 seconds; Lane 2: 3.25 seconds; Lane 3:26 seconds; Lane 4: 3.25 seconds; Lane 5: 15 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 14734658, 21052719).

    All lanes: Western blot - Anti-Chromogranin A antibody [EPR22537-248] (ab254322) at 1/1000 dilution

    Lane 1: Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 20 µg

    Lane 2: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg

    Lane 3: Mouse serum at 20 µg

    Lane 4: Mouse adrenal gland lysate at 20 µg

    Lane 5: Rat adrenal gland lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 50 kDa

    Observed band size: 20 kDa, 75 kDa

  • Flow Cytometry (Intracellular) - Anti-Chromogranin A antibody [EPR22537-248] (ab254322), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Chromogranin A antibody [EPR22537-248] (ab254322)

    Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methanol-permeabilized Neuro-2a (mouse neuroblastoma cell line) cells labeling Chromogranin A with ab254322 at 1/60 (red) compared with Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-248] (ab254322), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-248] (ab254322)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Chromogranin A with ab254322 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse pancreas islet (PMID: 22412953) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254322 for 30 mins at RT.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Frozen sections) - Anti-Chromogranin A antibody [EPR22537-248] (ab254322), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Chromogranin A antibody [EPR22537-248] (ab254322)

    Frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat colon tissue labeling Chromogranin A with ab254322 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/1000 dilution (green). Positive staining on enteroendocrine cells of mouse colon (PMID: 27287799) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin A antibody [EPR22537-248] (ab254322), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin A antibody [EPR22537-248] (ab254322)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell line) cells labeling Chromogranin A with ab254322 at 1/100 dilution, followed by a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining in PC-12 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Chromogranin A antibody [EPR22537-248] (ab254322), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Chromogranin A antibody [EPR22537-248] (ab254322)

    Frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse colon tissue labeling Chromogranin A with ab254322 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/1000 dilution (green). Positive staining on enteroendocrine cells of mouse colon (PMID: 27287799). is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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