Anti-Chromogranin A antibody [EPR22537-249]

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Chromogranin A antibody [EPR22537-249] (AB254557)

Fluorescence multiplex immunohistochemical analysis of the human stomach (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-Chromogranin A (ab254557, gray; Opal™690), anti-MUC-6 (ab223846, green; Opal™520) and anti-Rab3D (ab128997, red; Opal™570) on human stomach. Panel B : anti-MUC-6 stained on mucous neck cells. Panel C : anti-Chromogranin A stained on neuroendocrine cells. Panel D : anti-Rab3D stained on Chief cells. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining : in the order of ab254557 (1/5000), ab223846 (1/1000), and ab128997 (1/10000) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] (AB254557)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Chromogranin A with ab254557 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human pancreas islet (PMID : 22412953) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab254557 for 10 mins at RT.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] (AB254557)

Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue labeling Chromogranin A with ab254557 at 1/5000 dilution, followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human adrenal medulla (PMID : 15615849) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab254557 for 10 mins at RT.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Chromogranin A antibody [EPR22537-249] (AB254557)

Fluorescence multiplex immunohistochemical analysis of the human duodenum (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-liver FABP (ab240401, gray; Opal™690), anti-Lysozyme (ab242430, green; Opal™520) and anti-Chromogranin A (ab254557, red; Opal™570) on human duodenum. Panel B : anti-liver FABP stained on enterocytes. Panel C : anti-Lysozyme stained on Paneth cells. Panel D : anti-Chromogranin A stained on neuroendocrine cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining : in the order of ab240401 (1/8000 dilution), ab242430 (1 : 250 dilution), and ab254557 (1/5000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] (AB254557)

Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling Chromogranin A with ab254557 at 1/5000 dilution, followed by a rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse adrenal medulla (PMID : 15615849) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab254557 for 10 mins at RT.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] (AB254557)

Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Chromogranin A with ab254557 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Strong staining on rat adrenal medulla (PMID : 15615849) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab254557 for 10 mins at RT.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] (AB254557)

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Chromogranin A with ab254557 at 1/5000 dilution, followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat pancreas islet (PMID : 22412953) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab254557 for 10 mins at RT.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] (AB254557)

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Chromogranin A with ab254557 at 1/5000 dilution, followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse pancreas islet (PMID : 22412953) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab254557 for 10 mins at RT.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Chromogranin A antibody [EPR22537-249] (AB254557)

Immunohistochemical analysis of frozen section of rat colon tissue labeling Chromogranin A with ab254557 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining on enteroendocrine cells of colon (PMID : 27287799) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Chromogranin A antibody [EPR22537-249] (AB254557)

Immunohistochemical analysis of frozen section of mouse colon tissue labeling Chromogranin A with ab254557 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining on enteroendocrine cells of colon (PMID : 27287799) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• WB

Unknown

Western blot - Anti-Chromogranin A antibody [EPR22537-249] (AB254557)

Blocking and dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 14734658, 21052719, 24260544).

All lanes:

Western blot - Anti-Chromogranin A antibody [EPR22537-249] (ab254557) at 1/1000 dilution

All lanes:

Human adrenal gland tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 75 kDa

false

Exposure time: 48s

• WB

Unknown

Western blot - Anti-Chromogranin A antibody [EPR22537-249] (AB254557)

Blocking and dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 14734658, 21052719,24260544).

All lanes:

Western blot - Anti-Chromogranin A antibody [EPR22537-249] (ab254557) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 20 µg

Lane 2:

PC-12 (rat adrenal gland pheochromocytoma cell line0 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 50 kDa

Observed band size: 100 kDa,20 kDa,75 kDa,85 kDa

false

Exposure time: 8s