Name: Anti-Chromogranin A antibody [RM1025]
SKU: ab283265
Rating: 5 (1 reviews)

Anti-Chromogranin A antibody [RM1025] (ab283265) is a rabbit recombinant multiclonal antibody that is used to detect Chromogranin A in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF, ELISA. Suitable for Human, Mouse, Rat samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

Images

Western blot - Anti-Chromogranin A antibody [RM1025] (AB283265), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Multiclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	ELISA	WB	ICC/IF	IHC-Fr	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested	Tested	Expected	Tested
Mouse	Tested	Tested	Tested	Tested	Tested	Tested	Tested
Rat	Tested	Expected	Expected	Tested	Expected	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Human	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -
Species Human	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 0.125 µg/mL	Notes -
Species Human	Dilution info 0.125 µg/mL	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes -
Species Human	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -
Species Rat	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -
Species Human	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Target data

See full target information CHGA

Function

Pancreastatin. Strongly inhibits glucose induced insulin release from the pancreas. Catestatin. Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist (PubMed:15326220). Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa (PubMed:15723172, PubMed:24723458). Can induce mast cell migration, degranulation and production of cytokines and chemokines (PubMed:21214543). Acts as a potent scavenger of free radicals in vitro (PubMed:24723458). May play a role in the regulation of cardiac function and blood pressure (PubMed:18541522). Serpinin. Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.

Additional Targets

Chromogranin-A

Alternative names

Chromogranin-A, CgA, Pituitary secretory protein I, SP-I, CHGA

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Multiclonal
Immunogens: The exact immunogen used to generate this antibody is proprietary information.
Clone number: RM1025
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-Chromogranin A antibody [RM1025] (ab283265) is a rabbit recombinant recombinant multiclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA, in Human, Mouse, Rat samples.

What is the molecular weight of Chromogranin A?

Anti-Chromogranin A [RM1025] (ab283265) specifically detects a band for Chromogranin A (UniProt: P10645) at a molecular weight of 50kDa.

Recommended positive controls

WB: Human adrenal gland, SH-SY5Y, Neuro-2a, Mouse adrenal gland, Rat adrenal gland, PC-12 lysates.IHC-P: Human pancreas, Human stomach, Mouse pancreas, Mouse adrenal gland, Rat pancreas, Rat adrenal gland tissues.IHC-Fr: Mouse colon, Rat colon tissues.ICC/IF: SH-SY5Y, Neuro-2a cells.Flow Cyt (Intra): SH-SY5Y, Neuro-2a cells.IP: SH-SY5Y, Neuro-2a cells.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products

We have a range of other formats of antibody clone [RM1025] also available for your convenience:
ab283265, Carrier free - ab283286

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Chromogranin A (CgA) sometimes called chromagranin A is a glycoprotein with a molecular weight of approximately 49–52 kDa. It is highly expressed in the secretory granules of neuroendocrine cells present in tissues such as the adrenal medulla pancreas and endocrine cells of the gastrointestinal tract. CgA identified as part of a family including chromogranin B and chromogranin C functions mechanically as a precursor to various bioactive peptides. This translates into its involvement in the storage and release of hormones and peptides within vesicles.

Biological function summary

Chromogranin A assists in the regulation of secretory pathways across multiple neuroendocrine systems. It plays a role in hormone storage conversion to active peptides and regulates the exocytosis of hormones. CgA acts within a complex contributing to secretory granule biogenesis and influencing intravesicular acidity. As a regulatory component the exact physiological roles are diverse impacting cardiovascular metabolic and immunological processes.

Pathways

Chromogranin A is involved in pathways such as the catecholamine synthesis and release pathway and the serotonergic pathway. Within these pathways it interacts with proteins like secretogranin II and parathyroid hormone (PTH). These interactions modulate neurotransmitter storage and secretion across various systems. CgA's bioactive peptides also contribute to modulating physiological processes like vasoconstriction and insulin regulation linking it closely with these signaling cascades.

Associated diseases and disorders

Chromogranin A is a known marker for neuroendocrine tumors where its levels in blood may increase due to tumor activity. It links to diseases like pheochromocytoma and carcinoid tumors where improper regulation of CgA-associated pathways occurs. Additionally its connection to parathyroid disorders through the PTH pathway illustrates its broader implication in endocrine pathophysiology. CgA serves as an invaluable diagnostic and prognostic tool in these disease states and supports the development of targeted therapies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

17 product images

Western blot - Anti-Chromogranin A antibody [RM1025] (ab283265)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: Jurkat.
The molecular weight observed is consistent with what has been described in the literature (PMID: 14734658, 21052719).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 min
All lanes: Western blot - Anti-Chromogranin A antibody [RM1025] (ab283265) at 1/1000 dilution
Lane 1: Human adrenal gland lysate at 20 µg
Lane 2: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 20 µg
Lane 3: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg
Lane 4: Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 50 kDa
Observed band size: 20 kDa, 75 kDa
Western blot - Anti-Chromogranin A antibody [RM1025] (ab283265)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: NIH/3T3. (PMID: 7615829)
Exposure time: Lane 1-2: 5 seconds Lane 3-5: 92 seconds
All lanes: Western blot - Anti-Chromogranin A antibody [RM1025] (ab283265) at 1/1000 dilution
Lane 1: Mouse adrenal gland lysate at 20 µg
Lane 2: Rat adrenal gland lysate at 20 µg
Lane 3: Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Lane 4: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 5: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 50 kDa
Observed band size: 20 kDa, 75 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [RM1025] (ab283265)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labelling Chromogranin A with ab283265 at 1/5000 dilution (0.107 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on the islet in human pancreas. The section was incubated with ab283265 overnight at 4℃. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [RM1025] (ab283265)
Immunohistochemical analysis of paraffin-embedded human stomach tissue labelling Chromogranin A with ab283265 at 1/5000 dilution (0.107 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on the endocrine cells in human stomach. The section was incubated with ab283265 overnight at 4℃. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [RM1025] (ab283265)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labelling Chromogranin A with ab283265 at 1/5000 dilution (0.107 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on the islet in mouse pancreas. The section was incubated with ab283265 overnight at 4℃. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [RM1025] (ab283265)
Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labelling Chromogranin A with ab283265 at 1/5000 dilution (0.107 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining in the mouse adrenal gland. The section was incubated with ab283265 overnight at 4℃. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [RM1025] (ab283265)
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labelling Chromogranin A with ab283265 at 1/5000 dilution (0.107 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on the islet in rat pancreas. The section was incubated with ab283265 overnight at 4℃. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [RM1025] (ab283265)
Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labelling Chromogranin A with ab283265 at 1/5000 dilution (0.107 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining in the rat adrenal gland. The section was incubated with ab283265 overnight at 4℃. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0)
Immunohistochemistry (Frozen sections) - Anti-Chromogranin A antibody [RM1025] (ab283265)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon (fresh) tissue labeling Chromogranin A with ab283265 at 1/500 dilution (1.074 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 μg/ml) (Green). Positive satining on mouse colon is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed.
Immunohistochemistry (Frozen sections) - Anti-Chromogranin A antibody [RM1025] (ab283265)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat colon (fresh) tissue labeling Chromogranin A with ab283265 at 1/500 dilution (1.074 μg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 μg/ml) (Green). Positive staining on rat colon is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed.
Immunoprecipitation - Anti-Chromogranin A antibody [RM1025] (ab283265)
Chromogranin A was immunoprecipitated from 0.35 mg SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate with ab283265 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283265 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate 10ug
Lane 2: ab283265 IP in SH-SY5Y whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab283265 in SH-SY5Y whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds
Lower bands could be cleavage fragment. (PMID: 17991725)
All lanes: Immunoprecipitation - Anti-Chromogranin A antibody [RM1025] (ab283265)
Predicted band size: 50 kDa
Observed band size: 75 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin A antibody [RM1025] (ab283265)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labelling Chromogranin A with ab283265 at 1/50 dilution (10.74 μg/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution (2 μg/ml) (Green). Confocal image showing cytoplasmic staining in subsets of SH-SY5Y cells.
Negative control: Jurkat.
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (2.5 μg/ml) (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed.
Immunoprecipitation - Anti-Chromogranin A antibody [RM1025] (ab283265)
Chromogranin A was immunoprecipitated from 0.35 mg Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate with ab283265 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283265 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate 10ug
Lane 2: ab283265 IP in Neuro-2a whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab283265 in Neuro-2a whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 min
Lower bands could be cleavage fragment. (PMID: 17991725)
All lanes: Immunoprecipitation - Anti-Chromogranin A antibody [RM1025] (ab283265)
Predicted band size: 50 kDa
Observed band size: 75 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin A antibody [RM1025] (ab283265)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a (mouse neuroblastoma cell line) cells labelling Chromogranin A with ab283265 at 1/50 dilution (10.74 μg/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution (2 μg/ml) (Green). Confocal image showing cytoplasmic staining in of Neuro-2a cells.
Negative control: NIH/3T3 (PMID: 7615829).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (2.5 μg/ml) (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed.
Flow Cytometry (Intracellular) - Anti-Chromogranin A antibody [RM1025] (ab283265)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte / Left) and SH-SY5Y (Human neuroblastoma epithelial cell / Right) cells labelling Chromogranin A with ab283265 at 1/500 dilution (0.1 μg) compared with an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control: Jurkat.
Flow Cytometry (Intracellular) - Anti-Chromogranin A antibody [RM1025] (ab283265)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast / Left) and Neuro-2a (Mouse neuroblastoma neuroblast / Right) cells labelling Chromogranin A with ab283265 at 1/500 dilution (0.1 μg / Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 / Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control: NIH/3T3. (PMID: 7615829)
ELISA - Anti-Chromogranin A antibody [RM1025] (ab283265)
ELISA using ab283265 at varying antibody concentrations and antigen concentration at 1000 ng/ml. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (1/2500) was used as the secondary antibody.