AB12242

Anti-Chromogranin B antibody

Name: Anti-Chromogranin B antibody (ab12242) | Abcam
Brand: Abcam Limited
SKU: AB12242
Price: 545 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(11 Publications)

Rabbit Polyclonal Chromogranin B antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human CHGB aa 600 to C-terminus conjugated to Ovalbumin.

View Alternative Names

SCG1, CHGB, Secretogranin-1, Chromogranin-B, Secretogranin I, CgB, SgI

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin B antibody (AB12242)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin B antibody (AB12242)

ab12242(1 : 1000) staining Chromogranin B in human pancreas (IgG inset) using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of Chromogranin B in secretory compartments of the pancreas.
Insert panel depicts negative control (no primary antibody).
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX.
Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CHGB aa 600 to C-terminus conjugated to Ovalbumin. The exact immunogen used to generate this antibody is proprietary information.

P05060

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/2500 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/5000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/2500 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/2500 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Chromogranin B also known as secretogranin I is a member of the granin protein family and is sometimes referred to alongside chromogranin A and other chromogranins. Chromogranin B is a secretory protein with a known mass of approximately 80 kDa. This protein expresses primarily in neuroendocrine cells where it localizes to the secretory granules. Chromogranins including chromogranin B play a role in the formation and stabilization of secretory granules important for storing and releasing peptide hormones and neuropeptides.

Biological function summary

Chromogranin B functions beyond merely being a constituent of secretory granules. It plays a role in the regulation of neuroendocrine secretion processes and interacts with the chromogranin core complex. This complex is involved in the packaging and sorting of peptides and hormones within cells. Chromogranin B also exhibits roles in modulating calcium-binding dynamics which is critical for cellular signaling and hormone secretion. Unlike chromogranin A chromogranin B is less abundant but still significant for cellular processes.

Pathways

Chromogranin B prominently aligns with the secretory pathway associated with neuroendocrine tissues. This pathway involves several proteins like chromogranin A that often work alongside chromogranin B to influence hormone release. Another pathway where chromogranin B plays a role involves the regulation of catecholamine release vital for stress responses. This particular involvement with catecholamines links chromogranin B to its role in neurotransmitter regulation.

Chromogranin B shows an association with conditions such as neuroendocrine tumors and hypertension. In neuroendocrine tumors chromogranin B can serve as a biomarker since its levels often elevate in these conditions akin to chromogranin A. Hypertension studies suggest connections between chromogranin B and the dysregulation of catecholamine release impacting blood pressure regulation. Understanding the role of chromogranin B and its related proteins can help in diagnosing and potentially targeting these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides.

See full target information CHGB

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:4697 PubMed40393969

2025

ACSS2 mediates an epigenetic pathway to regulate β-cell adaptation during gestation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhang,Shuang He,Xi Wang,Xin Wang,Mao-Yang He,Xin-Xin Yu,Cheng-Ran Xu

Scientific reports 11:21203 PubMed34707216

2021

Endocytic BDNF secretion regulated by Vamp3 in astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jeongho Han,Sungryeong Yoon,Hyungju Park

BMC biology 19:47 PubMed33722214

2021

Single-cell morphological characterization of CRH neurons throughout the whole mouse brain.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Pu Hu,Qinghong Shan,Chuan Huang,Zhaohuan Huang,Peng Chen,Anan Li,Hui Gong,Jiang-Ning Zhou

eLife 8: PubMed31663849

2019

deficiency results in colitis susceptibility.

Applications

Unspecified application

Species

Unspecified reactive species

Luis F Sifuentes-Dominguez,Haiying Li,Ernesto Llano,Zhe Liu,Amika Singla,Ashish S Patel,Mahesh Kathania,Areen Khoury,Nicholas Norris,Jonathan J Rios,Petro Starokadomskyy,Jason Y Park,Purva Gopal,Qi Liu,Shuai Tan,Lillienne Chan,Theodora Ross,Steven Harrison,K Venuprasad,Linda A Baker,Da Jia,Ezra Burstein

The Journal of clinical investigation 127:4569-4582 PubMed29058688

2017

Small GTPase ARF6 controls VEGFR2 trafficking and signaling in diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Weiquan Zhu,Dallas S Shi,Jacob M Winter,Bianca E Rich,Zongzhong Tong,Lise K Sorensen,Helong Zhao,Yi Huang,Zhengfu Tai,Tara M Mleynek,Jae Hyuk Yoo,Christine Dunn,Jing Ling,Jake A Bergquist,Jackson R Richards,Amanda Jiang,Lisa A Lesniewski,M Elizabeth Hartnett,Diane M Ward,Alan L Mueller,Kirill Ostanin,Kirk R Thomas,Shannon J Odelberg,Dean Y Li

Cell reports 20:2480-2489 PubMed28877479

2017

The Prohormone VGF Regulates β Cell Function via Insulin Secretory Granule Biogenesis.

Applications

ICC/IF, WB

Species

Unspecified reactive species, Unspecified reactive species

Samuel B Stephens,Robert J Edwards,Masato Sadahiro,Wei-Jye Lin,Cheng Jiang,Stephen R Salton,Christopher B Newgard

PloS one 9:e85484 PubMed24454876

2014

RanBP9 overexpression accelerates loss of pre and postsynaptic proteins in the APΔE9 transgenic mouse brain.

Applications

ICC/IF, WB

Species

Mouse, Mouse

Hongjie Wang,Ruizhi Wang,Shaohua Xu,Madepalli K Lakshmana

Hormones & cancer 4:165-75 PubMed23361940

2013

Altered PTEN, ATRX, CHGA, CHGB, and TP53 expression are associated with aggressive VHL-associated pancreatic neuroendocrine tumors.

Applications

IHC-P

Species

Human

Allison B Weisbrod,Lisa Zhang,Meenu Jain,Stephanie Barak,Martha M Quezado,Electron Kebebew

The Journal of clinical investigation 122:3159-69 PubMed22922254

2012

Cerebrospinal fluid-based kinetic biomarkers of axonal transport in monitoring neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Patrizia Fanara,Po-Yin A Wong,Kristofor H Husted,Shanshan Liu,Victoria M Liu,Lori A Kohlstaedt,Timothy Riiff,Joan C Protasio,Drina Boban,Salena Killion,Maudi Killian,Lorrie Epling,Elisabeth Sinclair,Julia Peterson,Richard W Price,Deborah E Cabin,Robert L Nussbaum,Jörg Brühmann,Roland Brandt,Chadwick W Christine,Michael J Aminoff,Marc K Hellerstein

Gastroenterology 137:598-606, 606.e1-2 PubMed19272386

2009

Release of endogenous opioids from duodenal enteroendocrine cells requires Trpm5.

Applications

ICC/IF

Species

Mouse

Zaza Kokrashvili,Deniliz Rodriguez,Valeriya Yevshayeva,Hang Zhou,Robert F Margolskee,Bedrich Mosinger

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Chromogranin B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin B antibody (AB12242)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise