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AB249010

Anti-Chromogranin B antibody [EPR9567] - BSA and Azide free

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Rabbit Recombinant Monoclonal Chromogranin B antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

SCG1, CHGB, Secretogranin-1, Chromogranin-B, Secretogranin I, CgB, SgI

1 Images
Western blot - Anti-Chromogranin B antibody [EPR9567] - BSA and Azide free (AB249010)
  • WB

Unknown

Western blot - Anti-Chromogranin B antibody [EPR9567] - BSA and Azide free (AB249010)

This data was developed using ab151568, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Chromogranin B antibody [EPR9567] - BSA and Azide free (ab249010) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 78 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9567

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

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Primary Antibodies

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Primary Antibodies

AB249000

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Western blot - Anti-NCOA62/SNW1 antibody [EPR9988(B)] - BSA and Azide free (AB249000)

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Primary Antibodies

AB124916

Anti-SUN2 antibody [EPR6557]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUN2 antibody [EPR6557] (AB124916)

  • 5
  • 6
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Reactivity data

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Product details

ab249010 is the carrier-free version of ab151568.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Chromogranin B also known as secretogranin I is a member of the granin protein family and is sometimes referred to alongside chromogranin A and other chromogranins. Chromogranin B is a secretory protein with a known mass of approximately 80 kDa. This protein expresses primarily in neuroendocrine cells where it localizes to the secretory granules. Chromogranins including chromogranin B play a role in the formation and stabilization of secretory granules important for storing and releasing peptide hormones and neuropeptides.
Biological function summary

Chromogranin B functions beyond merely being a constituent of secretory granules. It plays a role in the regulation of neuroendocrine secretion processes and interacts with the chromogranin core complex. This complex is involved in the packaging and sorting of peptides and hormones within cells. Chromogranin B also exhibits roles in modulating calcium-binding dynamics which is critical for cellular signaling and hormone secretion. Unlike chromogranin A chromogranin B is less abundant but still significant for cellular processes.

Pathways

Chromogranin B prominently aligns with the secretory pathway associated with neuroendocrine tissues. This pathway involves several proteins like chromogranin A that often work alongside chromogranin B to influence hormone release. Another pathway where chromogranin B plays a role involves the regulation of catecholamine release vital for stress responses. This particular involvement with catecholamines links chromogranin B to its role in neurotransmitter regulation.

Chromogranin B shows an association with conditions such as neuroendocrine tumors and hypertension. In neuroendocrine tumors chromogranin B can serve as a biomarker since its levels often elevate in these conditions akin to chromogranin A. Hypertension studies suggest connections between chromogranin B and the dysregulation of catecholamine release impacting blood pressure regulation. Understanding the role of chromogranin B and its related proteins can help in diagnosing and potentially targeting these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides.
See full target information CHGB
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com