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Rabbit Recombinant Monoclonal Chromogranin C/SGII antibody. Suitable for IHC-P, ICC/IF, WB, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.


Images

Western blot - Anti-Chromogranin C/SGII antibody [EPR23876-52] (AB275019), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody [EPR23876-52] (AB275019), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (AB275019), expandable thumbnail
  • Western blot - Anti-Chromogranin C/SGII antibody [EPR23876-52] (AB275019), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody [EPR23876-52] (AB275019), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPWBIHC-FrFlow Cyt (Intra)
Human
Tested
Expected
Not recommended
Tested
Expected
Expected
Mouse
Tested
Tested
Not recommended
Tested
Tested
Tested
Rat
Tested
Tested
Not recommended
Tested
Tested
Tested

Tested
Tested

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Human

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

-

Species

Rat

Dilution info

1/50

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Species

Rat

Dilution info

1/500

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

-

Species

Rat

Dilution info

1/500

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Neuroendocrine protein of the granin family that regulates the biogenesis of secretory granules.

Alternative names

Secretogranin-2, Chromogranin-C, Secretogranin II, SgII, SCG2, CHGC

Recommended products

Rabbit Recombinant Monoclonal Chromogranin C/SGII antibody. Suitable for IHC-P, ICC/IF, WB, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR23876-52

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Chromogranin C also known as secretogranin II (SGII) is a member of the granin family and weighs approximately 68 kDa. This protein is mainly located within the dense core secretory granules of endocrine and neuronal cells. It plays a mechanical role in the storage and release of peptide hormones and neurotransmitters. Chromogranin C is found in various regions of the brain adrenal medulla and in the pituitary gland reflecting its involvement in the neuroendocrine system.

Biological function summary

This protein functions in the regulation of peptide hormone secretion and is part of a complex with other granin proteins such as Chromogranin A. It modulates the granule's environment by affecting the pH and ionic composition which influences the storage and release of hormones and neurotransmitters. Chromogranin C participates in the production of biologically active peptides after proteolytic processing playing roles in the modulation of endocrine and neuronal activities.

Pathways

Chromogranin C connects to the neuroendocrine signaling pathways and regulated secretory pathway. Within these pathways it interacts with other proteins such as the prohormone convertases and furin which handle peptide precursors' cleavage into active hormones and neurotransmitters. This procession is important for maintaining cellular communication and response mechanisms linking Chromogranin C to cellular signaling and biochemical processes that control physiological homeostasis and stress responses.

Associated diseases and disorders

Chromogranin C correlates with neurodegenerative diseases like Alzheimer's disease and psychiatric disorders such as depression. These connections arise through its relationship with amyloid precursor protein in Alzheimer's and alterations in serotonergic systems in depression. The expression levels and processing of Chromogranin C may serve as a biomarker for these diseases highlighting its potential role in the pathogenesis and progression of neuro-psychiatric conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Western blot - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019), expandable thumbnail

    Western blot - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 26 seconds; Lane 2: 48 seconds.

    Chromogranin C/SGII is proteolytically processed to small fragments. 81-kDa, 22 to 48 kDa forms are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 2282535, 8492910, 16357044, 7883050).

    All lanes: Western blot - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019) at 1/1000 dilution

    Lane 1: Mouse hypothalamus tissue lysate at 40 µg

    Lane 2: Rat hypothalamus tissue lysate at 40 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 71 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)

    Immunofluorescent analysis of 100% methanol-fixed PC-12 cells labelling Chromogranin C/SGII with ab275019 at 1/50 (10.68 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in PC-12 cell line. is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)

    Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling Chromogranin C/SGII with ab275019 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining in pancreatic islet (PMID: 30690892). The section was incubated with ab275019 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Western blot - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019), expandable thumbnail

    Western blot - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 37 seconds; Lane 2: 15 seconds; Lane3-4: 5.5 seconds; Lane5: 1 second.

    Chromogranin C/SGII is proteolytically processed to small fragments. 81-kDa, 55-kDa, 22 to 48 kDa forms are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 2282535, 8492910, 16357044, 7883050).

    All lanes: Western blot - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019) at 1/1000 dilution

    Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 40 µg

    Lane 2: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 40 µg

    Lane 3: Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate at 40 µg

    Lane 4: GH3 (rat pituitary epithelial) whole cell lysate at 40 µg

    Lane 5: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 40 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 71 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Beta-TC-6 cells labelling Chromogranin C/SGII with ab275019 at 1/50 (10.68 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Beta-TC-6 cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)

    Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Chromogranin C/SGII with ab275019 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining in mouse pancreatic islet (PMID: 30690892). The section was incubated with ab275019 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat pancreas tissue labeling Chromogranin C/SGII with ab275019 at 1/500 (1.068 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat pancreatic islet is observed. The nuclear counterstain was DAPI (Blue). Insulin was counterstained using Anti-Insulin antibody [K36aC10] ab6995 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Red).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Flow Cytometry (Intracellular) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized PC-12 (Rat adrenal gland pheochromocytoma) cells labelling Chromogranin C/SGII with ab275019 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Frozen sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pancreas tissue labeling Chromogranin C/SGII with ab275019 at 1/500 (1.068 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse pancreatic islet is observed. Insulin was counterstained using Anti-Insulin antibody [K36aC10] ab6995 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Red).The nuclear counterstain was DAPI (Blue).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Flow Cytometry (Intracellular) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Beta-TC-6 (Mouse pancreas insulinoma beta cell) cells labelling Chromogranin C/SGII with ab275019 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Chromogranin C/SGII with ab275019 at 1/2000 (0.267 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining in human pancreatic islet (PMID: 30690892)The section was incubated with ab275019 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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