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AB224495

Anti-CHSS2 antibody

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(4 Publications)

Rabbit Polyclonal CHSS2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CHPF aa 250-400.

View Alternative Names

CSS2, UNQ651/PRO1281, CHPF, Chondroitin sulfate synthase 2, Chondroitin glucuronyltransferase 2, Chondroitin-polymerizing factor, Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II, N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase II, N-acetylgalactosaminyltransferase 2, ChPF

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-CHSS2 antibody (AB224495)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CHSS2 antibody (AB224495)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for CHSS2 (green) using ab224495 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHSS2 antibody (AB224495)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHSS2 antibody (AB224495)

Paraffin-embedded human small intestine tissue stained for CHSS2 using ab224495 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-CHSS2 antibody (AB224495)
  • WB

Supplier Data

Western blot - Anti-CHSS2 antibody (AB224495)

All lanes:

Western blot - Anti-CHSS2 antibody (ab224495)

Lane 1:

Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 2:

CHSS2 overepression HEK-293T cell lysate (Co-expressed with a C-terminal myc-DDK tag, ~3.1 kDa)

Predicted band size: 85 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human CHPF aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

Q8IZ52

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Seems to act as a specific activating factor for CHSY1 in chondroitin polymerization (PubMed : 12716890).. Isoform 2. May facilitate PRKN transport into the mitochondria. In collaboration with PRKN, may enhance cell viability and protect cells from oxidative stress.
See full target information CHPF
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Renal failure 47:2553384 PubMed40948471

2025

A novel membrane-addressing domain mutation () in Chinese twins with FSGS: implications for podocyte injury and ion channel disruption.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Peng,Dan Chang,Tingyu Chen,Sipei Chen,Li Wang,Guisen Li

Frontiers in oncology 12:856712 PubMed35372047

2022

Bioinformatics and Experimental Analysis of the Prognostic and Predictive Value of the CHPF Gene on Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wan-Wan Li,Bin Liu,Shu-Qing Dong,Shi-Qing He,Yu-Ying Liu,Si-Yu Wei,Jing-Yi Mou,Jia-Xin Zhang,Zhao Liu

Cell cycle (Georgetown, Tex.) 20:591-602 PubMed33651657

2021

Identification of chondroitin polymerizing factor (CHPF) as tumor promotor in cholangiocarcinoma through regulating cell proliferation, cell apoptosis and cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Duan,Jianhui Yang,Bo Jiang,Wenbin Duan,Rongguang Wei,Hui Zhang,Xianhai Mao

Biochemistry and cell biology = Biochimie et biolo 98:362-369 PubMed32383983

2020

Chondroitin polymerizing factor (CHPF) contributes to malignant proliferation and migration of hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qigang Xu,Wei Lin,Chonglin Tao,Xiaming Huang,Junjian Li
View all publications
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Product promise

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