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AB75052

Anti-CHSS2 antibody

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(2 Publications)

Rabbit Polyclonal CHSS2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CHPF.

View Alternative Names

CSS2, UNQ651/PRO1281, CHPF, Chondroitin sulfate synthase 2, Chondroitin glucuronyltransferase 2, Chondroitin-polymerizing factor, Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II, N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase II, N-acetylgalactosaminyltransferase 2, ChPF

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-CHSS2 antibody (AB75052)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CHSS2 antibody (AB75052)

Immunofluorescence analysis of CHSS2 in MCF-7 cells, using ab75052 at a 1/500 dilution.
Left image : untreated.
Right image : treated with immunizing peptide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHSS2 antibody (AB75052)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHSS2 antibody (AB75052)

Immunohistochemical analysis of CHSS2 in paraffin embedded human cervix carcinoma tissue, using ab75052 at a 1/50 dilution.
Left image : untreated.
Right image : treated with immunizing peptide.

Western blot - Anti-CHSS2 antibody (AB75052)
  • WB

Unknown

Western blot - Anti-CHSS2 antibody (AB75052)

All lanes:

Western blot - Anti-CHSS2 antibody (ab75052) at 1/500 dilution

Lane 1:

Extracts from LOVO cells at 5 µg

Lane 2:

Extracts from K562 cells at 5 µg

Lane 3:

Extracts from LOVO cells plus 5µg immunizing peptide at 5 µg

Predicted band size: 85 kDa

Observed band size: 85 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human CHPF. The exact immunogen used to generate this antibody is proprietary information.

Q8IZ52

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Seems to act as a specific activating factor for CHSY1 in chondroitin polymerization (PubMed : 12716890).. Isoform 2. May facilitate PRKN transport into the mitochondria. In collaboration with PRKN, may enhance cell viability and protect cells from oxidative stress.
See full target information CHPF
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of medical sciences 18:207-215 PubMed33390789

2021

Reduction of pl-CSA through ChSy-2 knockout inhibits tumorigenesis and metastasis of choriocarcinoma in JEG3 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Juzuo Zhang,Zhilong Chen,Baobei Wang,Jie Chen,Tianxia Xiao,Jian V Zhang,Shiling Chen,Xiujun Fan

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:16581-16600 PubMed33089528

2020

Chondroitin synthase-3 regulates nucleus pulposus degeneration through actin-induced YAP signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Leixin Wei,Peng Cao,Chen Xu,Huajian Zhong,Xiukun Wang,Meizhu Bai,Bo Hu,Ruizhe Wang,Ning Liu,Ye Tian,Huajiang Chen,Jinsong Li,Wen Yuan
View all publications
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Product promise

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