AB16141

Anti-CHX10 antibody

Name: Anti-CHX10 antibody. Sheep polyclonal (ab16141) | Abcam
Brand: Abcam Limited
SKU: AB16141
Price: 595 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(33 Publications )

Sheep Polyclonal CHX10 antibody. Suitable for WB and reacts with Mouse, Rat samples. Cited in 33 publications. Immunogen corresponding to Recombinant Fragment Protein within Human VSX2 aa 250 to C-terminus.

View Alternative Names

CHX10, HOX10, VSX2, Visual system homeobox 2, Ceh-10 homeodomain-containing homolog, Homeobox protein CHX10

1 Images

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Western blot - Anti-CHX10 antibody (AB16141)

Western blot analysis using ab16141 at 1 μg/ml on rat liver (A), rat retina tissue lysate (B), mouse liver (C) and mouse retina tissue lysate (D).

All lanes:

Western blot - Anti-CHX10 antibody (ab16141)

Predicted band size: 39 kDa

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Key facts

Host species

Sheep

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

applications

Immunogen

Recombinant Fragment Protein within Human VSX2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P58304

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

Chx10 is a 46kDa homeodomain protein of the paired-like class that is essential for development of the mammalian eye. Mutations in Chx10 cause microphthalmia, a cause of congenital blindness in humans, and the ocular retardation (or) phenotype in mice. In the developing mouse retina Chx10 is expressed in retinal progenitors, while in the mature retina, Chx10 expression becomes restricted to bipolar neurons. Concurrent with these expression patterns, the Chx10-/- (or) retina is thin due to a defect in proliferation of retinal progenitors, and lacks bipolar neurons. Chx10 is also expressed in the developing brainstem, thalamus, and spinal cord.

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Properties and storage information

Form

Liquid

Purification technique

Precipitation Ammonium Sulphate

Storage buffer

Preservative: 0.08% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a transcriptional regulator through binding to DNA at the consensus sequence 5'-[TC]TAATT[AG][AG]-3' upstream of gene promoters (PubMed : 27301076). Plays a significant role in the specification and morphogenesis of the sensory retina (By similarity). May play a role in specification of V2a interneurons during spinal cord development (By similarity). Mediates differentiation of V2a interneurons by repression of motor neuron gene transcription, via competitively binding to response elements that are activated by the ISL1-LHX3 complex, such as VSX1 (PubMed : 17919464, PubMed : 27477290). Acts as a positive transcriptional regulator of NXNL1; regulation is significantly increased in synergy with VSX1 (By similarity). Acts as a negative transcriptional regulator of MITF (By similarity). Represses SAG transcription by competitive inhibition of ISL1-LHX3 response elements (PubMed : 16236706, PubMed : 27477290). Binds to the photoreceptor conserved element-1 (PCE-1) in the promoter of rod photoreceptor arrestin SAG and acts as a transcriptional repressor (By similarity). Involved in the development of retinal ganglion cells (RGCs) which leads to release of SHH by RGCs, promoting Hedgehog signaling and subsequent proliferation of retinal progenitor cells (By similarity). Participates in the development of the cells of the inner nuclear layer, by promoting postnatal differentiation of bipolar cells with a comparable inhibition of rod cell differentiation (By similarity). May play a role in the maintenance of neural retina identity during development by regulation of canonical Wnt genes and CTNNB1 localization, suggesting a role in the regulation of canonical Wnt signaling (PubMed : 27301076).

See full target information VSX2

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Publications (33)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eads4938 PubMed40749050

2025

Precise rewiring of corticospinal axons and spinal interneurons via near-infrared optogenetics for spinal cord injury treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Ji,Jun Yan,Chenxi Li,Yuhui Sun,Shoupeng Wang,Jin Tao,Jian Liu,Yaobo Liu

Journal of neuroscience research 102:e70007 PubMed39704330

2024

Engrailed1 in Parvalbumin-Positive Neurons Regulates Eye-Specific Retinogeniculate Segregation and Visual Function.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqing Chen,Chengyong Jiang,Biao Yan,Jiayi Zhang

International journal of medical sciences 21:1472-1490 PubMed38903914

2024

Retinal ganglion cell type-specific expression of synuclein family members revealed by scRNA-sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Qingwen Yang,Lin Liu,Fang He,Wenna Zhao,Zhongqun Chen,Xiaotian Wu,Bilin Rao,Xin Lin,Fangyuan Mao,Jia Qu,Jun Zhang

International journal of molecular sciences 25: PubMed38338722

2024

The Analysis of Embryoid Body Formation and Its Role in Retinal Organoid Development.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Heredero Berzal,Ellie L Wagstaff,Anneloor L M A Ten Asbroek,Jacoline B Ten Brink,Arthur A Bergen,Camiel J F Boon

iScience 26:107887 PubMed37771660

2023

Acquisition of neural fate by combination of BMP blockade and chromatin modification.

Applications

Unspecified application

Species

Unspecified reactive species

Agnes Lee Chen Ong,Toshiya Kokaji,Arisa Kishi,Yoshihiro Takihara,Takuma Shinozuka,Ren Shimamoto,Ayako Isotani,Manabu Shirai,Noriaki Sasai

eLife 12: PubMed36975211

2023

Reserpine maintains photoreceptor survival in retinal ciliopathy by resolving proteostasis imbalance and ciliogenesis defects.

Applications

Unspecified application

Species

Unspecified reactive species

Holly Y Chen,Manju Swaroop,Samantha Papal,Anupam K Mondal,Hyun Beom Song,Laura Campello,Gregory J Tawa,Florian Regent,Hiroko Shimada,Kunio Nagashima,Natalia de Val,Samuel G Jacobson,Wei Zheng,Anand Swaroop

BMC biology 20:205 PubMed36127701

2022

Activation of oxytocin receptors in mouse GABAergic amacrine cells modulates retinal dopaminergic signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Songhui Hu,Yurong Wang,Xu Han,Min Dai,Yongxing Zhang,Yuanyuan Ma,Shijun Weng,Lei Xiao

Science advances 8:eabm9027 PubMed35675393

2022

The role of ipRGCs in ocular growth and myopia development.

Applications

Unspecified application

Species

Unspecified reactive species

Ai-Lin Liu,Yun-Feng Liu,Ge Wang,Yu-Qi Shao,Chen-Xi Yu,Zhe Yang,Zi-Rui Zhou,Xu Han,Xue Gong,Kang-Wei Qian,Li-Qin Wang,Yuan-Yuan Ma,Yong-Mei Zhong,Shi-Jun Weng,Xiong-Li Yang

Neuroscience 491:75-86 PubMed35306143

2022

Rabies Anterograde Monosynaptic Tracing Allows Identification of Postsynaptic Circuits Receiving Distinct Somatosensory Input.

Applications

Unspecified application

Species

Unspecified reactive species

Sofia Pimpinella,Ilaria Sauve,Stephan Dietrich,Niccolò Zampieri

Journal of neuroinflammation 18:215 PubMed34544421

2021

The role of interferon regulatory factor 8 for retinal tissue homeostasis and development of choroidal neovascularisation.

Applications

Unspecified application

Species

Unspecified reactive species

Peipei Zhang,Anja Schlecht,Julian Wolf,Stefaniya Boneva,Yannik Laich,Jana Koch,Franziska Ludwig,Myriam Boeck,Adrian Thien,Carmen Härdtner,Katrin Kierdorf,Hansjürgen Agostini,Günther Schlunck,Marco Prinz,Ingo Hilgendorf,Peter Wieghofer,Clemens Lange

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CHX10 antibody

Western blot - Anti-CHX10 antibody (AB16141)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (33)

Product promise