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AB187164

Anti-Chymotrypsin antibody [EPR13859]

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(1 Publication)

Rabbit Recombinant Monoclonal Chymotrypsin antibody. Suitable for Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CLCR, CTRC, Chymotrypsin-C, Caldecrin

2 Images
Flow Cytometry (Intracellular) - Anti-Chymotrypsin antibody [EPR13859] (AB187164)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Chymotrypsin antibody [EPR13859] (AB187164)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Ycells labeling Chymotrypsinwith ab187164 at 1/270 dilution (red), compared to a Rabbit monclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibodyat 1/150 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chymotrypsin antibody [EPR13859] (AB187164)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chymotrypsin antibody [EPR13859] (AB187164)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Chymotrypsin with ab187164 at 1/2000 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Carrier free

    Anti-Chymotrypsin antibody [EPR13859] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13859

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/270", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000 - 1/4000", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Chymotrypsin also known as chymotrypsinogen when in its inactive precursor form and chymotrypsine stands out as a serine protease enzyme with a molecular weight around 25 kDa. Mechanically chymotrypsin catalyzes the hydrolysis of peptide bonds especially those containing aromatic amino acids like phenylalanine tyrosine and tryptophan. The enzyme exhibits a preference for cleaving at the carboxyl side of these amino acids. Chymotrypsin is mainly expressed in the acinar cells of the pancreas where it aids in the digestive process. The enzyme becomes active upon cleavage by trypsin another serine protease forming chymotrypsin and trypsin as active proteolytic enzymes.
Biological function summary

The enzyme plays an essential role in the digestion of proteins within the small intestine. Chymotrypsin contributes significantly to breaking down dietary proteins into smaller peptides and amino acids which are easier to absorb. During this process chymotrypsin partners with other pancreatic enzymes such as trypsin making up a proteolytic cascade. Chymotrypsin activity ensures efficient protein digestion which is important for nutrient absorption and metabolism. The enzyme's product which results from substrate cleavage is integral to various physiological processes.

Pathways

Chymotrypsin is intricately involved in the digestive enzymatic cascade that ensures proper digestion and absorption of nutrients. The proteolytic activity of chymotrypsin and trypsin in the digestive system forms part of the broader system of exocrine pancreatic secretion. This pathway which involves various enzymes like elastase and chemotrypsin facilitates the efficient processing of proteins and is critical for maintaining metabolic homeostasis. Chymotrypsin's interaction with other enzymes highlights its significance in these pathways.

Abnormal chymotrypsin activity can link to certain pancreatic disorders such as chronic pancreatitis where enzyme secretion disrupts. This disruption may relate through the dysfunction of other enzymes including trypsin. Moreover hereditary pancreatitis associates with mutations affecting the regulation of the enzyme or its precursor chymotrypsinogen. Understanding these associations helps to elucidate the role of chymotrypsin protein in various pathological states shedding light on the importance of maintaining its normal function to prevent disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates activation and degradation of trypsinogens and procarboxypeptidases by targeting specific cleavage sites within their zymogen precursors. Has chymotrypsin-type protease activity and hypocalcemic activity.
See full target information CTRC
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 18:4030-4039 PubMed31516605

2019

Genome-wide bioinformatics analysis reveals CTCFL is upregulated in high-grade epithelial ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mi Gong,Changsheng Yan,Yi Jiang,Huangyang Meng,Mingming Feng,Wenjun Cheng
View all publications
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Product promise

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