AB250920

Anti-Chymotrypsin antibody [EPR13859] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Chymotrypsin antibody. Carrier free. Suitable for Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CLCR, CTRC, Chymotrypsin-C, Caldecrin

2 Images

Flow Cytometry (Intracellular) - Anti-Chymotrypsin antibody [EPR13859] - BSA and Azide free (AB250920)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Chymotrypsin antibody [EPR13859] - BSA and Azide free (AB250920)

This data was developed using ab187164, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed SH-SY5Y cells labeling Chymotrypsin with ab187164 at 1/270 dilution (red), compared to a Rabbit monclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chymotrypsin antibody [EPR13859] - BSA and Azide free (AB250920)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chymotrypsin antibody [EPR13859] - BSA and Azide free (AB250920)

This data was developed using ab187164, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Chymotrypsin with ab187164 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13859

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Product details

ab250920 is the carrier-free version of ab187164.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Chymotrypsin also known as chymotrypsinogen when in its inactive precursor form and chymotrypsine stands out as a serine protease enzyme with a molecular weight around 25 kDa. Mechanically chymotrypsin catalyzes the hydrolysis of peptide bonds especially those containing aromatic amino acids like phenylalanine tyrosine and tryptophan. The enzyme exhibits a preference for cleaving at the carboxyl side of these amino acids. Chymotrypsin is mainly expressed in the acinar cells of the pancreas where it aids in the digestive process. The enzyme becomes active upon cleavage by trypsin another serine protease forming chymotrypsin and trypsin as active proteolytic enzymes.

Biological function summary

The enzyme plays an essential role in the digestion of proteins within the small intestine. Chymotrypsin contributes significantly to breaking down dietary proteins into smaller peptides and amino acids which are easier to absorb. During this process chymotrypsin partners with other pancreatic enzymes such as trypsin making up a proteolytic cascade. Chymotrypsin activity ensures efficient protein digestion which is important for nutrient absorption and metabolism. The enzyme's product which results from substrate cleavage is integral to various physiological processes.

Pathways

Chymotrypsin is intricately involved in the digestive enzymatic cascade that ensures proper digestion and absorption of nutrients. The proteolytic activity of chymotrypsin and trypsin in the digestive system forms part of the broader system of exocrine pancreatic secretion. This pathway which involves various enzymes like elastase and chemotrypsin facilitates the efficient processing of proteins and is critical for maintaining metabolic homeostasis. Chymotrypsin's interaction with other enzymes highlights its significance in these pathways.

Abnormal chymotrypsin activity can link to certain pancreatic disorders such as chronic pancreatitis where enzyme secretion disrupts. This disruption may relate through the dysfunction of other enzymes including trypsin. Moreover hereditary pancreatitis associates with mutations affecting the regulation of the enzyme or its precursor chymotrypsinogen. Understanding these associations helps to elucidate the role of chymotrypsin protein in various pathological states shedding light on the importance of maintaining its normal function to prevent disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulates activation and degradation of trypsinogens and procarboxypeptidases by targeting specific cleavage sites within their zymogen precursors. Has chymotrypsin-type protease activity and hypocalcemic activity.

See full target information CTRC

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:3728 PubMed37349339

2023

NBEAL2 deficiency in humans leads to low CTLA-4 expression in activated conventional T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Laure Delage,Francesco Carbone,Quentin Riller,Jean-Luc Zachayus,Erwan Kerbellec,Armelle Buzy,Marie-Claude Stolzenberg,Marine Luka,Camille de Cevins,Georges Kalouche,Rémi Favier,Alizée Michel,Sonia Meynier,Aurélien Corneau,Caroline Evrard,Nathalie Neveux,Sébastien Roudières,Brieuc P Pérot,Mathieu Fusaro,Christelle Lenoir,Olivier Pellé,Mélanie Parisot,Marc Bras,Sébastien Héritier,Guy Leverger,Anne-Sophie Korganow,Capucine Picard,Sylvain Latour,Bénédicte Collet,Alain Fischer,Bénédicte Neven,Aude Magérus,Mickaël Ménager,Benoit Pasquier,Frédéric Rieux-Laucat

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Chymotrypsin antibody [EPR13859] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-Chymotrypsin antibody [EPR13859] - BSA and Azide free (AB250920)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chymotrypsin antibody [EPR13859] - BSA and Azide free (AB250920)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise