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AB154525

Anti-cIAP1 antibody

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(3 Publications)

Rabbit Polyclonal cIAP1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BIRC2 aa 300-600.

View Alternative Names

API1, MIHB, RNF48, BIRC2, Baculoviral IAP repeat-containing protein 2, Cellular inhibitor of apoptosis 1, IAP homolog B, Inhibitor of apoptosis protein 2, RING finger protein 48, RING-type E3 ubiquitin transferase BIRC2, TNFR2-TRAF-signaling complex protein 2, C-IAP1, hIAP-2, hIAP2

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody (AB154525)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody (AB154525)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue stained for cIAP1 protein at cytoplasm on mouse kidney with ab154525 at a 1/500 dilution.

Antigen Retrieval : EDTA based, pH 8.0 buffer, 15min.

Western blot - Anti-cIAP1 antibody (AB154525)
  • WB

Supplier Data

Western blot - Anti-cIAP1 antibody (AB154525)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-cIAP1 antibody (ab154525) at 1/1000 dilution

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lane 2:

Transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 70 kDa

false

Western blot - Anti-cIAP1 antibody (AB154525)
  • WB

Supplier Data

Western blot - Anti-cIAP1 antibody (AB154525)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-cIAP1 antibody (ab154525) at 1/500 dilution

Lane 1:

Non-transfected 293T whole cell extracts at 50 µg

Lane 2:

Transfected 293T whole cell extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 70 kDa

false

Western blot - Anti-cIAP1 antibody (AB154525)
  • WB

Supplier Data

Western blot - Anti-cIAP1 antibody (AB154525)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-cIAP1 antibody (ab154525) at 1/1000 dilution

All lanes:

NCI-H929 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 70 kDa

false

Western blot - Anti-cIAP1 antibody (AB154525)
  • WB

Unknown

Western blot - Anti-cIAP1 antibody (AB154525)

7.5% SDS PAGE

All lanes:

Western blot - Anti-cIAP1 antibody (ab154525) at 1/1000 dilution

All lanes:

rat muscle lysate at 50 µg

Predicted band size: 70 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human, Mouse

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human BIRC2 aa 300-600. The exact immunogen used to generate this antibody is proprietary information.

Q13490

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The cellular inhibitor of apoptosis protein 1 (cIAP1) also referred to as BIRC2 is a protein with a molecular mass of approximately 70 kDa. cIAP1 belongs to the inhibitor of apoptosis (IAP) family and contains several distinct domains important for its function including three Baculoviral IAP Repeat (BIR) domains a ubiquitin-associated (UBA) domain and a really interesting new gene (RING) domain. This protein is expressed in various tissues but predominantly in cells engaged in apoptosis regulation such as immune cells.
Biological function summary

CIAP1 plays an important role in regulating apoptosis by inhibiting the activity of certain caspases including caspase-3 and caspase-7. It is often part of larger protein complexes that include other members of the IAP family such as cIAP2. By binding directly to tumor necrosis factor receptor-associated factors (TRAFs) cIAP1 helps modulate signaling pathways that determine cell survival or death contributing to cellular homeostasis.

Pathways

CIAP1 is intricately involved in the NF-κB signaling pathway and the TNF receptor signaling pathway. Its interaction with TRAFs links it to these critical pathways helping to control inflammatory and immune responses. Other proteins related to cIAP1 through these pathways include RIP1 and TRAF2 both important for transmitting signals that affect cell fate decisions.

CIAP1 has significant implications in cancer and autoimmune disorders. In many cancers overexpression of cIAP1 contributes to uncontrolled cell proliferation by preventing apoptosis therefore aiding tumor survival and progression. Moreover its dysregulation is linked to disorders such as rheumatoid arthritis where improper cell death regulation leads to tissue damage. cIAP1 interacts with and regulates the activity of the protein XIAP in contributing to these diseases making it a potential target for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling, and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions : acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin-protein ligase activity include : RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF2, DIABLO/SMAC, MAP3K14/NIK, MAP3K5/ASK1, IKBKG/NEMO, IKBKE and MXD1/MAD1. Can also function as an E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Can stimulate the transcriptional activity of E2F1. Plays a role in the modulation of the cell cycle.
See full target information BIRC2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:3499 PubMed40221432

2025

Fibromodulin selectively accelerates myofibroblast apoptosis in cutaneous wounds by enhancing interleukin 1β signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Wenlu Jiang,Xiaoxiao Pang,Pin Ha,Chenshuang Li,Grace Xinlian Chang,Yuxin Zhang,Lawrence A Bossong,Kang Ting,Chia Soo,Zhong Zheng

Journal of cellular and molecular medicine : PubMed33939305

2021

cIAP1/2 are involved in the radiosensitizing effect of birinapant on NSCLC cell line in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Sun,Yanan Du,Ming Yao,Qin Wang,Kaihua Ji,Liqing Du,Chang Xu,Ningning He,Jinhan Wang,Manman Zhang,Yang Liu,Yan Wang,Kaixue Wen,Qiang Liu

Journal of cancer prevention 24:155-162 PubMed31624721

2019

Involvement of Up-regulation of Death Receptors and Bim in Hispolon-mediated TNF-related Apoptosis-inducing Ligand Sensitization in Human Renal Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Mi Yun,Kyoung-Jin Min,Taeg Kyu Kwon
View all publications
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