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Rabbit Recombinant Monoclonal cIAP1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 26 publications.

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Images

Western blot - Anti-cIAP1 antibody [EPR4673] (AB108361), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody [EPR4673] (AB108361), expandable thumbnail
  • Western blot - Anti-cIAP1 antibody [EPR4673] (AB108361), expandable thumbnail
  • Western blot - Anti-cIAP1 antibody [EPR4673] (AB108361), expandable thumbnail
  • Western blot - Anti-cIAP1 antibody [EPR4673] (AB108361), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/500
Notes

For purification use at 1/50 - 1/100

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling, and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF2, DIABLO/SMAC, MAP3K14/NIK, MAP3K5/ASK1, IKBKG/NEMO, IKBKE and MXD1/MAD1. Can also function as an E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Can stimulate the transcriptional activity of E2F1. Plays a role in the modulation of the cell cycle.

Alternative names

API1, MIHB, RNF48, BIRC2, Baculoviral IAP repeat-containing protein 2, Cellular inhibitor of apoptosis 1, IAP homolog B, Inhibitor of apoptosis protein 2, RING finger protein 48, RING-type E3 ubiquitin transferase BIRC2, TNFR2-TRAF-signaling complex protein 2, C-IAP1, hIAP-2, hIAP2

Recommended products

Rabbit Recombinant Monoclonal cIAP1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 26 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4673
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The cellular inhibitor of apoptosis protein 1 (cIAP1) also referred to as BIRC2 is a protein with a molecular mass of approximately 70 kDa. cIAP1 belongs to the inhibitor of apoptosis (IAP) family and contains several distinct domains important for its function including three Baculoviral IAP Repeat (BIR) domains a ubiquitin-associated (UBA) domain and a really interesting new gene (RING) domain. This protein is expressed in various tissues but predominantly in cells engaged in apoptosis regulation such as immune cells.

Biological function summary

CIAP1 plays an important role in regulating apoptosis by inhibiting the activity of certain caspases including caspase-3 and caspase-7. It is often part of larger protein complexes that include other members of the IAP family such as cIAP2. By binding directly to tumor necrosis factor receptor-associated factors (TRAFs) cIAP1 helps modulate signaling pathways that determine cell survival or death contributing to cellular homeostasis.

Pathways

CIAP1 is intricately involved in the NF-κB signaling pathway and the TNF receptor signaling pathway. Its interaction with TRAFs links it to these critical pathways helping to control inflammatory and immune responses. Other proteins related to cIAP1 through these pathways include RIP1 and TRAF2 both important for transmitting signals that affect cell fate decisions.

Associated diseases and disorders

CIAP1 has significant implications in cancer and autoimmune disorders. In many cancers overexpression of cIAP1 contributes to uncontrolled cell proliferation by preventing apoptosis therefore aiding tumor survival and progression. Moreover its dysregulation is linked to disorders such as rheumatoid arthritis where improper cell death regulation leads to tissue damage. cIAP1 interacts with and regulates the activity of the protein XIAP in contributing to these diseases making it a potential target for therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361), expandable thumbnail

    Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)

    Lanes 1- 2: Merged signal (red and green). Green - ab108361 observed at 70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab108361 was shown to react with cIAP1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human BIRC2 (cIAP1) knockout HeLa cell line ab265896 (knockout cell lysate Human BIRC2 (cIAP1) knockout HeLa cell lysate ab257372) was used. Wild-type HeLa and BIRC2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab108361 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: BIRC2 knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human BIRC2 (cIAP1) knockout HeLa cell line (Human BIRC2 (cIAP1) knockout HeLa cell line ab265896)

    Performed under reducing conditions.

    Predicted band size: 70 kDa

    Observed band size: 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody [EPR4673] (ab108361), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody [EPR4673] (ab108361)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling cIAP1 with Purified ab108361 at 1:500 dilution (2.82 µg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361), expandable thumbnail

    Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: cIAP1 knockout HAP1 cell lysate (20 μg)
    Lane 3: HeLa cell lysate (20 μg)
    Lane 4: Jurkat cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab108361 observed at 70 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab108361 was shown to specifically react with cIAP1 when cIAP1 knockout samples were used. Wild-type and cIAP1 knockout samples were subjected to SDS-PAGE. ab108361 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)

    Predicted band size: 70 kDa

  • Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361), expandable thumbnail

    Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)

    All lanes: Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361) at 1/2000 dilution

    All lanes: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 1/15 dilution

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/200000 dilution

    Predicted band size: 70 kDa

  • Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361), expandable thumbnail

    Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361)

    All lanes: Western blot - Anti-cIAP1 antibody [EPR4673] (ab108361) at 1/1000 dilution

    Lane 1: Jurkat cell lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Lane 3: HT29 cell lysate at 10 µg

    Lane 4: HepG2 cell lysate at 10 µg

    Predicted band size: 70 kDa

    Observed band size: 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody [EPR4673] (ab108361), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP1 antibody [EPR4673] (ab108361)

    ab108361, at 1/50, staining cIAP1 in paraffin-embedded Human spleen tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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