Rabbit Polyclonal cIAP2 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Baculoviral IAP repeat-containing protein 3 aa 300-550.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | WB | |
---|---|---|
Human | Tested | Tested |
Rat | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes or Tris-EDTA buffer (pH8.0) Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
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Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, IKBKE, TRAF1, and BCL10. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8.
API2, MIHC, RNF49, RNF49, MIHC, API2, BIRC3, Baculoviral IAP repeat-containing protein 3, Apoptosis inhibitor 2, Cellular inhibitor of apoptosis 2, IAP homolog C, Inhibitor of apoptosis protein 1, RING finger protein 49, RING-type E3 ubiquitin transferase BIRC3, TNFR2-TRAF-signaling complex protein 1, API2, C-IAP2, hIAP-1, hIAP1
Rabbit Polyclonal cIAP2 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Baculoviral IAP repeat-containing protein 3 aa 300-550.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
CIAP2 also called E3 ubiquitin-protein ligase and encoded by the BIRC3 gene functions to inhibit apoptosis. This protein belongs to the inhibitors of apoptosis (IAP) family and is around 69 kDa in mass. cIAP2 is widely expressed in various tissues including the epithelial and lymphoid cells. It operates mechanically by ubiquitinating substrates marking them for proteasomal degradation which is key in preventing cell death and maintaining cellular homeostasis.
CIAP2 serves as a regulator in cell survival by functioning within signaling cascades. It participates in the formation of the TNF receptor complex which influences the NF-κB signaling pathway and inflammation response. By interacting with several proteins cIAP2 modulates downstream signaling effects that can influence immune responses and cellular proliferation helping cells adapt and survive stressful conditions.
CIAP2 plays roles in both the NF-κB and the MAPK signaling pathways. Within these pathways cIAP2 interacts closely with proteins such as TRAF2 and RIP1. In the NF-κB pathway it helps in the regulation of inflammatory responses while in the MAPK pathway it contributes to cell growth and differentiation processes. These pathways highlight its function in regulating immune system responses and cell adaptability.
CIAP2 maintains connections with cancer and chronic inflammation. Abnormal expression or mutations of cIAP2 can lead to tumorigenesis due to disrupted apoptotic control linking it closely to cancers like lymphomas and leukemias. Additionally cIAP2 influences inflammatory diseases like Crohn's disease through its interactions with proteins such as NOD2 which further impacts the disease pathology. Proper regulation of cIAP2 is essential in avoiding unwanted cell survival and excessive inflammation highlighting its significance in various pathological conditions.
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Immunohistochemical analysis of paraffin-embedded Human SW480 xenograft tissue labelling cIAP2 with ab137393 at 1/100 dilution.
All lanes: Western blot - Anti-cIAP2 antibody (ab137393) at 1/1000 dilution
Lane 1: U-87 MG whole cell extracts at 30 µg
Lane 2: SK-N-SH whole cell extracts at 30 µg
Lane 3: IMR32 whole cell extracts at 30 µg
Lane 4: SK-N-AS whole cell extracts at 30 µg
Predicted band size: 68 kDa
7.5% SDS-PAGE.
All lanes: Western blot - Anti-cIAP2 antibody (ab137393) at 1/500 dilution
Lane 1: HeLa whole cell extract at 30 µg
Lane 2: HeLa cytosol extract at 30 µg
Lane 3: HeLa nuclear extract at 30 µg
Predicted band size: 68 kDa
7.5% SDS-PAGE.
All lanes: Western blot - Anti-cIAP2 antibody (ab137393) at 1/500 dilution
All lanes: Rat spleen lysate at 50 µg
Predicted band size: 68 kDa
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