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Rabbit Polyclonal cIAP2 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Baculoviral IAP repeat-containing protein 3 aa 300-550.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP2 antibody (AB137393), expandable thumbnail
  • Western blot - Anti-cIAP2 antibody (AB137393), expandable thumbnail
  • Western blot - Anti-cIAP2 antibody (AB137393), expandable thumbnail
  • Western blot - Anti-cIAP2 antibody (AB137393), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Baculoviral IAP repeat-containing protein 3 aa 300-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q13489

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Rat
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

or Tris-EDTA buffer (pH8.0)

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, IKBKE, TRAF1, and BCL10. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8.

Alternative names

Recommended products

Rabbit Polyclonal cIAP2 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Baculoviral IAP repeat-containing protein 3 aa 300-550.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Baculoviral IAP repeat-containing protein 3 aa 300-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q13489
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CIAP2 also called E3 ubiquitin-protein ligase and encoded by the BIRC3 gene functions to inhibit apoptosis. This protein belongs to the inhibitors of apoptosis (IAP) family and is around 69 kDa in mass. cIAP2 is widely expressed in various tissues including the epithelial and lymphoid cells. It operates mechanically by ubiquitinating substrates marking them for proteasomal degradation which is key in preventing cell death and maintaining cellular homeostasis.

Biological function summary

CIAP2 serves as a regulator in cell survival by functioning within signaling cascades. It participates in the formation of the TNF receptor complex which influences the NF-κB signaling pathway and inflammation response. By interacting with several proteins cIAP2 modulates downstream signaling effects that can influence immune responses and cellular proliferation helping cells adapt and survive stressful conditions.

Pathways

CIAP2 plays roles in both the NF-κB and the MAPK signaling pathways. Within these pathways cIAP2 interacts closely with proteins such as TRAF2 and RIP1. In the NF-κB pathway it helps in the regulation of inflammatory responses while in the MAPK pathway it contributes to cell growth and differentiation processes. These pathways highlight its function in regulating immune system responses and cell adaptability.

Associated diseases and disorders

CIAP2 maintains connections with cancer and chronic inflammation. Abnormal expression or mutations of cIAP2 can lead to tumorigenesis due to disrupted apoptotic control linking it closely to cancers like lymphomas and leukemias. Additionally cIAP2 influences inflammatory diseases like Crohn's disease through its interactions with proteins such as NOD2 which further impacts the disease pathology. Proper regulation of cIAP2 is essential in avoiding unwanted cell survival and excessive inflammation highlighting its significance in various pathological conditions.

Product promise

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4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP2 antibody (ab137393), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cIAP2 antibody (ab137393)

    Immunohistochemical analysis of paraffin-embedded Human SW480 xenograft tissue labelling cIAP2 with ab137393 at 1/100 dilution.

  • Western blot - Anti-cIAP2 antibody (ab137393), expandable thumbnail

    Western blot - Anti-cIAP2 antibody (ab137393)

    All lanes: Western blot - Anti-cIAP2 antibody (ab137393) at 1/1000 dilution

    Lane 1: U-87 MG whole cell extracts at 30 µg

    Lane 2: SK-N-SH whole cell extracts at 30 µg

    Lane 3: IMR32 whole cell extracts at 30 µg

    Lane 4: SK-N-AS whole cell extracts at 30 µg

    Predicted band size: 68 kDa

  • Western blot - Anti-cIAP2 antibody (ab137393), expandable thumbnail

    Western blot - Anti-cIAP2 antibody (ab137393)

    7.5% SDS-PAGE.

    All lanes: Western blot - Anti-cIAP2 antibody (ab137393) at 1/500 dilution

    Lane 1: HeLa whole cell extract at 30 µg

    Lane 2: HeLa cytosol extract at 30 µg

    Lane 3: HeLa nuclear extract at 30 µg

    Predicted band size: 68 kDa

  • Western blot - Anti-cIAP2 antibody (ab137393), expandable thumbnail

    Western blot - Anti-cIAP2 antibody (ab137393)

    7.5% SDS-PAGE.

    All lanes: Western blot - Anti-cIAP2 antibody (ab137393) at 1/500 dilution

    All lanes: Rat spleen lysate at 50 µg

    Predicted band size: 68 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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