AB10887

Anti-CID antibody

(2 Reviews)

(14 Publications)

Rabbit Polyclonal CID antibody. Suitable for WB, ICC/IF and reacts with Drosophila melanogaster samples. Cited in 14 publications.

View Alternative Names

CENP-A, CG13329, cid, Histone H3-like centromeric protein cid, CENP-A homolog, Centromere identifier protein

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-CID antibody (AB10887)

ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-CID antibody (AB10887)

Drosophila S2 cells treated with ab10887 at 1 : 100 dilution and fixed with 3% PFA for 3 mins. Red = DAPI Green = ab10887

This image is courtesy of Elaine Dunleavy

Project

Western blot - Anti-CID antibody (AB10887)

All lanes:

Western blot - Anti-CID antibody (ab10887) at 1 µg/mL

All lanes:

Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) (ab27258) at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution

Predicted band size: 26 kDa

Observed band size: 12 kDa,26 kDa,42 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Drosophila melanogaster

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Drosophila melanogaster": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore (PubMed : 11483958, PubMed : 16839185). Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation (PubMed : 11483958, PubMed : 16839185, PubMed : 24703848). May serve as an epigenetic mark that propagates centromere identity through replication and cell division (PubMed : 11483958, PubMed : 16839185).

See full target information cid

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 223: PubMed38180477

2024

Multimerization of a disordered kinetochore protein promotes accurate chromosome segregation by localizing a core dynein module.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica M McGory,Vikash Verma,Dylan M Barcelos,Thomas J Maresca

Cells 11: PubMed35883570

2022

Genetic Control of Kinetochore-Driven Microtubule Growth in Mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Julia V Popova,Gera A Pavlova,Alyona V Razuvaeva,Lyubov A Yarinich,Evgeniya N Andreyeva,Alina F Anders,Yuliya A Galimova,Fioranna Renda,Maria Patrizia Somma,Alexey V Pindyurin,Maurizio Gatti

Nature communications 11:2919 PubMed32522980

2020

Spt6 is a maintenance factor for centromeric CENP-A.

Applications

Unspecified application

Species

Unspecified reactive species

Georg O M Bobkov,Anming Huang,Sebastiaan J W van den Berg,Sreyoshi Mitra,Eduard Anselm,Vasiliki Lazou,Sarah Schunter,Regina Feederle,Axel Imhof,Alexandra Lusser,Lars E T Jansen,Patrick Heun

Nucleic acids research 47:10754-10770 PubMed31535131

2019

Phosphorylation of Drosophila CENP-A on serine 20 regulates protein turn-over and centromere-specific loading.

Applications

Unspecified application

Species

Unspecified reactive species

Anming Huang,Leopold Kremser,Fabian Schuler,Doris Wilflingseder,Herbert Lindner,Stephan Geley,Alexandra Lusser

PLoS genetics 15:e1008371 PubMed31527906

2019

RNAi-mediated depletion of the NSL complex subunits leads to abnormal chromosome segregation and defective centrosome duplication in Drosophila mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Gera A Pavlova,Julia V Popova,Evgeniya N Andreyeva,Lyubov A Yarinich,Mikhail O Lebedev,Alyona V Razuvaeva,Tatiana D Dubatolova,Anastasiya L Oshchepkova,Claudia Pellacani,Maria Patrizia Somma,Alexey V Pindyurin,Maurizio Gatti

BMC biology 16:68 PubMed29907103

2018

Ultrastructural analysis of mitotic Drosophila S2 cells identifies distinctive microtubule and intracellular membrane behaviors.

Applications

Unspecified application

Species

Unspecified reactive species

Anton Strunov,Lidiya V Boldyreva,Evgeniya N Andreyeva,Gera A Pavlova,Julia V Popova,Alena V Razuvaeva,Alina F Anders,Fioranna Renda,Alexey V Pindyurin,Maurizio Gatti,Elena Kiseleva

PLoS genetics 9:e1003719 PubMed24086141

2013

Histone chaperone NAP1 mediates sister chromatid resolution by counteracting protein phosphatase 2A.

Applications

Unspecified application

Species

Unspecified reactive species

Yuri M Moshkin,Cecile M Doyen,Tsung-Wai Kan,Gillian E Chalkley,Karen Sap,Karel Bezstarosti,Jeroen A Demmers,Zeliha Ozgur,Wilfred F J van Ijcken,C Peter Verrijzer

Current biology : CB 20:2090-9 PubMed21093267

2010

Specialization of a Drosophila capping protein essential for the protection of sperm telomeres.

Applications

IHC

Species

Drosophila melanogaster

Raphaëlle Dubruille,Guillermo A Orsi,Lætitia Delabaere,Elisabeth Cortier,Pierre Couble,Gabriel A B Marais,Benjamin Loppin

PloS one 5:e10120 PubMed20396651

2010

CenH3/CID incorporation is not dependent on the chromatin assembly factor CHD1 in Drosophila.

Applications

WB, ICC/IF

Species

Drosophila melanogaster, Drosophila melanogaster

Valerie Podhraski,Beatriz Campo-Fernandez,Hildegard Wörle,Paolo Piatti,Harald Niederegger,Günther Böck,Dmitry V Fyodorov,Alexandra Lusser

The Journal of cell biology 188:335-49 PubMed20142422

2010

SOLO: a meiotic protein required for centromere cohesion, coorientation, and SMC1 localization in Drosophila melanogaster.

Applications

ICC/IF

Species

Drosophila melanogaster

Rihui Yan,Sharon E Thomas,Jui-He Tsai,Yukihiro Yamada,Bruce D McKee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CID antibody

Immunocytochemistry/ Immunofluorescence - Anti-CID antibody (AB10887)

Western blot - Anti-CID antibody (AB10887)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (14)

Product promise