AB137395

Anti-CIKS/ACT1 antibody

Name: Anti-CIKS/ACT1 antibody (ab137395) | Abcam
Brand: Abcam Limited
SKU: AB137395

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(5 Publications)

Rabbit Polyclonal CIKS/ACT1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRAF3IP2 aa 350 to C-terminus.

View Alternative Names

C6orf2, C6orf4, C6orf5, C6orf6, TRAF3IP2, E3 ubiquitin ligase TRAF3IP2, Adapter protein CIKS, Connection to IKK and SAPK/JNK, E3 ubiquitin-protein ligase CIKS, Nuclear factor NF-kappa-B activator 1, TRAF3-interacting protein 2, ACT1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIKS/ACT1 antibody (AB137395)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIKS/ACT1 antibody (AB137395)

Immunohistochemical analysis of paraffin embedded Colon ca tissue labelled with ab137395 at 1/250 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-CIKS/ACT1 antibody (AB137395)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CIKS/ACT1 antibody (AB137395)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelled with ab137395 at 1/200 dilution.

Unknown

Western blot - Anti-CIKS/ACT1 antibody (AB137395)

7.5% SDS PAGE

All lanes:

Western blot - Anti-CIKS/ACT1 antibody (ab137395) at 1/1000 dilution

All lanes:

Hep G2 whole cell lysate at 30 µg

Predicted band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human TRAF3IP2 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O43734

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin ligase that catalyzes 'Lys-63'-linked polyubiquitination of target protein, enhancing protein-protein interaction and cell signaling (PubMed : 19825828). Transfers ubiquitin from E2 ubiquitin-conjugating enzyme UBE2V1-UBE2N to substrate protein (PubMed : 19825828). Essential adapter molecule in IL17A-mediated signaling (PubMed : 19825828, PubMed : 24120361). Upon IL17A stimulation, interacts with IL17RA and IL17RC receptor chains through SEFIR domains and catalyzes 'Lys-63'-linked polyubiquitination of TRAF6, leading to TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways (PubMed : 19825828).

See full target information TRAF3IP2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Inflammation : PubMed40257651

2025

PTGS2 Silencing Inhibits Ferroptosis in Staphylococcus Aureus-induced Osteomyelitis By Blocking the IL-17A Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Si-Rui Zhou,Wen-Guang Li,Li-Dan Yang,Hao Xiang,Ying Jin,Jian-Bo Feng,Hua-Zhang Xiong,Jiachen Peng

Respiratory research 25:429 PubMed39643876

2024

Silencing SMAD4 inhibits inflammation and ferroptosis in asthma by blocking the IL-17A signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xingyu Rao,Hong Luo,Kaiyuan Luo,Chaohua Hu

Journal of inflammation research 17:7221-7234 PubMed39416266

2024

SPHK2 Knockdown Inhibits the Proliferation and Migration of Fibroblast-Like Synoviocytes Through the IL-17 Signaling Pathway in Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxuan Zheng,Xiaolong Xiong,Ke Li,Guangji Wang,Huiyuan Cao,Hui Huang

Journal of inflammation research 17:5235-5249 PubMed39131209

2024

Xuanbi Yuyang Decoction Ameliorates DSS-Induced Colitis by Inhibiting Pyroptosis via Blocking of IL-17 Pathway Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Huang,Liqun Li,Chaowei Zheng,Jianfeng Li,Guangwen Chen,Yalu Chen

Molecular cancer therapeutics 20:296-306 PubMed33323457

2020

Birinapant Enhances Gemcitabine's Antitumor Efficacy in Triple-Negative Breast Cancer by Inducing Intrinsic Pathway-Dependent Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuemei Xie,Jangsoon Lee,Huey Liu,Troy Pearson,Alexander Y Lu,Debu Tripathy,Gayathri R Devi,Chandra Bartholomeusz,Naoto T Ueno

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CIKS/ACT1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CIKS/ACT1 antibody (AB137395)

Immunocytochemistry/ Immunofluorescence - Anti-CIKS/ACT1 antibody (AB137395)

Western blot - Anti-CIKS/ACT1 antibody (AB137395)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Product promise