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AB250671

Anti-CINP antibody [EPR14445] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CINP antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Cyclin-dependent kinase 2-interacting protein, CDK2-interacting protein, CINP

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-CINP antibody [EPR14445] - BSA and Azide free (AB250671)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CINP antibody [EPR14445] - BSA and Azide free (AB250671)

This data was developed using ab183519, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 20°C Acetone fixed HeLa cells labeling CINP with ab183519 at 1/100 dilution (green). Secondary antibody : Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution. Right panel is counterstained with Dapi (blue).

Immunoprecipitation - Anti-CINP antibody [EPR14445] - BSA and Azide free (AB250671)
  • IP

Supplier Data

Immunoprecipitation - Anti-CINP antibody [EPR14445] - BSA and Azide free (AB250671)

This data was developed using ab183519, the same antibody clone in a different buffer formulation.

Western blot analysis of CINP in immunoprecipitation pellets from 293 lysate.

Lane 1 : ab183159 used at a 1/50 dilution.

Lane 2 : TBS negative control.

Secondary antibody : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.

All lanes:

Immunoprecipitation - Anti-CINP antibody [EPR14445] - N-terminal (<a href='/en-us/products/primary-antibodies/cinp-antibody-epr14445-n-terminal-ab183519'>ab183519</a>)

Predicted band size: 24 kDa

false

Western blot - Anti-CINP antibody [EPR14445] - BSA and Azide free (AB250671)
  • WB

Supplier Data

Western blot - Anti-CINP antibody [EPR14445] - BSA and Azide free (AB250671)

This data was developed using ab183519, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CINP antibody [EPR14445] - N-terminal (<a href='/en-us/products/primary-antibodies/cinp-antibody-epr14445-n-terminal-ab183519'>ab183519</a>) at 1/1000 dilution

Lane 1:

293 cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14445

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

AB14734

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Secondary Antibodies

AB150129

Donkey Anti-Goat IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (AB150129)

  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250671 is the carrier-free version of ab183519.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the DNA replication complex, which interacts with two kinases, CDK2 and CDC7, thereby providing a functional and physical link between CDK2 and CDC7 during firing of the origins of replication (PubMed : 16082200, PubMed : 19889979). Regulates ATR-mediated checkpoint signaling in response to DNA damage (PubMed : 16082200, PubMed : 19889979). Part of the 55LCC heterohexameric ATPase complex which is chromatin-associated and promotes replisome proteostasis to maintain replication fork progression and genome stability. Required for replication fork progression, sister chromatid cohesion, and chromosome stability. The ATPase activity is specifically enhanced by replication fork DNA and is coupled to cysteine protease-dependent cleavage of replisome substrates in response to replication fork damage. Uses ATPase activity to process replisome substrates in S-phase, facilitating their proteolytic turnover from chromatin to ensure DNA replication and mitotic fidelity (PubMed : 38554706). As part of 55LCC complex, also involved in the cytoplasmic maturation steps of pre-60S ribosomal particles by promoting the release of shuttling protein RSL24D1/RLP24 from the pre-ribosomal particles (PubMed : 35354024).
See full target information CINP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 18:20230639 PubMed36820063

2023

Glycoprotein non-metastatic melanoma B interacts with epidermal growth factor receptor to regulate neural stem cell survival and differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Yang,Gang Jin,Shihong Chen,Jing Luo,Wei Xu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com