Rabbit Recombinant Monoclonal CINP antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.
Images
![Western blot - Anti-CINP antibody [EPR14445] - BSA and Azide free (AB250671), expandable thumbnail](https://content.abcam.com/products/images/cinp-antibody-epr14445-bsa-and-azide-free-ab250671--western-blot-img105568.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CINP antibody [EPR14445] - BSA and Azide free (AB250671)")
![Immunoprecipitation - Anti-CINP antibody [EPR14445] - BSA and Azide free (AB250671), expandable thumbnail](https://content.abcam.com/products/images/cinp-antibody-epr14445-bsa-and-azide-free-ab250671--immunoprecipitation-img68413.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CINP antibody [EPR14445] - BSA and Azide free (AB250671)")
![Immunocytochemistry/ Immunofluorescence - Anti-CINP antibody [EPR14445] - BSA and Azide free (AB250671), expandable thumbnail](https://content.abcam.com/products/images/cinp-antibody-epr14445-bsa-and-azide-free-ab250671--immunocytochemistry-immunofluorescence-img41744.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CINP antibody [EPR14445] - BSA and Azide free (AB250671)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Component of the DNA replication complex, which interacts with two kinases, CDK2 and CDC7, thereby providing a functional and physical link between CDK2 and CDC7 during firing of the origins of replication (PubMed:16082200, PubMed:19889979). Regulates ATR-mediated checkpoint signaling in response to DNA damage (PubMed:16082200, PubMed:19889979). Part of the 55LCC heterohexameric ATPase complex which is chromatin-associated and promotes replisome proteostasis to maintain replication fork progression and genome stability. Required for replication fork progression, sister chromatid cohesion, and chromosome stability. The ATPase activity is specifically enhanced by replication fork DNA and is coupled to cysteine protease-dependent cleavage of replisome substrates in response to replication fork damage. Uses ATPase activity to process replisome substrates in S-phase, facilitating their proteolytic turnover from chromatin to ensure DNA replication and mitotic fidelity (PubMed:38554706). As part of 55LCC complex, also involved in the cytoplasmic maturation steps of pre-60S ribosomal particles by promoting the release of shuttling protein RSL24D1/RLP24 from the pre-ribosomal particles (PubMed:35354024).
Alternative names
Cyclin-dependent kinase 2-interacting protein, CDK2-interacting protein, CINP
Recommended products
Rabbit Recombinant Monoclonal CINP antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR14445
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250671 is the carrier-free version of Anti-CINP antibody [EPR14445] - N-terminal ab183519.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-CINP antibody [EPR14445] - BSA and Azide free (ab250671), expandable thumbnail](https://content.abcam.com/products/images/cinp-antibody-epr14445-bsa-and-azide-free-ab250671--western-blot-img105568.jpg "Western blot - Anti-CINP antibody [EPR14445] - BSA and Azide free (ab250671)")
Western blot - Anti-CINP antibody [EPR14445] - BSA and Azide free (ab250671)
This data was developed using Anti-CINP antibody [EPR14445] - N-terminal ab183519, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-CINP antibody [EPR14445] - N-terminal (Anti-CINP antibody [EPR14445] - N-terminal ab183519) at 1/1000 dilution
Lane 1: 293 cell lysate at 20 µg
Lane 2: Jurkat cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 24 kDa
![Immunoprecipitation - Anti-CINP antibody [EPR14445] - BSA and Azide free (ab250671), expandable thumbnail](https://content.abcam.com/products/images/cinp-antibody-epr14445-bsa-and-azide-free-ab250671--immunoprecipitation-img68413.jpg "Immunoprecipitation - Anti-CINP antibody [EPR14445] - BSA and Azide free (ab250671)")
Immunoprecipitation - Anti-CINP antibody [EPR14445] - BSA and Azide free (ab250671)
This data was developed using Anti-CINP antibody [EPR14445] - N-terminal ab183519, the same antibody clone in a different buffer formulation.Western blot analysis of CINP in immunoprecipitation pellets from 293 lysate.Lane 1: ab183159 used at a 1/50 dilution.Lane 2: TBS negative control.Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.
All lanes: Immunoprecipitation - Anti-CINP antibody [EPR14445] - N-terminal (Anti-CINP antibody [EPR14445] - N-terminal ab183519)
Predicted band size: 24 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-CINP antibody [EPR14445] - BSA and Azide free (ab250671), expandable thumbnail](https://content.abcam.com/products/images/cinp-antibody-epr14445-bsa-and-azide-free-ab250671--immunocytochemistry-immunofluorescence-img41744.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CINP antibody [EPR14445] - BSA and Azide free (ab250671)")
Immunocytochemistry/ Immunofluorescence - Anti-CINP antibody [EPR14445] - BSA and Azide free (ab250671)
This data was developed using Anti-CINP antibody [EPR14445] - N-terminal ab183519, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 20°C Acetone fixed HeLa cells labeling CINP with Anti-CINP antibody [EPR14445] - N-terminal ab183519 at 1/100 dilution (green). Secondary antibody: Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution. Right panel is counterstained with Dapi (blue).
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