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AB128179

Anti-CIP2A antibody [2A1-9A4]

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(1 Publication)

Mouse Monoclonal CIP2A antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CIP2A.

View Alternative Names

KIAA1524, CIP2A, Protein CIP2A, Cancerous inhibitor of PP2A, p90 autoantigen

2 Images
Immunoprecipitation - Anti-CIP2A antibody [2A1-9A4] (AB128179)
  • IP

Supplier Data

Immunoprecipitation - Anti-CIP2A antibody [2A1-9A4] (AB128179)

293T whole cell lysate (1 mg for IP, 20% of IP loaded) was used for immunoprecipitation with ab128179 at 3 μg/mg (lane 1) and control IgG (lane 2). Western blotting was performed using ab128179 at 1/1000 dilution. Detection by chemiluminescence was performed for 10 seconds.

All lanes:

Immunoprecipitation - Anti-CIP2A antibody [2A1-9A4] (ab128179)

Predicted band size: 102 kDa

false

Western blot - Anti-CIP2A antibody [2A1-9A4] (AB128179)
  • WB

Supplier Data

Western blot - Anti-CIP2A antibody [2A1-9A4] (AB128179)

Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Western blot - Anti-CIP2A antibody [2A1-9A4] (ab128179) at 1/1000 dilution

Lane 1:

HeLa Whole cell lysate at 15 µg

Lane 2:

HEK293T Whole cell lysate at 15 µg

Lane 3:

Jurkat Whole cell lysate at 15 µg

Lane 4:

mouse TCMK-1 Whole cell lysate at 15 µg

Secondary

All lanes:

HRP-conjugated goat anti-mouse IgG

Predicted band size: 102 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2A1-9A4

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human, Mouse

Applications

IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human CIP2A. The exact immunogen used to generate this antibody is proprietary information.

Q8TCG1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/25000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/25000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Purification notes
The antibody was purified using a mouse immunoglobulin binding ligand.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as an inhibitor of protein phosphatase PP2A (PubMed : 17632056). Promotes anchorage-independent cell growth and tumor formation by preventing dephosphorylation of MYC, thereby stabilizing MYC in human malignancies (PubMed : 17632056). Together with TOPBP1, plays an essential role in the response to genome instability generated by the presence of acentric chromosome fragments derived from shattered chromosomes within micronuclei (PubMed : 35121901, PubMed : 35842428, PubMed : 37165191, PubMed : 37316668). Micronuclei, which are frequently found in cancer cells, consist of chromatin surrounded by their own nuclear membrane : following breakdown of the micronuclear envelope, a process associated with chromothripsis, the CIP2A-TOPBP1 complex tethers chromosome fragments during mitosis to ensure clustered segregation of the fragments to a single daughter cell nucleus, facilitating re-ligation with limited chromosome scattering and loss (PubMed : 37165191, PubMed : 37316668).
See full target information CIP2A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 17:3211-3218 PubMed30867751

2019

MicroRNA-218 inhibits the cell proliferation and migration in clear cell renal cell carcinoma through targeting cancerous inhibitor of protein phosphatase 2A.

Applications

Unspecified application

Species

Unspecified reactive species

Ruojing Wei,Xiongjun Ye,Yawei Zhao,Ning Jia,Tongwei Liu,Wenfeng Lian,Hongjian Wei,Gang Zhang,Lijie Song
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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