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Rabbit Recombinant Monoclonal CIP4 antibody. Carrier free. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-Cip4 antibody [EPR1966(2)] - BSA and Azide free (AB247615), expandable thumbnail
  • Western blot - Anti-Cip4 antibody [EPR1966(2)] - BSA and Azide free (AB247615), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Target data

Function

Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL.

Alternative names

CIP4, STOT, STP, TRIP10, Cdc42-interacting protein 4, Protein Felic, Salt tolerant protein, Thyroid receptor-interacting protein 10, hSTP, TR-interacting protein 10, TRIP-10

Recommended products

Rabbit Recombinant Monoclonal CIP4 antibody. Carrier free. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR1966(2)
Purification technique
Affinity purification Protein A
Specificity

The antibody has been tested in our lab in mouse and rat species without satisfactory results. Therefore, we do not recommend this antibody in these species.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab247615 is the carrier-free version of Anti-Cip4 antibody [EPR1966(2)] ab108277.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Cip4 also known as Cdc42-interacting protein 4 is a member of the F-BAR family and is involved in cellular processes related to actin dynamics. It has a molecular mass of approximately 70 kDa. Cip4 interacts with Cdc42 a small GTPase to facilitate membrane bending and invagination important for endocytosis and membrane trafficking. This protein is expressed in a variety of tissues including the brain heart and liver reflecting its broad functional significance across different cell types.

Biological function summary

Cip4 plays an essential role in linking membranes to the actin cytoskeleton. It is part of a complex with other proteins like Toca-1 and N-WASP which are important for actin polymerization. This function supports cellular morphogenesis and migration by organizing the actin filaments in response to external signals. Cip4 also contributes to the regulation of cell shape and motility aiding in processes such as development and immune responses by modulating membrane-associated actin assembly.

Pathways

Cip4 participates in pathways including the Cdc42 signaling pathway and endocytosis. In the Cdc42 signaling pathway Cip4 works with proteins like N-WASP and dynamin to facilitate membrane remodeling and cytoskeletal rearrangements impacting processes like cell division and signal transduction. Its involvement in endocytosis influences cargo uptake and intracellular transport linking it to dynamin-dependent endocytic pathways critical for nutrient uptake and receptor internalization.

Associated diseases and disorders

Cip4 is associated with certain cancers and neurological disorders. Its dysregulation can contribute to tumor progression often involving interactions with proteins like Cdc42 and N-WASP that promote changes in cell adhesion and motility. Additionally abnormalities in Cip4 expression or function may lead to disrupted cell signaling in neurological disorders impacting processes essential for brain development and function. Understanding Cip4's role in these contexts provides insights into potential therapeutic targets for treatment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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