Rabbit Recombinant Monoclonal CIRP antibody. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 2 publications.
Images
![Immunoprecipitation - Anti-CIRP antibody [EPR23628-36] (AB246510), expandable thumbnail](https://content.abcam.com/products/images/cirp-antibody-epr23628-36-ab246510--immunoprecipitation-img11726.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CIRP antibody [EPR23628-36] (AB246510)")
![Western blot - Anti-CIRP antibody [EPR23628-36] (AB246510), expandable thumbnail](https://content.abcam.com/products/images/cirp-antibody-epr23628-36-ab246510--western-blot-img13494.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CIRP antibody [EPR23628-36] (AB246510)")
![Western blot - Anti-CIRP antibody [EPR23628-36] (AB246510), expandable thumbnail](https://content.abcam.com/products/images/cirp-antibody-epr23628-36-ab246510--western-blot-img55955.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CIRP antibody [EPR23628-36] (AB246510)")
![Western blot - Anti-CIRP antibody [EPR23628-36] (AB246510), expandable thumbnail](https://content.abcam.com/products/images/cirp-antibody-epr23628-36-ab246510--western-blot-img428640.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CIRP antibody [EPR23628-36] (AB246510)")
Publications
Filter by
- iScience 26:1070432023Targeting extracellular CIRP with an X-aptamer shows therapeutic potential in acute pancreatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWuming Liu,Jianbin Bi,Yifan Ren,Huan Chen,Jia Zhang,Tao Wang,Mengzhou Wang,Lin Zhang,Junzhou Zhao,Zheng Wu,Yi Lv,Bing Liu,Rongqian WuPubMed 37360693
- BioFactors (Oxford, England) 47:427-4432021Human antigen R (HuR) and Cold inducible RNA-binding protein (CIRP) influence intestinal mucosal barrier function in ulcerative colitis by competitive regulation on Claudin1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Xu,Yuxi Tian,Ying Wang,Junwen Yang,Fujun Li,Xiaoping Wan,Miao OuyangPubMed 33638934
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Expected | Not recommended | Tested | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Promotes assembly of stress granules (SGs), when overexpressed. Seems to play an essential role in cold-induced suppression of cell proliferation. Acts as a translational repressor. Acts as a translational activator. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN.
Alternative names
Cold-inducible RNA-binding protein, A18 hnRNP, Glycine-rich RNA-binding protein CIRP, Cirp, Cirbp
Recommended products
Rabbit Recombinant Monoclonal CIRP antibody. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 2 publications.
Alternative names
Cold-inducible RNA-binding protein, A18 hnRNP, Glycine-rich RNA-binding protein CIRP, Cirp, Cirbp
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR23628-36
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-CIRP antibody [EPR23628-36] (ab246510), expandable thumbnail](https://content.abcam.com/products/images/cirp-antibody-epr23628-36-ab246510--immunoprecipitation-img11726.jpg "Immunoprecipitation - Anti-CIRP antibody [EPR23628-36] (ab246510)")
Immunoprecipitation - Anti-CIRP antibody [EPR23628-36] (ab246510)
CIRBP was immunoprecipitated from 0.35 mg Mouse testis tissue lysate with ab246510 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246510 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse testis tissue lysate 10ug
Lane 2: ab246510 IP in Mouse testis tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab246510 in Mouse testis tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 47 seconds.
This blot was developed using a higher sensitivity ECL substrate.
All lanes: Immunoprecipitation - Anti-CIRP antibody [EPR23628-36] (ab246510)
Predicted band size: 18 kDa
Observed band size: 18 kDa
![Western blot - Anti-CIRP antibody [EPR23628-36] (ab246510), expandable thumbnail](https://content.abcam.com/products/images/cirp-antibody-epr23628-36-ab246510--western-blot-img13494.jpg "Western blot - Anti-CIRP antibody [EPR23628-36] (ab246510)")
Western blot - Anti-CIRP antibody [EPR23628-36] (ab246510)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Western blot - Anti-CIRP antibody [EPR23628-36] (ab246510) at 1/1000 dilution
Lane 1: Mouse testis tissue lysate at 20 µg
Lane 2: Rat testis tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
![Western blot - Anti-CIRP antibody [EPR23628-36] (ab246510), expandable thumbnail](https://content.abcam.com/products/images/cirp-antibody-epr23628-36-ab246510--western-blot-img55955.jpg "Western blot - Anti-CIRP antibody [EPR23628-36] (ab246510)")
Western blot - Anti-CIRP antibody [EPR23628-36] (ab246510)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
This antibody also recognized an undetermined band at around 50 kDa
Exposure time: 3 minutes.
All lanes: Western blot - Anti-CIRP antibody [EPR23628-36] (ab246510) at 1/1000 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast) treated at 37°C for 48 hours, whole cell lysate at 20 µg
Lane 2: NIH/3T3 treated at 32°C for 48 hours, whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
![Western blot - Anti-CIRP antibody [EPR23628-36] (ab246510), expandable thumbnail](https://content.abcam.com/products/images/cirp-antibody-epr23628-36-ab246510--western-blot-img428640.jpg "Western blot - Anti-CIRP antibody [EPR23628-36] (ab246510)")
Western blot - Anti-CIRP antibody [EPR23628-36] (ab246510)
Blocking and dilution buffer: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate.
All lanes: Western blot - Anti-CIRP antibody [EPR23628-36] (ab246510) at 1/1000 dilution
Lane 1: Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 2: NIH/3T3 (Mouse embryonic fibroblast) treated with 32˚C for 48 h whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 180s
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