Anti-CIRP antibody [EPR23628-36]

• IP

Unknown

Immunoprecipitation - Anti-CIRP antibody [EPR23628-36] (AB246510)

CIRBP was immunoprecipitated from 0.35 mg Mouse testis tissue lysate with ab246510 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246510 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse testis tissue lysate 10ug

Lane 2 : ab246510 IP in Mouse testis tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab246510 in Mouse testis tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 47 seconds.

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Immunoprecipitation - Anti-CIRP antibody [EPR23628-36] (ab246510)

Predicted band size: 18 kDa

Observed band size: 18 kDa

false

• WB

Lab

Western blot - Anti-CIRP antibody [EPR23628-36] (AB246510)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

This antibody also recognized an undetermined band at around 50 kDa

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-CIRP antibody [EPR23628-36] (ab246510) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) treated at 37°C for 48 hours, whole cell lysate at 20 µg

Lane 2:

NIH/3T3 treated at 32°C for 48 hours, whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 18 kDa

Observed band size: 18 kDa

false

• WB

Lab

Western blot - Anti-CIRP antibody [EPR23628-36] (AB246510)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-CIRP antibody [EPR23628-36] (ab246510) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 20 µg

Lane 2:

Rat testis tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 18 kDa

Observed band size: 18 kDa

false

• WB

Lab

Western blot - Anti-CIRP antibody [EPR23628-36] (AB246510)

Blocking and dilution buffer : 5% NFDM/TBST.

This blot was developed using a high sensitivity ECL substrate.

All lanes:

Western blot - Anti-CIRP antibody [EPR23628-36] (ab246510) at 1/1000 dilution

Lane 1:

Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast) treated with 32˚C for 48 h whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 18 kDa

Observed band size: 18 kDa

false

Exposure time: 180s