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Knockout Tested Rabbit Recombinant Monoclonal MitoNEET antibody. Suitable for ICC/IF, IHC-P, WB and reacts with Human, Mouse, Rat samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-PWBFlow Cyt (Intra)IP
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Not recommended
Not recommended
Rat
Expected
Tested
Tested
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Species

Mouse

Dilution info

1/100

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/5000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/5000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation (By similarity). May be involved in Fe-S cluster shuttling and/or in redox reactions.

Alternative names

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Knockout Tested Rabbit Recombinant Monoclonal MitoNEET antibody. Suitable for ICC/IF, IHC-P, WB and reacts with Human, Mouse, Rat samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR29116-27

Purification technique

Affinity purification Protein A

Specificity

This antibody weakly cross-react with human CISD2 overexpression lysate.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized CISD1 KO HeLa (human cervical adenocarcinoma epithelial cell), ab265276 cells labelling MitoNEET with ab318199 at 1/100 (5.1 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing mitochondrial staining in wildtype HeLa cells (shown in green), and negative staining in CISD1 knockout HeLa cells. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain tubulin at 1/1000 1ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: ab318199 at 1/100 (5.1 ug/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 2 ug/mL dilution.

    -ve control 2: Anti-COX IV mouse monoclonal 1/1000 1ug/ml dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling MitoNEET with ab318199 at 1/100 (5.1 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing mitochondrial staining in NIH/3T3 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain tubulin at 1/1000 1ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: ab318199 at 1/100 (5.1 ug/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 2 ug/mL dilution.

    -ve control 2: Anti-COX IV mouse monoclonal 1/1000 1ug/ml dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199)

    Immunohistochemical analysis of paraffin-embedded (A) Wild-type Hela (human cervical adenocarcinoma epithelial cell) cell pellet; (B) CISD1 knockout Hela cell pellet tissue labeling MitoNEET with ab318199 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on (A) wild-type Hela cell pellet, no staining on (B) human CISD1 knockout Hela cell pellet. The section was incubated with ab318199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199)

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling MitoNEET with ab318199 at 1/5000 (0.102 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat colon. The section was incubated with ab318199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling MitoNEET with ab318199 at 1/5000 (0.102 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat liver. The section was incubated with ab318199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199)

    Immunohistochemical analysis of paraffin-embedded Mouse liver cancer tissue labeling MitoNEET with ab318199 at 1/5000 (0.102 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse liver cancer. The section was incubated with ab318199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling MitoNEET with ab318199 at 1/5000 (0.102 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse colon. The section was incubated with ab318199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling MitoNEET with ab318199 at 1/5000 (0.102 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse liver. The section was incubated with ab318199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199)

    Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling MitoNEET with ab318199 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human ovarian carcinoma. The section was incubated with ab318199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MitoNEET with ab318199 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human colon. The section was incubated with ab318199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling MitoNEET with ab318199 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human cerebrum. The section was incubated with ab318199 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199), expandable thumbnail

    Western blot - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199)

    All lanes: Western blot - Anti-CISD1/MitoNEET antibody [EPR29116-27] (AB318199) at 1/1000 dilution

    Lane 1: Human cerebellum tissue lysate at 20 µg

    Lane 2: Mouse cerebellum tissue lysate at 20 µg

    Lane 3: Mouse liver tissue lysate at 20 µg

    Lane 4: Mouse kidney tissue lysate at 20 µg

    Lane 5: Rat cerebellum tissue lysate at 20 µg

    Lane 6: Rat liver tissue lysate at 20 µg

    Lane 7: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 15 kDa

    Exposure time: 8s

  • Western blot - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199), expandable thumbnail

    Western blot - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-AIF antibody [E20] (ab32516) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-CISD1/MitoNEET antibody [EPR29116-27] (AB318199) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) cytoplasmic fraction at 20 µg

    Lane 2: HeLa mitochondrial fraction at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 15 kDa, 36 kDa, 67 kDa

    Exposure time: 10s

  • Western blot - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199), expandable thumbnail

    Western blot - Anti-CISD1/MitoNEET antibody [EPR29116-27] (ab318199)

    In Western blot, ab318199 was shown to bind specifically to CISD1. Target of interest was observed at 15 kDa in wild-type Hela cell lysates (lane 1) with no signal observed at this size in CISD1 knockout cell line (lane 2) (lane 2, knockout cell line ab265276 / knockout cell lysate ab258817).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

    Exposure time: Lane 1-2: 114 seconds, Lane 3-5: 59 seconds

    All lanes: Western blot - Anti-CISD1/MitoNEET antibody [EPR29116-27] (AB318199) at 1/1000 dilution

    Lane 1: Wild-type HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: CISD1 knockout HeLa whole cell lysate at 20 µg

    Lane 3: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 4: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 5: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Performed under reducing conditions.

    Observed band size: 15 kDa, 36 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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