Rabbit Recombinant Monoclonal CITED2 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 8 publications.
Images
![Western blot - Anti-CITED2 antibody [EPR3416(2)] (AB108345), expandable thumbnail](https://content.abcam.com/products/images/cited2-antibody-epr34162-ab108345--western-blot-img93601.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CITED2 antibody [EPR3416(2)] (AB108345)")
![Western blot - Anti-CITED2 antibody [EPR3416(2)] (AB108345), expandable thumbnail](https://content.abcam.com/products/images/cited2-antibody-epr34162-ab108345--western-blot-img83677.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CITED2 antibody [EPR3416(2)] (AB108345)")
![Western blot - Anti-CITED2 antibody [EPR3416(2)] (AB108345), expandable thumbnail](https://content.abcam.com/products/images/cited2-antibody-epr34162-ab108345--western-blot-img152312.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CITED2 antibody [EPR3416(2)] (AB108345)")
![Western blot - Anti-CITED2 antibody [EPR3416(2)] (AB108345), expandable thumbnail](https://content.abcam.com/products/images/cited2-antibody-epr34162-ab108345--western-blot-img105329.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CITED2 antibody [EPR3416(2)] (AB108345)")
Publications
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- The Journal of biological chemistry 294:8617-86292019The pro-death role of Cited2 in stroke is regulated by E2F1/4 transcription factors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianwen Huang et. al.PubMed 30967472
- International journal of oncology 54:2019-20292019MAT2B promotes proliferation and inhibits apoptosis in osteosarcoma by targeting epidermal growth factor receptor and proliferating cell nuclear antigen.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Yuan et. al.PubMed 30942439
- The Journal of biological chemistry 293:11109-111182018The transcription factor Vezf1 represses the expression of the antiangiogenic factor Cited2 in endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLama AlAbdi et. al.PubMed 29794136
- Oncotarget 9:6707-67172018Endoplasmic reticulum stress stimulates the release of extracellular vesicles carrying danger-associated molecular pattern (DAMP) molecules.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGavin P Collett et. al.PubMed 29467921
- Nature communications 7:131472016The GCN5-CITED2-PKA signalling module controls hepatic glucose metabolism through a cAMP-induced substrate switch.
- The Journal of neuroscience : the official journal of the Society for Neuroscience 36:6403-192016Cited2 Regulates Neocortical Layer II/III Generation and Somatosensory Callosal Projection Neuron Development and Connectivity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyann M Fame,Jessica L MacDonald,Sally L Dunwoodie,Emi Takahashi,Jeffrey D MacklisPubMed 27307230
- Reproduction (Cambridge, England) 151:509-162016CITED2 modulation of trophoblast cell differentiation: insights from global transcriptome analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazuhiko Imakawa,Pramod Dhakal,Kaiyu Kubota,Kazuya Kusama,Damayanti Chakraborty,M A Karim Rumi,Michael J SoaresPubMed 26917451
- PloS one 10:e01370792015High-Throughput siRNA Screening to Reveal GATA-2 Upstream Transcriptional Mechanisms in Hematopoietic Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYo Saito,Tohru Fujiwara,Keiichi Ohashi,Yoko Okitsu,Noriko Fukuhara,Yasushi Onishi,Kenichi Ishizawa,Hideo HarigaePubMed 26325290
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Not recommended | Tested |
| Mouse | Not recommended | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Transcriptional coactivator of the p300/CBP-mediated transcription complex. Acts as a bridge, linking TFAP2 transcription factors and the p300/CBP transcriptional coactivator complex in order to stimulate TFAP2-mediated transcriptional activation. Positively regulates TGF-beta signaling through its association with the SMAD/p300/CBP-mediated transcriptional coactivator complex. Stimulates the peroxisome proliferator-activated receptors PPARA transcriptional activity. Enhances estrogen-dependent transactivation mediated by estrogen receptors. Acts also as a transcriptional corepressor; interferes with the binding of the transcription factors HIF1A or STAT2 and the p300/CBP transcriptional coactivator complex. Participates in sex determination and early gonad development by stimulating transcription activation of SRY. Plays a role in controlling left-right patterning during embryogenesis; potentiates transcriptional activation of NODAL-mediated gene transcription in the left lateral plate mesoderm (LPM). Plays an essential role in differentiation of the adrenal cortex from the adrenogonadal primordium (AGP); stimulates WT1-mediated transcription activation thereby up-regulating the nuclear hormone receptor NR5A1 promoter activity. Associates with chromatin to the PITX2 P1 promoter region.
Alternative names
MRG1, CITED2, Cbp/p300-interacting transactivator 2, MSG-related protein 1, P35srj, MRG-1
Recommended products
Rabbit Recombinant Monoclonal CITED2 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 8 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR3416(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Stable for 12 months at -20°C
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-CITED2 antibody [EPR3416(2)] (ab108345), expandable thumbnail](https://content.abcam.com/products/images/cited2-antibody-epr34162-ab108345--western-blot-img93601.jpg "Western blot - Anti-CITED2 antibody [EPR3416(2)] (ab108345)")
Western blot - Anti-CITED2 antibody [EPR3416(2)] (ab108345)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-CITED2 antibody [EPR3416(2)] (ab108345) at 1/2000 dilution
All lanes: NIH/3T3 whole cell lysate at 10 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 103 kDa, 21 kDa, 28 kDa, 42 kDa, 68 kDa
Observed band size: 120 kDa, 28 kDa, 42 kDa, 74 kDa
![Western blot - Anti-CITED2 antibody [EPR3416(2)] (ab108345), expandable thumbnail](https://content.abcam.com/products/images/cited2-antibody-epr34162-ab108345--western-blot-img83677.jpg "Western blot - Anti-CITED2 antibody [EPR3416(2)] (ab108345)")
Western blot - Anti-CITED2 antibody [EPR3416(2)] (ab108345)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-CITED2 antibody [EPR3416(2)] (ab108345) at 20 µg
All lanes: Saos-2 whole cell lysate at 20 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 28 kDa, 53 kDa, 94 kDa
Observed band size: 100 kDa, 28 kDa, 50 kDa
![Western blot - Anti-CITED2 antibody [EPR3416(2)] (ab108345), expandable thumbnail](https://content.abcam.com/products/images/cited2-antibody-epr34162-ab108345--western-blot-img152312.jpg "Western blot - Anti-CITED2 antibody [EPR3416(2)] (ab108345)")
Western blot - Anti-CITED2 antibody [EPR3416(2)] (ab108345)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-CITED2 antibody [EPR3416(2)] (ab108345) at 1/1000 dilution
All lanes: U937 whole cell lysate at 20 µg
SecondaryAll lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
![Western blot - Anti-CITED2 antibody [EPR3416(2)] (ab108345), expandable thumbnail](https://content.abcam.com/products/images/cited2-antibody-epr34162-ab108345--western-blot-img105329.jpg "Western blot - Anti-CITED2 antibody [EPR3416(2)] (ab108345)")
Western blot - Anti-CITED2 antibody [EPR3416(2)] (ab108345)
All lanes: Western blot - Anti-CITED2 antibody [EPR3416(2)] (ab108345) at 1/1000 dilution
Lane 1: Saos-2 cell lysate at 10 µg
Lane 2: U937 cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Predicted band size: 28 kDa
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