AB128564

Anti-Citrate synthetase antibody [2H8BB6]

Name: Anti-Citrate synthetase antibody [2H8BB6] (ab128564) | Abcam
Brand: Abcam Limited
SKU: AB128564
Price: 485 USD
Availability: InStock

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(3 Publications)

Mouse Monoclonal Citrate synthetase antibody. Suitable for IP, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Citrate (Si)-synthase, CS

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Citrate synthetase antibody [2H8BB6] (AB128564)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Citrate synthetase antibody [2H8BB6] (AB128564)

Immunofluorescent staining of Citrate synthetase in Hela cells (human) using ab128564.

The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were then incubated with the antibody (ab128564, 5µg/ml) for 2h at room temperature or overnight at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix.

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Immunoprecipitation - Anti-Citrate synthetase antibody [2H8BB6] (AB128564)

Immunoprecipitation using ab128564

Lane 1 : Ladder
Lane 2 : Human Liver Mitochondria

All lanes:

Immunoprecipitation - Anti-Citrate synthetase antibody [2H8BB6] (ab128564)

Predicted band size: 51 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2H8BB6

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IP, ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Precipitation Ammonium Sulphate

Purification notes

Purity is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: 99% HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Citrate synthetase also known as citrate synthase is an important enzyme in the tricarboxylic acid cycle. It catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and CoA. The enzyme weighs around 49 kilodaltons. It is expressed prominently in the mitochondria of eukaryotic cells where it initiates the Krebs cycle by providing citrate to be further processed. Citrate synthetase is an important point of control within this metabolic cycle.

Biological function summary

Citrate synthetase plays a central role in energy production by converting oxaloacetate and acetyl-CoA into citrate. This enzyme is not known to be part of any larger complex but associates with downstream enzymes such as aconitase in the metabolic pathway. Its activity is essential for cellular respiration impacting the overall metabolic rate of the organism. The concentration of citrate produced serves as a checkpoint both for the continuation of the Krebs cycle and for feedback inhibition of glycolysis.

Pathways

Citrate synthetase is integral to the Krebs cycle and the related oxidative phosphorylation pathway. These pathways are essential for efficient ATP production. Citrate synthetase works closely with enzymes like isocitrate dehydrogenase and alpha-ketoglutarate dehydrogenase within the Krebs cycle. Through these interactions citrate synthetase ensures the proper flow of carbon through the cycle impacting ATP yield and cellular energy homeostasis.

Citrate synthetase's function can influence metabolic diseases like diabetes and mitochondrial disorders. Alterations in its activity may contribute to the dysregulation of glucose metabolism seen in diabetes affecting enzymes like glucose transporter 4 (GLUT4). In mitochondrial disorders changes in its normal activity can cause energy production deficiencies influencing proteins such as cytochrome c which is critical in the electron transport chain. Understanding these interactions can help develop therapeutic strategies targeting metabolic pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key enzyme of the Krebs tricarboxylic acid cycle which catalyzes the synthesis of citrate from acetyl coenzyme A and oxaloacetate.

See full target information Citrate synthase, mitochondrial

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e22650 PubMed36394523

2022

Eradication of HCV by direct antiviral agents restores mitochondrial function and energy homeostasis in peripheral blood mononuclear cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rosanna Villani,Moris Sangineto,Paola Pontrelli,Francesco Bellanti,Vidyasagar N Bukke,Archana Moola,Loreto Gesualdo,Gianluigi Vendemiale,Giuseppe Grandaliano,Giovanni Stallone,Gaetano Serviddio

Scientific reports 8:9817 PubMed29959348

2018

Increased nuclear DNA damage precedes mitochondrial dysfunction in peripheral blood mononuclear cells from Huntington's disease patients.

Applications

Species

Human

Georgina Askeland,Zaneta Dosoudilova,Marie Rodinova,Jiri Klempir,Irena Liskova,Anna Kuśnierczyk,Magnar Bjørås,Gaute Nesse,Arne Klungland,Hana Hansikova,Lars Eide

FEBS letters 591:1641-1652 PubMed28391595

2017

Human METTL12 is a mitochondrial methyltransferase that modifies citrate synthase.

Applications

Unspecified application

Species

Unspecified reactive species

Virginie F Rhein,Joe Carroll,Shujing Ding,Ian M Fearnley,John E Walker

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Citrate synthetase antibody [2H8BB6]

Immunocytochemistry/ Immunofluorescence - Anti-Citrate synthetase antibody [2H8BB6] (AB128564)

Immunoprecipitation - Anti-Citrate synthetase antibody [2H8BB6] (AB128564)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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