AB96600

Anti-Citrate synthetase antibody

Name: Anti-Citrate synthetase antibody (ab96600) | Abcam
Brand: Abcam Limited
SKU: AB96600
Price: 460 USD
Availability: InStock
Rating: 5 (11 reviews)

(11 Reviews)

(185 Publications)

Anti-Citrate synthetase antibody (ab96600) is a rabbit polyclonal antibody detecting Citrate synthetase in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat, Zebrafish.

- Over 140 publications
- Trusted since 2010

View Alternative Names

Citrate (Si)-synthase, CS

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-Citrate synthetase antibody (AB96600)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Citrate synthetase antibody (AB96600)

Immunofluorescence analysis of 4% paraformaldehyde fixed at RT for 15 mins HeLa cells labelling Citrate synthetase protein at mitochondria using ab96600 at a 1/500 dilution (Green). The nuclear counterstain is DAPI (Blue). Scale bar= 10 µm.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (AB96600)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (AB96600)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse muscle tissue labeling Citrate synthetase with ab96600 at a dilution of 1/500.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (AB96600)

Paraffin-embedded rat heart tissue stained for Citrate synthetase protein at mitochondria with ab96600 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (AB96600)

Paraffin-embedded mouse brain tissue stained for Citrate synthetase protein at mitochondria with ab96600 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (AB96600)

Paraffin-embedded rat testis tissue stained for Citrate synthetase protein at mitochondria with ab96600 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (AB96600)

Paraffin-embedded mouse kidney tissue stained for Citrate synthetase protein at mitochondria with ab96600 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (AB96600)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse intestine tissue labeling Citrate synthetase with ab96600 at a dilution of 1/500.

Antigen Retrieval : EDTA based buffer, 15 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (AB96600)

Paraffin-embedded zebrafish by whole mount tissue stained for Citrate synthase protein with ab96600 at a 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Zebrafish

Applications

WB, IP, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Citrate synthase, mitochondrial aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

O75390

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-Citrate synthetase antibody (ab96600) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat, Zebrafish samples.

What is the molecular weight of Citrate synthetase?
Anti-Citrate synthetase (ab96600) specifically detects a band for Citrate synthetase (UniProt: O75390) at a molecular weight of 52kDa.

Trusted by the scientific community
Anti-Citrate synthetase (ab96600) was first used in a scientific publication in 2010 and has been cited over 140 times in peer-reviewed journals.

Reviewed by scientists
Anti-Citrate synthetase (ab96600) has over 10 independent reviews from customers.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Citrate synthetase also known as citrate synthase is an important enzyme in the tricarboxylic acid cycle. It catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and CoA. The enzyme weighs around 49 kilodaltons. It is expressed prominently in the mitochondria of eukaryotic cells where it initiates the Krebs cycle by providing citrate to be further processed. Citrate synthetase is an important point of control within this metabolic cycle.

Biological function summary

Citrate synthetase plays a central role in energy production by converting oxaloacetate and acetyl-CoA into citrate. This enzyme is not known to be part of any larger complex but associates with downstream enzymes such as aconitase in the metabolic pathway. Its activity is essential for cellular respiration impacting the overall metabolic rate of the organism. The concentration of citrate produced serves as a checkpoint both for the continuation of the Krebs cycle and for feedback inhibition of glycolysis.

Pathways

Citrate synthetase is integral to the Krebs cycle and the related oxidative phosphorylation pathway. These pathways are essential for efficient ATP production. Citrate synthetase works closely with enzymes like isocitrate dehydrogenase and alpha-ketoglutarate dehydrogenase within the Krebs cycle. Through these interactions citrate synthetase ensures the proper flow of carbon through the cycle impacting ATP yield and cellular energy homeostasis.

Citrate synthetase's function can influence metabolic diseases like diabetes and mitochondrial disorders. Alterations in its activity may contribute to the dysregulation of glucose metabolism seen in diabetes affecting enzymes like glucose transporter 4 (GLUT4). In mitochondrial disorders changes in its normal activity can cause energy production deficiencies influencing proteins such as cytochrome c which is critical in the electron transport chain. Understanding these interactions can help develop therapeutic strategies targeting metabolic pathways.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Key enzyme of the Krebs tricarboxylic acid cycle which catalyzes the synthesis of citrate from acetyl coenzyme A and oxaloacetate.

See full target information Citrate synthase, mitochondrial

style

left-column

Publications (185)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113449 PubMed41050429

2025

Exploring multiorgan mitochondrial dysfunction in the switch toward progressive MASLD in AMLN mice.

Applications

Unspecified application

Species

Unspecified reactive species

Marica Meroni,Erika Paolini,Miriam Longo,Michele Battistin,Daniele Dondossola,Michela Ripolone,Laura Napoli,Ettore Mosca,Stefania Corti,Paola Dongiovanni

International journal of molecular sciences 26: PubMed40806782

2025

The Loss of Complex I in Renal Oncocytoma Is Associated with Defective Mitophagy Due to Lysosomal Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Lin,Neal Patel,Lucia Fernandez-Del-Rio,Cristiane Benica,Blake Wilde,Eirini Christodoulou,Shinji Ohtake,Anhyo Jeong,Aboubacar Kaba,Nedas Matulionis,Randy Caliliw,Xiaowu Gai,Heather Christofk,David Shackelford,Brian Shuch

PloS one 20:e0327008 PubMed40577269

2025

Effects of 810 nm treatments in acute myofiber contraction of C2C12 myotubes.

Applications

Unspecified application

Species

Unspecified reactive species

Nashwa Cheema,Linh Pham,Alexa Nazarian,Namrata Ghag,Emma Wise,Christiane Fuchs,Richard Rox Anderson,Joshua Tam

Cell death & disease 16:172 PubMed40074754

2025

Point mutations of the mitochondrial chaperone TRAP1 affect its functions and pro-neoplastic activity.

Applications

Unspecified application

Species

Unspecified reactive species

Claudio Laquatra,Alessia Magro,Federica Guarra,Matteo Lambrughi,Lavinia Ferrone,Giulio Fracasso,Melissa Bacchin,Martina La Spina,Elisabetta Moroni,Elena Papaleo,Giorgio Colombo,Andrea Rasola

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70321 PubMed39853792

2025

Brain-derived neurotrophic factor drives muscle adaptation similar to aerobic training in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander D Brown,Alexander D Marko,Daniel M Marko,Bradley J Baranowski,Sebastian Silvera,Michael S Finch,Alex J Yang,Roopan Dhaliwal,Chantal R Ryan,Brian D Roy,Val A Fajardo,Rebecca E K MacPherson

Scandinavian journal of medicine & science in sports 34:e14768 PubMed39604207

2024

Exercise Training Counteracts Compromised Mitochondrial Capacity Induced by Energy Restriction in Prediabetics in a Sex-Dependent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Magni Mohr,Jerónimo Aragón Vela,May-Britt Skoradal,Martin Thomassen,Søren Andersen Skriver,Mette Hansen,Ioannis G Fatouros,Peter Krustrup,Nikolai B Nordsborg

Cancers 16: PubMed39272828

2024

Mitochondrial VDAC1 Silencing in Urethane-Induced Lung Cancer Inhibits Tumor Growth and Alters Cancer Oncogenic Properties.

Applications

Unspecified application

Species

Unspecified reactive species

Nataly Melnikov,Srinivas Pittala,Anna Shteinfer-Kuzmine,Varda Shoshan-Barmatz

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 21:e00432 PubMed39164165

2024

Integrated elemental analysis supports targeting copper perturbations as a therapeutic strategy in multiple sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

James B W Hilton,Kai Kysenius,Jeffrey R Liddell,Stephen W Mercer,Carsten Rautengarten,Dominic J Hare,Gojko Buncic,Bence Paul,Simon S Murray,Catriona A McLean,Trevor J Kilpatrick,Joseph S Beckman,Scott Ayton,Ashley I Bush,Anthony R White,Blaine R Roberts,Paul S Donnelly,Peter J Crouch

JCI insight 9: PubMed39163131

2024

dldhcri3 zebrafish exhibit altered mitochondrial ultrastructure, morphology, and dysfunction partially rescued by probucol or thiamine.

Applications

Unspecified application

Species

Unspecified reactive species

Manuela Lavorato,Donna Iadarola,Cristina Remes,Prabhjot Kaur,Chynna Broxton,Neal D Mathew,Rui Xiao,Christoph Seiler,Eiko Nakamaru-Ogiso,Vernon E Anderson,Marni J Falk

Clinical kidney journal 17:sfae221 PubMed39145145

2024

Urine metabolite changes after cardiac surgery predict acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Zeng,Jinghan Feng,Xinni Zhang,Fangyuan Peng,Ting Ren,Zhouping Zou,Chao Tang,Qian Sun,Xiaoqiang Ding,Ping Jia

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Citrate synthetase antibody

Immunocytochemistry/ Immunofluorescence - Anti-Citrate synthetase antibody (AB96600)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (AB96600)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (AB96600)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (AB96600)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (AB96600)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (AB96600)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (AB96600)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (AB96600)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (185)

Product promise