Anti-Citrate synthetase antibody (ab96600) is a rabbit polyclonal antibody detecting Citrate synthetase in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat, Zebrafish.
- Over 140 publications
- Trusted since 2010
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- Nutrients 15:2023Essential Amino Acids-Rich Diet Increases Cardiomyocytes Protection in Doxorubicin-Treated Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiovanni Corsetti,Claudia Romano,Evasio Pasini,Tiziano Scarabelli,Carol Chen-Scarabelli,Francesco S DioguardiPubMed 37242170
- Science advances 9:eade78642023Mitochondrial phosphatidylethanolamine modulates UCP1 to promote brown adipose thermogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJordan M Johnson,Alek D Peterlin,Enrique Balderas,Elahu G Sustarsic,J Alan Maschek,Marisa J Lang,Alejandro Jara-Ramos,Vanja Panic,Jeffrey T Morgan,Claudio J Villanueva,Alejandro Sanchez,Jared Rutter,Irfan J Lodhi,James E Cox,Kelsey H Fisher-Wellman,Dipayan Chaudhuri,Zachary Gerhart-Hines,Katsuhiko FunaiPubMed 36827367
- Journal of orthopaedic surgery and research 18:1312023Functional heterogeneity of MCT1 and MCT4 in metabolic reprogramming affects osteosarcoma growth and metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGaohong Sheng,Yuan Gao,Hua Wu,Yang Liu,Yong YangPubMed 36814318
- Journal of translational medicine 21:1322023P2RX7 promotes osteosarcoma progression and glucose metabolism by enhancing c-Myc stabilization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGaohong Sheng,Yuan Gao,Qing Ding,Ruizhuo Zhang,Tianqi Wang,Shaoze Jing,Hongqi Zhao,Tian Ma,Hua Wu,Yong YangPubMed 36803784
- Fluids and barriers of the CNS 19:982022Induced pluripotent stem cell-derived cells model brain microvascular endothelial cell glucose metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCallie M Weber,Bilal Moiz,Sophia M Zic,Viviana Alpízar Vargas,Andrew Li,Alisa Morss ClynePubMed 36494870
- Cells 11:2022Evidences for Mutant Huntingtin Inducing Musculoskeletal and Brain Growth Impairments via Disturbing Testosterone Biosynthesis in Male Huntington Disease Animals.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLibo Yu-Taeger,Arianna Novati,Jonasz Jeremiasz Weber,Elisabeth Singer-Mikosch,Ann-Sophie Pabst,Fubo Cheng,Carsten Saft,Jennifer Koenig,Gisa Ellrichmann,Taneli Heikkinen,Mahmoud A Pouladi,Olaf Riess,Huu Phuc NguyenPubMed 36497038
- The Journal of biological chemistry 298:1025682022Low-dose lithium supplementation promotes adipose tissue browning and sarco(endo)plasmic reticulum Ca ATPase uncoupling in muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMia S Geromella,Chantal R Ryan,Jessica L Braun,Michael S Finch,Lucas A Maddalena,Olivia Bagshaw,Briana L Hockey,Fereshteh Moradi,Rachel K Fenech,Jisook Ryoo,Daniel M Marko,Roopan Dhaliwal,Jake Sweezey-Munroe,Sophie I Hamstra,Georgina Gardner,Sebastian Silvera,Rene Vandenboom,Brian D Roy,Jeffrey A Stuart,Rebecca E K MacPherson,Val A FajardoPubMed 36209826
- Communications biology 5:10342022The VersaLive platform enables microfluidic mammalian cell culture for versatile applications.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiovanni Marco Nocera,Gaetano Viscido,Stefania Criscuolo,Simona Brillante,Fabrizia Carbone,Leopoldo Staiano,Sabrina Carrella,Diego di BernardoPubMed 36175545
- iScience 25:1052302022Metabolomic fingerprinting of renal disease progression in Bardet-Biedl syndrome reveals mitochondrial dysfunction in kidney tubular cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmanuela Marchese,Marianna Caterino,Davide Viggiano,Armando Cevenini,Salvatore Tolone,Ludovico Docimo,Valentina Di Iorio,Francesca Del Vecchio Blanco,Roberta Fedele,Francesca Simonelli,Alessandra Perna,Vincenzo Nigro,Giovambattista Capasso,Margherita Ruoppolo,Miriam ZacchiaPubMed 36281451
- Journal of applied physiology (Bethesda, Md. : 1985) 133:798-8132022Suppression of trimethylamine N-oxide with DMB mitigates vascular dysfunction, exercise intolerance, and frailty associated with a Western-style diet in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVienna E Brunt,Nathan T Greenberg,Zachary J Sapinsley,Abigail G Casso,James J Richey,Nicholas S VanDongen,Rachel A Gioscia-Ryan,Brian P Ziemba,Andrew P Neilson,Kevin P Davy,Douglas R SealsPubMed 35952350
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Citrate synthase, mitochondrial aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link O75390
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Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Tested | Expected |
| Rat | Expected | Tested | Expected | Expected |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted | Predicted |
| Zebrafish | Expected | Tested | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Zebrafish | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Xenopus laevis | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Zebrafish | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Xenopus laevis | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Zebrafish | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Xenopus laevis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Zebrafish | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig, Xenopus laevis | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Key enzyme of the Krebs tricarboxylic acid cycle which catalyzes the synthesis of citrate from acetyl coenzyme A and oxaloacetate.
Alternative names
Citrate (Si)-synthase, CS
Recommended products
Anti-Citrate synthetase antibody (ab96600) is a rabbit polyclonal antibody detecting Citrate synthetase in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat, Zebrafish.
- Over 140 publications
- Trusted since 2010
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Citrate synthase, mitochondrial aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link O75390
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Citrate synthetase antibody (ab96600) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat, Zebrafish samples.
What is the molecular weight of Citrate synthetase?
Anti-Citrate synthetase (ab96600) specifically detects a band for Citrate synthetase (UniProt: O75390) at a molecular weight of 52kDa.
Trusted by the scientific community
Anti-Citrate synthetase (ab96600) was first used in a scientific publication in 2010 and has been cited over 140 times in peer-reviewed journals.
Reviewed by scientists
Anti-Citrate synthetase (ab96600) has over 10 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Citrate synthetase also known as citrate synthase is an important enzyme in the tricarboxylic acid cycle. It catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and CoA. The enzyme weighs around 49 kilodaltons. It is expressed prominently in the mitochondria of eukaryotic cells where it initiates the Krebs cycle by providing citrate to be further processed. Citrate synthetase is an important point of control within this metabolic cycle.
- Biological function summary
Citrate synthetase plays a central role in energy production by converting oxaloacetate and acetyl-CoA into citrate. This enzyme is not known to be part of any larger complex but associates with downstream enzymes such as aconitase in the metabolic pathway. Its activity is essential for cellular respiration impacting the overall metabolic rate of the organism. The concentration of citrate produced serves as a checkpoint both for the continuation of the Krebs cycle and for feedback inhibition of glycolysis.
- Pathways
Citrate synthetase is integral to the Krebs cycle and the related oxidative phosphorylation pathway. These pathways are essential for efficient ATP production. Citrate synthetase works closely with enzymes like isocitrate dehydrogenase and alpha-ketoglutarate dehydrogenase within the Krebs cycle. Through these interactions citrate synthetase ensures the proper flow of carbon through the cycle impacting ATP yield and cellular energy homeostasis.
- Associated diseases and disorders
Citrate synthetase's function can influence metabolic diseases like diabetes and mitochondrial disorders. Alterations in its activity may contribute to the dysregulation of glucose metabolism seen in diabetes affecting enzymes like glucose transporter 4 (GLUT4). In mitochondrial disorders changes in its normal activity can cause energy production deficiencies influencing proteins such as cytochrome c which is critical in the electron transport chain. Understanding these interactions can help develop therapeutic strategies targeting metabolic pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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12 product images
Immunoprecipitation - Anti-Citrate synthetase antibody (ab96600)
Immunoprecipitation of Citrate synthetase from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate using 5 μg of ab96600.
Western blot analysis was performed using ab96600.
All lanes: Immunoprecipitation - Anti-Citrate synthetase antibody (ab96600)
Predicted band size: 51 kDa
Western blot - Anti-Citrate synthetase antibody (ab96600)
10% SDS-PAGE.
All lanes: Western blot - Anti-Citrate synthetase antibody (ab96600) at 1/1000 dilution
Lane 1: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: A431 (Human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Lane 4: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 51 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Citrate synthetase antibody (ab96600)
Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Citrate synthetase (green) with ab96600 at a dilution of 1/200.
Cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. Alpha Tubulin (red) was stained with ab184613 at a dilution of 1/1000. Blue: Hoechst 33342 staining.
Western blot - Anti-Citrate synthetase antibody (ab96600)
10% SDS-PAGE.
All lanes: Western blot - Anti-Citrate synthetase antibody (ab96600) at 1/1000 dilution
All lanes: Rat muscle tissue extracts at 50 µg
Predicted band size: 51 kDa
Western blot - Anti-Citrate synthetase antibody (ab96600)
10% SDS-PAGE.
All lanes: Western blot - Anti-Citrate synthetase antibody (ab96600) at 1/1000 dilution
All lanes: Rat brain tissue extracts at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 51 kDa
Western blot - Anti-Citrate synthetase antibody (ab96600)
10% SDS-PAGE.
All lanes: Western blot - Anti-Citrate synthetase antibody (ab96600) at 1/1000 dilution
All lanes: Mouse brain tissue extracts at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 51 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Citrate synthetase antibody (ab96600)
Immunocytochemistry/Immunofluorescence analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) cells labelling Citrate synthetase (green) with ab96600 at a dilution of 1/500.
Cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. Alpha Tubulin (red) was stained with ab184613 at a dilution of 1/1000. Blue: Hoechst 33342 staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (ab96600)
IHC image of ab96600 staining in human colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96600, 5 μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (ab96600)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse intestine tissue labeling Citrate synthetase with ab96600 at a dilution of 1/500.
Antigen Retrieval: EDTA based buffer, 15 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (ab96600)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse muscle tissue labeling Citrate synthetase with ab96600 at a dilution of 1/500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrate synthetase antibody (ab96600)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of NCI-N87 xenograft tissue labeling Citrate synthetase with ab96600 at a dilution of 1/500.
Antigen Retrieval: EDTA based buffer, 15 mins.
Western blot - Anti-Citrate synthetase antibody (ab96600)
All lanes: Western blot - Anti-Citrate synthetase antibody (ab96600) at 1 µg/mL
Lane 1: Marker
Lane 2: Zebrafish liver homogenate (20ug)
Lane 3: Zebrafish skeletal muscle homogenate (20ug)
Lane 4: Mouse liver mitochondrial homogenate (20ug)
SecondaryAll lanes: Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 47 kDa
Exposure time: 5min
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