AB240908

Anti-Citrulline antibody [6C2.1]

Name: Anti-Citrulline antibody [6C2.1] Mouse monoclonal (ab240908) | Abcam
Brand: Abcam Limited
SKU: AB240908
Price: 410 USD
Availability: InStock

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(5 Publications)

Mouse Monoclonal antibody. Suitable for WB, IHC-P and reacts with Modified Amino Acid samples. Cited in 5 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Citrulline.

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrulline antibody [6C2.1] (AB240908)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrulline antibody [6C2.1] (AB240908)

Formalin-fixed, paraffin-embeded mouse colon tissue stained for Citrulline (Green) using ab240908 at 1/25 dilution for 1 hour at RT in immunohistochemical analysis. Goat Anti-Mouse IgG Alexa Fluor 488 was used as the secondary antibody. The nuclear counterstain is DAPI (Blue).

(A) DAPI (B) ab240908 (C) Composite

Supplier Data

Western blot - Anti-Citrulline antibody [6C2.1] (AB240908)

All lanes:

Western blot - Anti-Citrulline antibody [6C2.1] (ab240908) at 1/1000 dilution

Lane 1:

BSA at 0.5 µg

Lane 2:

BSA at 2 µg

Lane 3:

Citrulline-BSA at 0.5 µg

Lane 4:

Citrulline-BSA at 2 µg

Secondary

All lanes:

Goat Anti-Mouse IgG HRP at 1:2000 for 60 min at RT

true

Exposure time: 5min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6C2.1

Isotype

IgG1

Carrier free

Applications

IHC-P, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Amino Acid": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Citrulline also known as L-citrulline engages in important roles within the nitrogen balance of the body. It is a non-essential amino acid with a molecular weight of about 175.19 g/mol. Citrulline is not incorporated into proteins but is primarily found in liver and intestinal cells. It serves as an intermediate in the urea cycle an important metabolic pathway related to the disposal of excess nitrogen from the body.

Biological function summary

Citrulline plays a significant part in the body's nitrogen cycle and enhances the production of nitric oxide a vital signaling molecule. Citrulline participates in converting ammonia a byproduct from amino acid breakdown into urea in the liver which the body can then excrete. It does not belong to a complex but acts as a precursor to the amino acid arginine enhancing its levels and promoting vasodilation. Citrulline effects also extend to influencing muscle metabolism and cellular energy production.

Pathways

Citrulline participates in the urea cycle and nitric oxide synthesis pathway. It provides a link between ammonia detoxification and the synthesis of arginine. This link enhances nitric oxide availability which is essential for regulating vascular homeostasis and blood pressure. Additionally citrulline relates to the arginine biosynthesis pathway where it aids the conversion to arginine mediated by enzymes such as argininosuccinate synthetase and argininosuccinate lyase. These interactions with enzymes highlight its integration into metabolic pathways central to the organism’s homeostasis.

Citrulline shows involvement in cardiovascular diseases and urea cycle disorders. Citrulline has emerged as a therapeutic marker for cardiovascular diseases by enhancing nitric oxide production and improving endothelial function. Citrulline’s role in urea cycle disorders links it to conditions where the body's ability to detoxify ammonia is compromised. Furthermore citrulline's relationship with arginine impacts both cardiac health and ammonia detoxification integrating it into therapeutic strategies targeting these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Citrulline

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:4960 PubMed37620307

2023

Citrullinated fibrinogen-SAAs complex causes vascular metastagenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yibing Han,Takeshi Tomita,Masayoshi Kato,Norihiro Ashihara,Yumiko Higuchi,Hisanori Matoba,Weiyi Wang,Hikaru Hayashi,Yuji Itoh,Satoshi Takahashi,Hiroshi Kurita,Jun Nakayama,Nobuo Okumura,Sachie Hiratsuka

Journal of thrombosis and haemostasis : JTH 20:2862-2872 PubMed36083779

2022

Citrullinated fibrinogen forms densely packed clots with decreased permeability.

Applications

Unspecified application

Species

Unspecified reactive species

Imre Varjú,Erzsébet Tóth,Ádám Z Farkas,Veronika J Farkas,Erzsébet Komorowicz,Tímea Feller,Balázs Kiss,Miklós Z Kellermayer,László Szabó,András Wacha,Attila Bóta,Colin Longstaff,Krasimir Kolev

Experimental and therapeutic medicine 24:568 PubMed35978928

2022

PAD4 silencing inhibits inflammation whilst promoting trophoblast cell invasion and migration by inactivating the NEMO/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Min Zeng,Minjuan Xu,Xiafang Li,Junying Li,Yuanyuan Liu

Aging cell 21:e13587 PubMed35315217

2022

Acrolein, an endogenous aldehyde induces synaptic dysfunction in vitro and in vivo: Involvement of RhoA/ROCK2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zeyu Zhu,Junfeng Lu,Shuyi Wang,Weijia Peng,Yang Yang,Chen Chen,Xin Zhou,Xifei Yang,Wenjun Xin,Xinyi Chen,Jiakai Pi,Wei Yin,Lin Yao,Rongbiao Pi

Frontiers in plant science 12:638392 PubMed33897727

2021

Citrullination of Proteins as a Specific Response Mechanism in Plants.

Applications

Unspecified application

Species

Unspecified reactive species

Claudius Marondedze,Giuliano Elia,Ludivine Thomas,Aloysius Wong,Chris Gehring

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Citrulline antibody [6C2.1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Citrulline antibody [6C2.1] (AB240908)

Western blot - Anti-Citrulline antibody [6C2.1] (AB240908)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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