AB100932

Anti-Citrulline antibody

Name: Anti-Citrulline antibody (ab100932) | Abcam
Brand: Abcam Limited
SKU: AB100932
Price: 535 USD
Availability: InStock
Rating: 5 (3 reviews)

(3 Reviews)

(15 Publications)

Rabbit Polyclonal antibody. Suitable for ICC, ELISA and reacts with Modified Amino Acid samples. Cited in 15 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Citrulline.

3 Images

AbReview34252****

Immunocytochemistry - Anti-Citrulline antibody (AB100932)

Immunocytochemical analysis of Human buccal mucosal cells, staining Citrulline with ab100932.

Cells were fixed with acetone followed by 2% glutaraldehyde, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 20 minutes at 22°C. Samples were incubated with primary antibody (1/200 in PBS) for 15 hours at 4°C. An undiluted biotinylated anti-rabbit polyclonal IgG was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

ELISA - Anti-Citrulline antibody (AB100932)

ELISA

Supplier Data

ELISA - Anti-Citrulline antibody (AB100932)

ELISA data demonstrating the specificity of ab100932 to citrulline naturally present in histones (endogenous citrulline).

ELISA

Supplier Data

ELISA - Anti-Citrulline antibody (AB100932)

ELISA data showing the high immuno-reactivity of ab100932 against citrulline due to the high amounts of citrulline conjugated to BSA.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Applications

ICC, ELISA

applications

Specificity

ab100932 reacts specifically with intrapeptidic citrulline independently of the amino acid sequence. It does not react with free citrulline, ornithine or arginine. Citrulline is an un-natural amino-acid and its expression is very low. Factors limiting the detection are the amount of endogenous citrulline within a protein and the accessibility of the antibody to these sites.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Amino Acid": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "BSA is recommended for blocking and incubating the antibodies in instead of milk.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Citrulline also known as L-citrulline engages in important roles within the nitrogen balance of the body. It is a non-essential amino acid with a molecular weight of about 175.19 g/mol. Citrulline is not incorporated into proteins but is primarily found in liver and intestinal cells. It serves as an intermediate in the urea cycle an important metabolic pathway related to the disposal of excess nitrogen from the body.

Biological function summary

Citrulline plays a significant part in the body's nitrogen cycle and enhances the production of nitric oxide a vital signaling molecule. Citrulline participates in converting ammonia a byproduct from amino acid breakdown into urea in the liver which the body can then excrete. It does not belong to a complex but acts as a precursor to the amino acid arginine enhancing its levels and promoting vasodilation. Citrulline effects also extend to influencing muscle metabolism and cellular energy production.

Pathways

Citrulline participates in the urea cycle and nitric oxide synthesis pathway. It provides a link between ammonia detoxification and the synthesis of arginine. This link enhances nitric oxide availability which is essential for regulating vascular homeostasis and blood pressure. Additionally citrulline relates to the arginine biosynthesis pathway where it aids the conversion to arginine mediated by enzymes such as argininosuccinate synthetase and argininosuccinate lyase. These interactions with enzymes highlight its integration into metabolic pathways central to the organism’s homeostasis.

Citrulline shows involvement in cardiovascular diseases and urea cycle disorders. Citrulline has emerged as a therapeutic marker for cardiovascular diseases by enhancing nitric oxide production and improving endothelial function. Citrulline’s role in urea cycle disorders links it to conditions where the body's ability to detoxify ammonia is compromised. Furthermore citrulline's relationship with arginine impacts both cardiac health and ammonia detoxification integrating it into therapeutic strategies targeting these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Citrulline

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:4960 PubMed37620307

2023

Citrullinated fibrinogen-SAAs complex causes vascular metastagenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yibing Han,Takeshi Tomita,Masayoshi Kato,Norihiro Ashihara,Yumiko Higuchi,Hisanori Matoba,Weiyi Wang,Hikaru Hayashi,Yuji Itoh,Satoshi Takahashi,Hiroshi Kurita,Jun Nakayama,Nobuo Okumura,Sachie Hiratsuka

Science translational medicine 15:eadd8469 PubMed36630482

2023

Posttranslational modifications induce autoantibodies with risk prediction capability in patients with small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kristin J Lastwika,Andrew Kunihiro,Joell L Solan,Yuzheng Zhang,Lydia R Taverne,David Shelley,Jung-Hyun Rho,Timothy W Randolph,Christopher I Li,Eric L Grogan,Pierre P Massion,Annette L Fitzpatrick,David MacPherson,A McGarry Houghton,Paul D Lampe

Frontiers in immunology 13:849779 PubMed35359951

2022

Characterization of Monoclonal Antibodies Recognizing Citrulline-Modified Residues.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqiong Chen,Lin Weng,Wei Liu,Chenxi Deng,Jinxiu Xuan,Yuan Ma,Cao Li,Jinlu Jiang,Juan Chen,Shengxiang Ge

Journal of thrombosis and haemostasis : JTH 18:2732-2743 PubMed32654410

2020

Quantification of citrullinated histones: Development of an improved assay to reliably quantify nucleosomal H3Cit in human plasma.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte Thålin,Katherina Aguilera,Nathan W Hall,Matthew R Marunde,Jonathan M Burg,Axel Rosell,Maud Daleskog,Maja Månsson,Yohei Hisada,Matthew J Meiners,Zu-Wen Sun,Matthew F Whelihan,Marcus A Cheek,Sarah A Howard,Shruti Saxena-Beem,Denis F Noubouossie,Nigel S Key,Saira Z Sheikh,Michael-Christopher Keogh,Martis W Cowles,Staffan Lundström,Nigel Mackman,Håkan Wallén,Andrea L Johnstone

International journal of molecular sciences 20: PubMed31430907

2019

Galectin-9 Is a Possible Promoter of Immunopathology in Rheumatoid Arthritis by Activation of Peptidyl Arginine Deiminase 4 (PAD-4) in Granulocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Valerie R Wiersma,Alex Clarke,Simon D Pouwels,Elizabeth Perry,Trefa M Abdullah,Clive Kelly,Anthony De Soyza,David Hutchinson,Paul Eggleton,Edwin Bremer

Journal of leukocyte biology 105:1167-1181 PubMed30913315

2019

Pathogenic Entamoeba histolytica, but not Entamoeba dispar, induce neutrophil extracellular trap (NET) formation.

Applications

Unspecified application

Species

Unspecified reactive species

Zayda Fonseca,Eileen Uribe-Querol,César Díaz-Godínez,Julio C Carrero,Carlos Rosales

Scientific reports 8:17917 PubMed30559453

2018

Extracellular vesicles are associated with the systemic inflammation of patients with seropositive rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Catalina Burbano,Mauricio Rojas,Carlos Muñoz-Vahos,Adriana Vanegas-García,Luis A Correa,Gloria Vásquez,Diana Castaño

Cellular immunology 336:1-11 PubMed30538031

2018

Platelet-derived microparticles generated in vitro resemble circulating vesicles of patients with rheumatoid arthritis and activate monocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Villar-Vesga,Camilo Grajales,Catalina Burbano,Adriana Vanegas-García,Carlos H Muñoz-Vahos,Gloria Vásquez,Mauricio Rojas,Diana Castaño

Frontiers in cellular and infection microbiology 8:226 PubMed30023352

2018

Induce Signaling via Raf/MEK/ERK for Neutrophil Extracellular Trap (NET) Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Zayda Fonseca,César Díaz-Godínez,Nancy Mora,Omar R Alemán,Eileen Uribe-Querol,Julio C Carrero,Carlos Rosales

Frontiers in immunology 9:322 PubMed29545790

2018

Potential Involvement of Platelet-Derived Microparticles and Microparticles Forming Immune Complexes during Monocyte Activation in Patients with Systemic Lupus Erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Catalina Burbano,Juan Villar-Vesga,Janine Orejuela,Carlos Muñoz,Adriana Vanegas,Gloria Vásquez,Mauricio Rojas,Diana Castaño

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Citrulline antibody

Immunocytochemistry - Anti-Citrulline antibody (AB100932)

ELISA - Anti-Citrulline antibody (AB100932)

ELISA - Anti-Citrulline antibody (AB100932)

Key facts

Host species

Clonality

Isotype

Carrier free

Applications

Specificity

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Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

Product promise