Anti-CK1 epsilon antibody [1/Casein Kinase Iε]

4 product images

• 

  Western blot - Anti-CK1 epsilon antibody [1/Casein Kinase Iε] (ab302638)

  WB also tested the KO lysated side by side with published Rabmab (Anti-CK1 epsilon antibody [EPR24332-25] ab270997), please find the in internal WB.
  
  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  
  Exposure time: 3 minutes

  All lanes: Western blot - Anti-CK1 epsilon antibody [1/Casein Kinase Iε] (ab302638) at 1/1000 dilution

  Lane 1: 293T (human embryonic kidney epithelial cell), whole cell lysate

  Lane 2: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate

  Lane 3: U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate

  Lane 4: C2C12 (mouse myoblasts), whole cell lysate

  Lane 5: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate

  Lane 6: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

  Lane 7: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

  Secondary

  All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

  Exposure time: 3min

• 

  Immunocytochemistry/ Immunofluorescence - Anti-CK1 epsilon antibody [1/Casein Kinase Iε] (ab302638)

  Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma small irregularly shaped cells) cells labelling CK1 epsilon with ab302638 at 1/100 9.9ug/ml dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing mainly cytoplasmic staining in PC-12 cell line is observed. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 10ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
  
  Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-CK1 epsilon antibody [1/Casein Kinase Iε] (ab302638)

  Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling CK1 epsilon with ab302638 at 1/100 9.9ug/ml dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing mainly cytoplasmic staining in NIH/3T3 cell line is observed. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 10ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
  
  Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-CK1 epsilon antibody [1/Casein Kinase Iε] (ab302638)

  Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling CK1 epsilon with ab302638 at 1/100 9.9ug/ml dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing mainly cytoplasmic staining in HeLa cell line is observed. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 10ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
  
  Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.